A5022917136- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Berry genetics and cultivation research
- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
- Plant Virus Research Studies
- Plant biochemistry and biosynthesis
- Light effects on plants
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Plant Stress Responses and Tolerance
- Lipid metabolism and biosynthesis
- Turfgrass Adaptation and Management
- Ubiquitin and proteasome pathways
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Polysaccharides and Plant Cell Walls
- Biochemical Acid Research Studies
- RNA Research and Splicing
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"
2017-2025
Consejo Superior de Investigaciones Científicas
2014-2025
Universidad de Málaga
2014-2025
Andalusian Institute of Agricultural and Fisheries Research and Training
2019-2021
Max Planck Institute for Developmental Biology
2012-2017
Research International (United States)
2012
Wageningen University & Research
2012
Max Planck Society
2011
Chilly Repression Stalls Flowering In a cool spring, flowering might be delayed compared to warm even though the change in day length marches on regardless of temperature. Lee et al. (p. 628 , published online 12 September; see Perspective by Nilsson ) now show that this delay is regulated process, not simply consequence sluggish metabolism. model plant Arabidopsis transcription gene encoding regulator SHORT VEGETATIVE PHASE (SVP) unaffected temperature, but stability SVP protein decreased...
The fruits of diploid and octoploid strawberry (Fragaria spp) show substantial natural variation in color due to distinct anthocyanin accumulation distribution patterns. Anthocyanin biosynthesis is controlled by a clade R2R3 MYB transcription factors, among which MYB10 the main activator fruit. Here, we that mutations cause most observed woodland (F. vesca) cultivated ×ananassa). Using mapping-by-sequencing approach, identified gypsy-transposon truncates protein knocks out white-fruited F....
In contrast to climacteric fruits such as tomato, the knowledge on key regulatory genes controlling ripening of strawberry, a nonclimacteric fruit, is still limited. NAC transcription factors (TFs) mediate different developmental processes in plants. Here, we identified and characterized Ripening Inducing Factor (FaRIF), TF that highly expressed induced strawberry receptacles during ripening. Functional analyses based stable transgenic lines aimed at silencing FaRIF by RNA interference,...
Abstract The transition from vegetative to reproductive development is one of the most important phase changes in plant life cycle. This step controlled by various environmental signals that are integrated at molecular level so-called floral integrators. One such integrator Arabidopsis (Arabidopsis thaliana) MADS domain transcription factor SUPPRESSOR OF OVEREXPRESSION CONSTANS1 (SOC1). Despite extensive genetic studies, little known about transcriptional control SOC1, and we just starting...
Abstract Despite great advances in sequencing technologies, generating functional information for nonmodel organisms remains a challenge. One solution lies an improved ability to predict genetic circuits based on primary DNA sequence combination with detailed knowledge of regulatory proteins that have been characterized model species. Here, we focus the LEAFY (LFY) transcription factor, conserved master regulator floral development. Starting biochemical and structural information, built...
Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3-oxidosqualene, precursor cyclic triterpenoids. Here we report that Arabidopsis drought hypersensitive/squalene 1-5 (dry2/sqe1-5) mutant, identified by its extreme hypersensitivity to stress, has altered stomatal responses and root defects because a point mutation in SQUALENE EPOXIDASE 1 (SQE1) gene. GC-MS analysis indicated dry2/sqe1-5 mutant sterol composition roots but wild-type shoots, indicating an essential role for...
Abstract RNA-seq has been used to perform global expression analysis of the achene and receptacle at four stages fruit ripening, roots leaves strawberry ( Fragaria × ananassa ). About 967 million reads 191 Gb sequence were produced, using Illumina sequencing. Mapping in related genome wild diploid vesca revealed differences between development program, reinforced role played by ethylene ripening receptacle. For transcriptome assembly, a de novo strategy was followed, generating separate...
Plants integrate seasonal cues such as temperature and day length to optimally adjust their flowering time the environment. Compared control of before after winter by vernalization pathways, mechanisms that delay or promote during a transient cool warm period, especially spring, are less well understood. Due global warming, understanding this ambient pathway has gained increasing importance. In Arabidopsis thaliana, FLOWERING LOCUS M (FLM) is critical regulator pathway. FLM alternatively...
Abstract Deciphering the mechanisms directing transcription factors (TFs) to specific genome regions is essential understand and predict transcriptional regulation. TFs recognize short DNA motifs primarily through their DNA-binding domain. Some also possess an oligomerization domain suspected potentiate binding but for which genome-wide influence remains poorly understood. Here we focus on LEAFY factor, a master regulator of flower development in angiosperms. We have determined crystal...
The B-class of MADS-box transcription factors has been studied in many plant species, but remains functionally uncharacterized Rosaceae. APETALA3 (AP3), a member this class, controls petal and stamen identities Arabidopsis. In study, we identified two members the AP3 lineage cultivated strawberry, Fragaria × ananassa, namely FaAP3 FaTM6. FaTM6, not FaAP3, showed an expression pattern equivalent to that We used CRISPR/Cas9 genome editing system for first time octoploid species characterize...
Abstract Plants can produce organs throughout their entire life from pluripotent stem cells located at growing tip, the shoot apical meristem (SAM). At time of flowering, SAM Arabidopsis thaliana switches fate and starts producing flowers instead leaves. Correct timing flowering in part determines reproductive success, is therefore under environmental endogenous control. How epigenetic regulation contributes to floral transition has eluded analysis so far, mostly because poor accessibility...
The NAC transcription factor ripening inducing (RIF) was previously reported to be necessary for the of octoploid strawberry (Fragaria × ananassa) fruit, but mechanistic basis RIF-mediated transcriptional regulation and how RIF activity is modulated remains elusive. Here, we show that FvRIF in diploid strawberry, Fragaria vesca, a key regulator control fruit knockout mutations result complete block ripening. DNA affinity purification sequencing coupled with transcriptome deep suggests 2,080...
The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) enzyme catalyzes the major rate-limiting step of mevalonic acid (MVA) pathway from which sterols and other isoprenoids are synthesized. In contrast with our extensive knowledge regulation HMGR in yeast animals, little is known about this process plants. To identify regulatory components MVA plants, we performed a genetic screen for second-site suppressor mutations Arabidopsis thaliana highly drought-sensitive drought hypersensitive2 (dry2)...
Brassinosteroids (BRs) form a group of steroidal hormones essential for plant growth, development, and stress responses. BRs are perceived extracellularly by plasma membrane receptor-like kinases that activate an interconnected signal transduction cascade, leading to the transcriptional regulation BR-responsive genes. TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) genes specific land plants, their encoded proteins defined presence protein–protein interaction motives, is, intrinsic disordered...
Various environmental signals integrate into a network of floral regulatory genes leading to the final decision on when flower. Although wealth qualitative knowledge is available how flowering time regulate each other, only few studies incorporated this predictive models. Such models are invaluable as they enable investigate various types inputs combined give quantitative readout. To effect gene expression disturbances time, we developed dynamic model for regulation in Arabidopsis thaliana....
ABSTRACT Ripening Inducing Factor (RIF) is a key NAC transcription factor regulating strawberry fruit ripening. Previous studies using RIF- RNAi and overexpression lines in Fragaria × ananassa CRISPR knock-out F. vesca have established the role of RIF controlling ABA biosynthesis signaling, cell wall remodeling, secondary metabolism. In this study, we deciphered FaRIF’s transcriptional regulatory network by combining ChIP-seq-based identification its direct targets with an analysis FaRIF...
Climate change is causing increasing temperatures and drought, creating new environmental conditions, which species must cope with. Plant can respond to these shifting environments by escaping more favorable environments, undergoing adaptive evolution, or exhibiting phenotypic plasticity. In this study, we investigate genotype responses variation in conditions (genotype-by-environment interactions; G × E) over multiple years gain insights into the plasticity potential of plants changes face...
TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) proteins are characterized by the presence of six tetratricopeptide repeats in conserved positions and a carboxyl-terminal region known as thioredoxin-like domain with homology to thioredoxins. In Arabidopsis (Arabidopsis thaliana), TTL gene family is composed four members, founder member, TTL1, required for osmotic stress tolerance. Analysis sequenced genomes indicates that genes specific land plants. this study, we report expression profiles using...
Plant life-long organogenesis involves sequential, time and tissue specific expression of developmental genes. This requires activities Polycomb Group (PcG) trithorax complexes (trxG), respectively responsible for repressive Histone 3 trimethylation at lysine 27 (H3K27me3) activation-related 4 (H3K4me3). However, the genome-wide dynamics in histone modifications that occur during processes have remained elusive. Here, we report distributions H3K27me3 H3K4me3 along with changes, a series...
Over the last few years, a series of tools for genome editing have been developed, allowing introduction precise changes into plant genomes. These included Zinc-finger nucleases (ZFNs), transcription activator-like effector (TALENs), and CRISPR/Cas9, which is so far most successful commonly used approach targeted stable DNA, due to its ease use low cost. CRISPR/Cas9 now being widely as new breeding technique improve commercially relevant crop species. Fruit ripening complex genetically...