A5091634068- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Rabies epidemiology and control
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Antibiotic Resistance in Bacteria
- Hemoglobin structure and function
- Viral Infections and Vectors
- Peptidase Inhibition and Analysis
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Viral Infections and Outbreaks Research
- Antibiotics Pharmacokinetics and Efficacy
- vaccines and immunoinformatics approaches
- Pneumonia and Respiratory Infections
- Virus-based gene therapy research
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Agriculture and Rural Development Research
- French Urban and Social Studies
- Neurological diseases and metabolism
- Prion Diseases and Protein Misfolding
- Trace Elements in Health
- Cellular transport and secretion
Centre National de la Recherche Scientifique
2010-2024
Université Grenoble Alpes
2010-2024
Institut de Biologie Structurale
2005-2024
CEA Grenoble
2000-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2000-2024
Université Joseph Fourier
2005-2020
Unit of Virus Host Cell Interactions
2007-2014
European Molecular Biology Laboratory
2011
Institut Laue-Langevin
2007
Brazilian Synchrotron Light Laboratory
2007
Summary In enterobacteria, the ampD gene encodes a cytosolic protein which acts as negative regulator of β‐lactamase expression. It is shown here that AmpD novel N ‐acetylmuramyl‐L‐alanine amidase (E.C.3.5.1.28) participating in intracellular recycling peptido‐glycan fragments. Surprisingly, exhibits an exclusive specificity for substrates containing anhydro muramic acid. This bond mainly found peptidoglycan degradation products formed by periplasmic lytic transglycosylases and thus might...
The genome of measles virus is encapsidated by multiple copies the nucleoprotein (N), forming helical nucleocapsids molecular mass approaching 150 Megadalton. intrinsically disordered C-terminal domain N (N TAIL ) essential for transcription and replication via interaction with phosphoprotein P viral polymerase complex. recognition element (MoRE) that binds situated 90 amino acids from folded RNA-binding CORE N, raising questions about functional role this chain. Here we report first in situ...
Replication of non-segmented negative-strand RNA viruses requires the continuous supply nucleoprotein (N) in form a complex with phosphoprotein (P). Here, we present structural characterization soluble, heterodimeric between variant vesicular stomatitis virus N lacking its 21 N-terminal residues (NΔ21) and peptide 60 amino acids (P60) encompassing molecular recognition element (MoRE) P that binds RNA-free (N0). The crystallized decameric circular form, which was solved at 3.0 Å resolution,...
Abstract Deciphering the mechanisms directing transcription factors (TFs) to specific genome regions is essential understand and predict transcriptional regulation. TFs recognize short DNA motifs primarily through their DNA-binding domain. Some also possess an oligomerization domain suspected potentiate binding but for which genome-wide influence remains poorly understood. Here we focus on LEAFY factor, a master regulator of flower development in angiosperms. We have determined crystal...
The pH 4 folding intermediate of apomyoglobin exists in two forms (Ia, Ib) at equilibrium. Their ratio depends on pH, urea concentration and the presence or absence a stabilizing anion (citrate, sulfate), it does not depend protein concentration. Ia Ib species are separated by kinetic barrier their interconversion can be monitored tryptophan fluorescence stopped-flow experiments. At 4.2, is converted to low concentrations unfolding gives transition Ia. During refolding native (N) 6, starting...
Hendra virus (HeV) is a recently emerged severe human pathogen that belongs to the Henipavirus genus within Paramyxoviridae family. The HeV genome encapsidated by nucleoprotein (N) helical nucleocapsid. Recruitment of viral polymerase onto nucleocapsid template relies on interaction between C-terminal domain, N(TAIL), N and X XD, co-factor phosphoprotein (P). Here, we provide an atomic resolution description intrinsically disordered N(TAIL) domain in its isolated state intact nucleocapsids...
The high-molecular-mass penicillin-binding proteins (HMM-PBPs), present in the cytoplasmic membranes of all eubacteria, are involved important physiological events such as cell elongation, septation or shape determination. Up to now it has, however, been very difficult impossible study catalytic properties HMM-PBPs vitro. With simple substrates, we could demonstrate that several these catalyse hydrolysis some thioesters transfer their acyl moiety on amino group a suitable acceptor...
The crystal structure of the dimerization domain rabies virus phosphoprotein was determined. monomer consists two alpha-helices that make a helical hairpin held together mainly by hydrophobic interactions. has hydrophilic and face, in dimer monomers pack through their surfaces. This is very different from vesicular stomatitis also tetramerization Sendai phosphoprotein, suggesting oligomerization conserved but not structure.
Background The phylum Crenarchaeota lacks the FtsZ cell division hallmark of bacteria and employs instead Cdv proteins. While CdvB CdvC are homologues eukaryotic ESCRT-III Vps4 proteins, implicated in membrane fission processes during multivesicular body biogenesis, cytokinesis budding some enveloped viruses, little is known about structure function CdvA. Here, we report biochemical biophysical characterization three proteins from hyperthermophilic archaeon Metallospherae sedula....
The catalytic pathway of class A β-lactamases involves an acyl-enzyme intermediate where the substrate is ester-linked to Ser-70 residue. Glu-166 and Lys-73 have been proposed as candidates for role general base in activation serine OH group. replacement by asparagine TEM-1 a histidine Streptomyces albus G yielded enzymes forming stable acyl-enzymes with β-lactam antibiotics. Although acylation modified proteins benzylpenicillin remained relatively fast, it was significantly impaired when...
Abstract The phosphoprotein (P) of vesicular stomatitis virus (VSV) interacts with nascent nucleoprotein (N), forming the N 0 –P complex that is indispensable for correct encapsidation newly synthesized viral RNA genome. In this complex, N‐terminal region (P NTR ) P prevents from binding to cellular and keeps it available encapsidating genomes. Here, using nuclear magnetic resonance (NMR) spectroscopy small‐angle X‐ray scattering (SAXS), we show an isolated peptide corresponding 60 first...
Massive mortalities of Carassius auratus (L.) occurred in a farm France during summer 2014. Fish presented anorexia, loss scales and large amounts mucus on the gills. Necrosis distal tip filament lamellae, combined with fusion was observed, as well necrosis hematopoietic organs digestive tract. The histological examination led to hypothesize implication virus mortality. presence cyprinid herpesvirus 2 (CyHV-2) dead fish demonstrated by amplification sequencing portions DNA polymerase...