A5028273586- Dermatology and Skin Diseases
- Contact Dermatitis and Allergies
- Urticaria and Related Conditions
- Drug-Induced Adverse Reactions
- Autoimmune Bullous Skin Diseases
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Animal Genetics and Reproduction
- Immune Cell Function and Interaction
- Mast cells and histamine
- Psoriasis: Treatment and Pathogenesis
- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- Occupational exposure and asthma
- Skin and Cellular Biology Research
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Skin Protection and Aging
- Virus-based gene therapy research
- Eosinophilic Disorders and Syndromes
- Immune Response and Inflammation
Centre International de Recherche en Infectiologie
2016-2025
Inserm
2016-2025
Université Claude Bernard Lyon 1
2016-2025
Hospices Civils de Lyon
2016-2025
Hôpital Lyon Sud
2015-2025
Mútua Terrassa
2019-2025
Laboratoire d'Informatique en Images et Systèmes d'Information
2025
International Laboratory on Learning Systems
2025
Institut National des Sciences Appliquées de Lyon
2025
McGill University
2025
We demonstrate that individual cells infected with and expressing a recombinant retrovirus carrying the Escherichia coli beta-galactosidase gene (lacZ) can be viably stained, analyzed, sorted, cloned by fluorescence-activated cell sorting based on levels of lacZ expressed. To accomplish this we have devised method to enzymatically generate maintain fluorescence in live mammalian cells. Accumulation fluorescent products is linear time, direct correlation enzymatic activity. This technology...
The mitochondrial intron-encoded endonuclease I-SceI of Saccharomyces cerevisiae has an 18-bp recognition sequence and, therefore, a very low probability cutting DNA, even within large genomes.We demonstrate that double-strand breaks can be initiated by the at predetermined location in mouse genome and repaired with donor molecule homologous regions flanking breaks.This induced recombination is approximately 2 orders magnitude more frequent than spontaneous least 10 times random integration...
Contact sensitivity (CS) is a form of delayed-type hypersensitivity to haptens applied epicutaneously and thought be mediated, like classical responses, by CD4+ T helper-1 cells. The aim this study was identify the effector cells involved in CS. We studied CS strongly sensitizing hapten dinitrofluorobenzene (DNFB) mice rendered deficient homologous recombination either major histocompatibility complex (MHC) class I, MHC II, or both, which exhibited deficiencies in, respectively, CD8+, CD4+,...
Contact hypersensitivity (CHS) is a T cell–mediated skin inflammation induced by epicutaneous exposure to haptens in sensitized individuals. We have previously reported that CHS dinitrofluorobenzene mice mediated major histocompatibility complex (MHC) class I–restricted CD8+ cells. In this study, we show cells mediate the through their cytotoxic activity. The contribution of specific lymphocytes (CTLs) reaction was examined both vivo and vitro, using deficient perforin and/or Fas/Fas ligand...
The Escherichia coli lacZ gene has been used as an indicator for the study of cell lineage in vivo. To adapt this marker expression studies, a sequence encoding modified beta-galactosidase and including simian virus 40 large tumor nuclear location signal (nls-beta-Gal) introduced into vectors. In differentiated cells, multipotential embryos, constructs led to enzymatically active protein. Its was examined by its activity or using antibodies electron microscopy. results show that nls-beta-Gal...
Abstract Contact hypersensitivity (CHS) is a T cell-mediated, Ag-specific skin inflammation induced by exposure to haptens in sensitized individuals. Th1/T cytotoxic 1 cells are effector of CHS, whereas Th2/T regulatory CD4+ have down-regulating properties. We previously shown that CHS 2,4-dinitrofluorobenzene mediated specific CD8+ cells, whose cytolytic activity mandatory for induction inflammation. In this study, using immunohistochemistry and RT-PCR analysis, we show rapidly recruited...
Head muscle progenitors in pharyngeal mesoderm are present close proximity to cells of the second heart field and show overlapping patterns gene expression. However, it is not clear whether a single progenitor cell gives rise both head muscles. We now that this case, using retrospective clonal analysis which an nlaacZ sequence, converted functional nlacZ after rare intragenic recombination event, targeted αc-actin gene, expressed all developing skeletal cardiac muscle. distinguish two...
The genome of measles virus is encapsidated by multiple copies the nucleoprotein (N), forming helical nucleocapsids molecular mass approaching 150 Megadalton. intrinsically disordered C-terminal domain N (N TAIL ) essential for transcription and replication via interaction with phosphoprotein P viral polymerase complex. recognition element (MoRE) that binds situated 90 amino acids from folded RNA-binding CORE N, raising questions about functional role this chain. Here we report first in situ...
From embryonal carcinoma cells labeled with fucose, two main classes of glycopeptide products Pronase digestion can be distinguished by Sephadex G-50 column chromatography: one eluted near the excluded volume and a smaller one. The large fucosyl-glycopeptides are scarcely present in differentiated derived from (i.e., fibroblastlike cells, myoblasts, parietal yolk-sac carcinoma). During vitro differentiation these glycopeptides disappear almost completely. small were analyzed paper...
Porcine skin is increasingly being employed as a model of human in various research fields, including pharmacology, toxicology and immunology, with particular interest percutaneous permeation organ transplantation. shows several anatomical physiological similarities, but also some differences, skin, few depth comparative studies are so far available. To study the immunohistochemical properties normal porcine comparison skin. We performed histological on frozen formalin-fixed,...
Drug allergic reactions presenting as maculo-papular exanthema (MPE) are mediated by drug-specific T cells. In this study, the frequency of circulating specific cells was analyzed interferon-gamma (IFN-gamma) enzyme-linked immunospot assay in 22 patients with an MPE to amoxicillin (amox). Amox-specific were detected 20/22 frequencies ranging from 1 : 8000 30 000 leucocytes. No reactivity observed 46 control patients, including 15 immunoglobulin E-mediated allergy amoxicillin, 11 a history...
Abstract Background WHIM syndrome (WS), a rare congenital neutropenia due to mutations of the CXCR4 chemokine receptor, is associated with Human Papillomavirus (HPV)-induced Warts, Hypogammaglobulinemia, bacterial Infections and Myelokathexis. The long term follow up eight patients highlights clinical heterogeneity this disease as well main therapeutic approaches remaining challenges in light recent development new inhibitors. Objective This study aims describe natural history WS based on...