Intrauterine infection is a recognized cause of adverse pregnancy outcome, but the source often undetermined. We report case stillbirth caused by Fusobacterium nucleatum that originated in mother's mouth.A woman with pregnancy-associated gingivitis experienced an upper respiratory tract at term, followed few days later. F. was isolated from placenta and fetus. Examination different microbial floras mother identified same clone her subgingival plaque not supragingival plaque, vagina, or...

ABSTRACT Microbial infection of the intrauterine environment is a major cause preterm birth. The current paradigm indicates that infections predominantly originate from vaginal tract, with organisms ascending into sterile uterus. With improvements in technology, an increasing number bacterial species have been identified do not belong to microflora. We demonstrated previously can oral cavity following hematogenous transmission. In this study, we begin systemically examine what proportion...

O-linked N-acetylglucosaminylation (O-GlcNAcylation) is a reversible post-translational modification consisting in the addition of sugar moiety to serine/threonine residues cytosolic or nuclear proteins. Catalyzed by O-GlcNAc-transferase (OGT) and removed O-GlcNAcase, this dynamic dependent on environmental glucose concentration. O-GlcNAcylation regulates activities wide panel proteins involved almost all aspects cell biology. As nutrient sensor, can relay effects excessive nutritional...

Fusobacterium nucleatum is a Gram-negative oral anaerobe, capable of systemic dissemination causing infections and abscesses, often in mixed-species, at different body sites. We have shown previously that F. adheres to invades host epithelial endothelial cells via novel FadA adhesin. In this study, vascular (VE)-cadherin, member the cadherin family cell-cell junction molecule, was identified as receptor for FadA, required binding cells. colocalized with VE-cadherin on cells, relocation away...

O-GlcNAcylation (addition of N-acetyl-glucosamine on serine or threonine residues) is a post-translational modification that regulates stability, activity localization cytosolic and nuclear proteins. O-linked N-acetylgluocosmaine transferase (OGT) uses UDP-GlcNAc, produced in the hexosamine biosynthetic pathway to O-GlcNacylate Removal O-GlcNAc from proteins catalyzed by β-N-Acetylglucosaminidase (OGA). Recent evidences suggest may affect growth cancer cells. However, consequences...

ABSTRACT Salmonella enterica , like many gram-negative pathogens, uses type three secretion systems (TTSS) to infect its hosts. The TTSS of namely, TTSS-1, TTSS-2, and flagella, play a major role in the virulence this bacterium, allowing it cross intestinal barrier disseminate systemically. Previous data from our laboratory have demonstrated involvement chromosomal region harboring yfgL engA yfgJ open reading frames S. serovar Enteritidis virulence. Using microarray analysis real-time...

O-GlcNAcylation on serine/threonine is a post-translational modification that controls the activity of nucleocytoplasmic proteins according to glucose availability. We previously showed FoxO1 in liver cells increases its transcriptional activity. In present study, we evaluated potential involvement context pancreatic β-cell glucotoxicity. was O-GlcNAcylated INS-1 832/13 β and isolated rat islets. resulted 2-fold increase toward reporter gene 3-fold expression insulin-like growth...

The viral Tax oncoprotein plays a key role in both Human T-cell lymphotropic virus type 1 (HTLV-1)-replication and HTLV-1-associated pathologies, notably adult leukemia. governs the transcription from 5'LTR, enhancing thereby its own expression, via recruitment of dimers phosphorylated CREB to cAMP-response elements located within U3 region (vCRE). In addition phosphorylation, is also target O-GlcNAcylation, another reversible post-translational modification involved wide range diseases,...

In order to understand the causes of low virulence Listeria monocytogenes field strains, five low-virulence strains were analysed. These showed changes in relation invasion, phosphatidyl-inositol phospholipase C (PI-PLC) activity, plaque formation and vivo virulence. Molecular analyses revealed same mutations plcA, inlA inlB genes all strains. The Thr262Ala substitution PI-PLC protein was responsible for absence activity. This residue, conserved certain L. species, is located at outer rim...

In Escherichia coli, the assembly of outer-membrane proteins (OMP) requires BAM complex and periplasmic chaperones, such as SurA or DegP. Previous work has suggested a potential link between OMP expression genes encoding type-III secretion systems. order to test this hypothesis, we studied role different lipoproteins (i.e. BamB, BamC, BamD BamE), chaperones DegP, in these two phenotypes Salmonella . Analysis corresponding deletion mutants showed that, previously described with Δ bamB mutant,...

Thioredoxin interacting protein (TxNIP), which strongly responds to glucose, has emerged as a central mediator of glucotoxicity in pancreatic β cells. TxNIP is scaffold with target proteins inhibit or stimulate their activity. Recent studies reported that high glucose stimulates the interaction inflammasome NLRP3 (NLR family, pyrin domain containing 3) increase interleukin-1 (IL1β) secretion by To better understand regulation cells, we investigated implication O-linked β-N-acetylglucosamine...

To describe the characteristics of patients suffering from secondary immunodeficiencies (SID) associated with hematological malignancies (HM), who started immunoglobulin replacement therapy (IgRT), physicians' expectations regarding IgRT, and IgRT modalities.Non-interventional, prospective French cross-sectional study.The analysis included 231 (66 ± 12 years old) multiple myeloma (MM) (N = 64), chronic lymphoid leukemia (CLL) 84), aggressive non-Hodgkin B-cell lymphoma (aNHL) 32), indolent...

Objective: Immunoglobulin replacement therapy (IgRT) is increasingly used in secondary immunodeficiency (SID) related to hematological malignancies (HM) prevent infections. Study's objective was document prospectively the efficacy and safety of IgRT patients with HM-associated SID.Methods: Non-interventional, prospective French longitudinal study.Results: One-hundred sixty starting for SID (myeloma: 54 cases, chronic lymphoid leukemia: 54, aggressive non-Hodgkin B-cell lymphoma: 19, indolent...

The folding and insertion of β-barrel proteins in the outer membrane Gram-negative bacteria is mediated by BAM complex, which composed protein BamA four lipoproteins BamB to BamE. In Escherichia coli and/or Salmonella, lipoprotein involved (i) assembly membrane, (ii) permeability antibiotics, (iii) control expression T3SS are major virulence factors (iv) Salmonella. E. coli, this has been shown interact directly with BamA. study, we investigated structure-function relationship order assess...

The purpose of the APRIM study (for Adherence Polyarthrite Rhumatoïde Injection Methotrexate) was to investigate change in treatment adherence patients with rheumatic arthritis (RA) who switched from oral subcutaneous methotrexate (MTX).Prospective, observational RA treated MTX and switching (SC) route real-life conditions. Data on motivations for switch, disease activity (DAS28-CRP), quality life (AISM-2 SF), disability (HAQ-DI), were collected at inclusion (M0) 6 months later (M6)....

Subcutaneous immunoglobulin replacement therapy (IgRT) may be administered once a week with pump or every other day syringe (rapid push). The objective of the study was to compare impact and rapid push infusions on patient's life quality index (LQI). This randomized, crossover, multicenter, non-inferiority trial conducted in adults primary immunodeficiency (PID) accustomed weekly at home by pump. Patients used for 3 months each according randomized sequence. Main criterion PID-LQI factor I...

Patients with primary immunodeficiency (PID) often receive immunoglobulin replacement therapy (IgRT). Physicians and patients have the choice between various methods of administration. For subcutaneous infusions, may use an automated pump (P) or push plunger a syringe (rapid [RP]). P infusions are performed once week last around 1 hour. RP decreases duration administration, but requires more frequent infusions.Eight out 30 (coming from single center) who had participated in cross-over,...

Immunoglobulins are 2nd or 3rd-line treatments in dermatomyositis (DM) polymyositis (PM) refractory to high-dose corticosteroids and immunosuppressants. (2 g/kg/mo) usually administered intravenously (IVIg) once a month the patients stay at hospital for few days. Recently, subcutaneous injections (SCIg) were proposed 2 3 times per week, some dysimmune diseases. SCIg home preferably by patient nurse. We investigated needs attitudes of DM PM with experience IVIg SCIg.Seven (6 1 DM) from single...

Insulin analogues are largely used for the treatment of diabetic patients, but concerns have been raised about their mitogenic/anti-apoptotic potential. It is therefore important to evaluate these in different cell systems.The aim this work was establish pharmacological profiles insulin towards PI-3 kinase/Akt pathway INS-1 β-pancreatic cells.Bioluminescence Resonance Energy Transfer (BRET), western and caspase 3/7 assays, study effects ligands.Among five evaluated, only glargine stimulated...

The first-line treatment for nonspecific low back pain (LBP) relief is physical exercise; however, there no uniformity in recommendations regarding the type of exercise, and physicians predominantly prescribe pharmacological treatments. This creates a gap non-pharmacological management LBP. Preliminary data suggest that manual therapy acupressure could be relevant therapeutic options. primary objective this study was to describe evolution patients with LBP persisting at least 4 weeks who...

ChREBP ( Carbohydrate Responsive Element Binding Protein ) has emerged as a major molecular determinant of lipogenic gene expression in response to glucose liver cells. However, the mechanisms activation by remain complex. We identified that is modified post‐translational modifications including acetylation and O‐ GlcNAcylation glucose. O‐GlcNAcylation reversible modification depends on concentration its flux through hexosamine biosynthetic pathway (HBP). highly dynamic process, controlled...