A5054094510- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Drug-Induced Adverse Reactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Parasitic Infections and Diagnostics
- Antimicrobial Resistance in Staphylococcus
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Cystic Fibrosis Research Advances
- Animal Disease Management and Epidemiology
- Asthma and respiratory diseases
- Pediatric health and respiratory diseases
- Virus-based gene therapy research
- Contact Dermatitis and Allergies
- Toxin Mechanisms and Immunotoxins
- Food Allergy and Anaphylaxis Research
- HIV Research and Treatment
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Reproductive System and Pregnancy
- Pharmaceutical studies and practices
- Immune Response and Inflammation
- Urticaria and Related Conditions
- Mosquito-borne diseases and control
Hôpital Edouard Herriot
1988-2018
Université Claude Bernard Lyon 1
1991-2016
Inserm
1990-2009
Hôpital Lyon Sud
2002-2009
Hospices Civils de Lyon
2001-2009
Centre National de la Recherche Scientifique
1988-2007
Institut Pasteur
2006-2007
Hôpital de la Croix-Rousse
1998-2007
Laboratoire de Biométrie et Biologie Evolutive
2007
Laboratoire National de Référence
2004
We investigated the involvement of recently described staphylococcal enterotoxins G and I in toxic shock syndrome. reexamined Staphylococcus aureus strains isolated from patients with menstrual nonmenstrual syndrome (nine cases) or scarlet fever (three cases). These were selected because they produced none toxins known to be involved these syndromes (toxic toxin 1 A, B, C, D), enterotoxin E H, exfoliative A despite fact that superantigenic detected a CD69-specific flow cytometry assay...
Panton Valentine leukocidin (PVL) may be responsible for pulmonary necrosis in necrotizing Staphylococcus aureus pneumonia, a highly lethal infection. Commercial intravenous immunoglobulin (IVIg) preparations containing antibodies against PVL might have therapeutic value this setting, as an adjunct to antimicrobial chemotherapy. To test possibility, we determined anti-PVL antibody titers commercial IVIg and the capacity of prevent cytopathic effects vitro. Specific enzyme-linked...
ABSTRACT To test the hypothesis that Staphylococcus aureus enterotoxin gene cluster ( egc ) can generate new genes by recombination, we analyzed locus in a broad panel of 666 clinical isolates S. aureus. was present 63% isolates, confirming its high prevalence. The archetypal organization locus, consisting five plus two pseudogenes, found 409 421 -positive strains. incomplete few strains and occasionally harbored an insertion sequence transposase genes. These may represent evolutionary...
Drug allergic reactions presenting as maculo-papular exanthema (MPE) are mediated by drug-specific T cells. In this study, the frequency of circulating specific cells was analyzed interferon-gamma (IFN-gamma) enzyme-linked immunospot assay in 22 patients with an MPE to amoxicillin (amox). Amox-specific were detected 20/22 frequencies ranging from 1 : 8000 30 000 leucocytes. No reactivity observed 46 control patients, including 15 immunoglobulin E-mediated allergy amoxicillin, 11 a history...
ABSTRACT With the use of Weber’s modified trichrome and Uvitex 2B techniques, spores microsporidia were detected in stools four travelers presenting clinically with chronic diarrhea. The general health these patients was not impaired, human immunodeficiency virus screening negative. Immune evaluation, including study lymphocytic subpopulations, assay serum immunoglobulins, an intradermal multitest, showed normal results. Molecular identification microsporidian species based on PCR...
<i>Background:</i> Quinolone hypersensitivity reactions are being more frequently reported. Skin tests in investigations of patients known to not be fully reliable. The provocation test thus remains the gold standard definitive diagnosis allergy, despite risks involved. aim this study was evaluate basophil activation (BATs) immediate-type quinolones. <i>Methods:</i> Thirty-four who presented an hypersensivity reaction less than hour after quinolone administration were...
IgG replacement therapy (IgRT) in primary immunodeficiencies (PID) is a lifelong treatment which may be administered intravenously (IVIg) or subcutaneously (SCIg), at hospital home. The objective of the VISAGE study was to investigate if route and/or place for IgRT impact patients' satisfaction regarding and quality life (QoL) real-life conditions.The enrolled PID patients least 15 years old receiving 3 months. Satisfaction QoL were evaluated enrollment over 12-month follow-up period by Life...
Journal Article Secretory IgA Antibodies to Cryptosporidium parvum in AIDS patients with Chronic Cryptosporidiosis Get access Grégoire Cozon, Cozon Reprints or correspondence: Dr. INSERM U80, Hôpital E. Herriot pavilion P, 69437 Lyon Cedex 3, France. Search for other works by this author on: Oxford Academic PubMed Google Scholar François Biron, Biron Michèle Jeannin, Jeannin Dominique Cannella, Cannella Jean-Pierre Revillard The of Infectious Diseases, Volume 169, Issue March 1994, Pages...
The objective of the present study was to investigate maturation immunoglobulin G (IgG) avidity after Toxoplasma gondii seroconversion during pregnancy and factors that affect IgG over time. used 309 serum samples from 117 women a multiple linear mixed regression analysis show patterns variation throughout gestation. ratios their evolution with time were quite diverse among statistically heterogeneous (P = 0.011); however, trend toward significant increase < 0.0001). On average, 1.0167-fold...
The parasite Toxoplasma gondii can infect most mammals and birds, sometimes causing severe pathology. Primary infection during pregnancy result in abortion or fetal defects. Host immunity, particularly cellular immunity towards antigenic peptides, control infection, but an efficient vaccine is not yet available. We have evaluated T-cell responses to a crude soluble toxoplasma antigen (ST-Ag) five recombinant peptide antigens of cells whole-blood cultures from 22 pregnant women with...
Summary Humoral immune response is essential for protection against invasive pneumococcal disease and this property the basis of polysaccharide-based anti-pneumococcal vaccines. Pneumococcal surface protein A (PspA), a cell-wall-associated protein, promising component next generation This PspA antigen has been shown to stimulate an antibody-based immunity. In present study, we evaluated capacity CD4+ T cells which are needed correct development B cell based in humans. Cellular immunity was...
SUMMARY In order to investigate the contribution of lymphocytes interstitial lung disease in animals with visna-maedi infection, we studied parallel bronchoalveolar cells and tissue from slaughterhouse (n = 29) colostrum-deprived lambs transtracheally inoculated field isolates virus 9) or saline 6). Lymphocyte subpopulations were identified lavagc by immunofluorescence flow cylomctry analysis using indirect immunohistochemistry. infected a lymphocytic alveolitis containing CD4 CD8 was...
The presence of staphylococcal superantigenic toxins in the supernatants liquid cultures was detected by an easy and rapid method assessing activation T lymphocytes cytofluorimetric measurement CD69 expression. Staphylococcus aureus cells were grown Eagle's minimum essential medium supplemented with 5% heat-inactivated fetal calf serum. Supernatant fluids from all S. strains producing superantigen-related toxins, including enterotoxins A to E, toxic shock syndrome toxin, exfoliative B,...
Abstract The diagnosis of toxoplasmosis relies primarily on the demonstration specific antibodies to Toxoplasma gondii . We describe a flow cytometry method for determination whole fixed tachyzoites by indirect immunofluorescence. Fixed in suspension have characteristic light scattering properties. amount IgM, IgG, and IgA from patients' sera bound can be estimated mean fluorescence intensities observed using class‐specific conjugates different Ig heavy chains. Appropriate serum dilutions...
The complex interactions between nutrition, infection and immunity are still poorly understood the precise role of each factor is difficult to ascertain. Infection itself induces profound metabolic alterations resulting in negative nitrogen balance. Protein energy malnutrition, whatever its origin, results an immune deficiency characterized by impaired delayed cutaneous hypersensitivity, low production lymphokines, T cell counts, decreased responses specific or non mitogenic stimuli. Serum...
SUMMARY Protection against Toxoplasma gondii in infected patients is mainly attributed to cellular immunity. We here attempt improve the characterization of proteins that induce immunity naturally patients. Cellular was evaluated by flow cytometry after 7 days blood culture from 31 chronically T. and 8 noninfected pregnant women, presence soluble antigen (ST-Ag) or fractionated ST-Ag, separated sodium dodecyl sulphate polyacrylamide gel electrophoresis. Blood cultures with ST-Ag induced 39·5...
Subcutaneous immunoglobulin replacement therapy (IgRT) may be administered once a week with pump or every other day syringe (rapid push). The objective of the study was to compare impact and rapid push infusions on patient's life quality index (LQI). This randomized, crossover, multicenter, non-inferiority trial conducted in adults primary immunodeficiency (PID) accustomed weekly at home by pump. Patients used for 3 months each according randomized sequence. Main criterion PID-LQI factor I...
ABSTRACT To assess cell-mediated immunity to Toxoplasma gondii , we evaluated the expression of activation antigens CD69, CD71, and CD25 on T lymphocytes by flow cytometry after specific in vitro stimulation whole blood from 127 T. -positive 63 -negative patients. many seropositive individuals did not express CD69 at 24 h antigen stimulation, but CD71 were easily detectable cells 7 days activation. was mainly expressed stimulated CD4 + cells, its detection total both a sensitive (98%) (97%)...