A5002158278- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Cholesterol and Lipid Metabolism
- Diet and metabolism studies
- Digestive system and related health
- Adipokines, Inflammation, and Metabolic Diseases
- Diet, Metabolism, and Disease
- Diabetes Treatment and Management
- Gut microbiota and health
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Helicobacter pylori-related gastroenterology studies
- Cancer Cells and Metastasis
- Dietary Effects on Health
- Drug Transport and Resistance Mechanisms
- Diabetes and associated disorders
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Lipid metabolism and disorders
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- Pancreatic and Hepatic Oncology Research
Centre National de la Recherche Scientifique
2013-2024
Institut Cochin
2015-2024
Université Paris Cité
2013-2024
Inserm
2014-2024
Délégation Paris 5
2013-2020
Sorbonne Paris Cité
2013-2020
Délégation Paris 7
2015
Diabetes Australia
2015
Centre de Gestion Scientifique
2013
Montefiore Medical Center
2007-2009
Abstract Cancer cells integrate multiple biosynthetic demands to drive unrestricted proliferation. How these cellular processes crosstalk fuel cancer cell growth is still not fully understood. Here, we uncover the mechanisms by which transcription factor Carbohydrate responsive element binding protein (ChREBP) functions as an oncogene during hepatocellular carcinoma (HCC) development. Mechanistically, ChREBP triggers expression of PI3K regulatory subunit p85α, sustain activity pro-oncogenic...
Dietary proteins are mostly absorbed as di- and tripeptides by the intestinal proton-dependent transporter PepT1. We have examined effects of leptin on PepT1 function in rat jejunum monolayers human enterocyte-like 2 cell Caco-2. Leptin is produced stomach secreted gut lumen. show here that receptors expressed Caco-2 mucosal cells. Apical (but not basolateral) increased transport cephalexin (CFX) glycylsarcosine (Gly-Sar), an effect was associated with Gly-Sar uptake, membrane protein,...
Abstract Leptin, the product of ob gene, plays a key role in regulation food intake via cross‐talk between hypothalamic leptin receptors and neuropeptides that affect feeding behaviour. Recent studies have shown synergistic interaction cholecystokinin (CCK) leading to suppression intake, which involves CCK‐1 capsaicin‐sensitive vagal fibres. In this study, we investigated presence afferent efferent neurons vagus nerve. By using reverse transcription‐polymerase chain reaction, mRNAs encoding...
While the physiological benefits of fibroblast growth factor 21 (FGF21) hepatokine are documented in response to fasting, little information is available on Fgf21 regulation a glucose-overload context. We report that peroxisome-proliferator-activated receptor α (PPARα), nuclear fasting response, required with carbohydrate-sensitive transcription carbohydrate-responsive element-binding protein (ChREBP) balance FGF21 glucose response. Microarray analysis indicated only few hepatic genes...
This study shows that leptin induced a rapid phosphorylation of p42/44 mitogen-activated protein kinase, an enhancement both NF-κB DNA binding and transcriptional activities, concentration-dependent increase HT-29 cell proliferation. These effects are consistent with the presence receptors on membranes. The induction growth was associated population in S G2/M phase compared control cells found G0/G1 cycle. Moreover, cyclin D1 immunoreactivity enhanced leptin-treated this essentially phase....
Some of the actions leptin depend on cholecystokinin (CCK). However, it is unknown whether modulates release CCK. Here, we demonstrate in vitro that induces phosphorylation extracellular signal–related kinase (ERK)-1/2 proteins and increases CCK (EC50 = 0.23 nmol/l) CCK-secreting STC-1 cells. We showed rat duodenal juice contains circulates free bound to macromolecules, suggesting has a lumenal action intestine. In vivo rat, infusion increased plasma at levels comparable those induced by...
The effect of leptin on glucose transport was studied in rat jejunal mucosa Ussing chambers. Leptin added the luminal or serosal compartment before tissues were challenged with 1, 10, 50 mmol/l glucose. In response to 10 glucose, increase short-circuit current (ΔIsc) reached 26.8 ± 2.1 μA/cm2. Luminal addition dramatically decreased glucose-induced Isc (90.5% for nmol/l leptin). Inhibition maximal after 5 min and dose dependent (IC50 = 0.13 nM). Western blot analysis showed that rapid...
A common feature of metabolic diseases is their association with chronic low-grade inflammation. While enhanced gut permeability and systemic bacterial endotoxin translocation have been suggested as key players this metaflammation, the mechanistic bases underlying these features upon diabesity cascade remain partly understood.
Although the mechanisms by which glucose regulates insulin secretion from pancreatic β-cells are now well described, way modulates gene expression in such cells needs more understanding. Here, we demonstrate that MondoA, but not its paralog carbohydrate-responsive element–binding protein, is predominant glucose-responsive transcription factor human β-EndoC-βH1 and islets. In high-glucose conditions, MondoA shuttles to nucleus where it required for induction of genes arrestin...
Colorectal cancer (CRC) is associated with metabolic and redox perturbation. The mitochondrial transporter uncoupling protein 2 (UCP2) controls cell proliferation in vitro through the modulation of cellular metabolism, but underlying mechanism tumors vivo remains unexplored. Using murine intestinal models CRC patient samples, we find higher UCP2 levels compared to their non-tumoral counterparts. We reveal tumor-suppressive role as its deletion enhances colon small tumorigenesis...
Leptin has been suggested to be involved in tissue injury and/or mucosal defence mechanisms. Here, we studied the effects of leptin on colonic mucus secretion and rat mucin 2 (rMuc2) expression. Wistar rats ob/ob mice were used. Secretion was followed vivo perfused colon model. Mucus quantified by ELISA, rMuc2 mRNA levels real-time RT PCR. The alone or association with protein kinase C (PKC) phosphatidylinositol 3-kinase (PI3K) inhibitors secreted human mucus-secreting HT29-MTX cells...
The enterohormone glucagon-like peptide-1 (GLP-1) is required to amplify glucose-induced insulin secretion that facilitates peripheral glucose utilisation. Alteration in GLP-1 during obesity has been reported but still controversial. Due the high adaptability of intestinal cells environmental changes, we hypothesised density GLP-1-producing could be modified by nutritional factors prevent deterioration metabolic condition obesity. We quantified L-cell jejunum samples collected Roux-en-Y...
Type 1 diabetes (T1D) is an autoimmune disease caused by the destruction of pancreatic β-cells producing insulin. Both T1D patients and animal models exhibit gut microbiota mucosa alterations, although exact cause for these remains poorly understood. We investigated production key cytokines controlling integrity, abundance segmented filamentous bacteria (SFB) involved in cytokines, respective role inflammation hyperglycaemia.We used several mouse as well mice rendered hyperglycaemic without...