A5047319050- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Coastal wetland ecosystem dynamics
- Agronomic Practices and Intercropping Systems
- Photosynthetic Processes and Mechanisms
- 3D Printing in Biomedical Research
- Plant nutrient uptake and metabolism
- Plant Pathogens and Fungal Diseases
- Cancer Cells and Metastasis
- Plant Stress Responses and Tolerance
- Endoplasmic Reticulum Stress and Disease
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Plant Pathogens and Resistance
- Immune Response and Inflammation
- Plant Gene Expression Analysis
- Erythrocyte Function and Pathophysiology
- Mycorrhizal Fungi and Plant Interactions
- Digestive system and related health
- Regulation of Appetite and Obesity
- Cancer-related Molecular Pathways
- Green IT and Sustainability
Centre National de la Recherche Scientifique
2010-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université Toulouse III - Paul Sabatier
2010-2024
Université de Toulouse
2010-2023
Laboratoire des Interactions Plantes Micro-Organismes
2013-2020
Institut d'Imagerie Biomédicale
2010-2016
Ashland (United States)
2013
Laboratoire de Recherche en Sciences Végétales
2008-2010
Hôpital Bichat-Claude-Bernard
2004-2010
Université Paris Cité
2010
The mechanisms involved in the cytotoxic action of oxysterols pathogenesis atherosclerosis still remain poorly understood. Among major present oxidized low-density lipoprotein, we show here that 7-ketocholesterol (7-Kchol) induces oxidative stress and/or apoptotic events human aortic smooth muscle cells (SMCs). This specific effect 7-Kchol is mediated by a robust upregulation (threefold from basal level) Nox-4, reactive oxygen species (ROS)-generating NAD(P)H oxidase homologue. was...
Coordination of different defense pathways across cell compartments produces a fully effective innate immune response.
By combining Zinnia elegans in vitro tracheary element genomics with reverse genetics Arabidopsis, we have identified a new upstream component of secondary wall formation xylary and interfascicular fibers. Walls are thin 1 (WAT1), an Arabidopsis thaliana homolog Medicago truncatula NODULIN 21 (MtN21), encodes plant-specific, predicted integral membrane protein, is member the plant drug/metabolite exporter (P-DME) family (transporter classification number: TC 2.A.7.3). Although WAT1...
Type III effector proteins from bacterial pathogens manipulate components of host immunity to suppress defence responses and promote pathogen development. In plants, targeted by some effectors called avirulence are surveyed plant disease resistance referred as "guards". The Ralstonia solanacearum protein PopP2 triggers in Arabidopsis following its perception the RRS1-R protein. Here, we show that interacts with nucleus living cells. belongs YopJ-like family cysteine proteases, which share a...
Abstract Plant and animal pathogens inject type III effectors (T3Es) into host cells to suppress immunity promote successful infection. XopD, a T3E from Xanthomonas campestris pv vesicatoria, has been proposed bacterial growth by targeting plant transcription factors and/or regulators. Here, we show that XopD the B100 strain of X. is able target MYB30, factor positively regulates Arabidopsis thaliana defense associated cell death responses bacteria through transcriptional activation genes...
This study shows that leptin induced a rapid phosphorylation of p42/44 mitogen-activated protein kinase, an enhancement both NF-κB DNA binding and transcriptional activities, concentration-dependent increase HT-29 cell proliferation. These effects are consistent with the presence receptors on membranes. The induction growth was associated population in S G2/M phase compared control cells found G0/G1 cycle. Moreover, cyclin D1 immunoreactivity enhanced leptin-treated this essentially phase....
Abstract The current agriculture main challenge is to maintain food production while facing multiple threats such as increasing world population, temperature increase, lack of agrochemicals due health issues and uprising weeds resistant herbicides. Developing novel, alternative, safe methods hence paramount importance. Here, we show that complementary peptides (cPEPs) from any gene can be designed target specifically plant coding genes. External application synthetic increases the abundance...
Screening of 26 gut peptides for their ability to inhibit growth human colon cancer HT29-D4 cells grown in 10% fetal calf serum identified orexin-A and orexin-B as anti-growth factors. Upon addition either orexin (1 microM), suppression cell was total after 24 h >70% 48 or 72 h, with an EC(50) 5 nm peptide. Orexins did not alter proliferation but promoted apoptosis demonstrated by morphological changes shape, DNA fragmentation, chromatin condensation, cytochrome c release into cytosol,...
The superoxide-producing phagocyte NADPH oxidase consists of a membrane-bound flavocytochrome b(558), the cytosol factors p47(phox), p67(phox), p40(phox), and small GTPase Rac2, which translocate to membrane assemble active complex following neutrophil activation. Interleukin-8 (IL-8) does not activate oxidase, but potentiates oxidative burst induced by stimuli such as formyl-methionyl-leucyl-phenylalanine (fMLP) via priming mechanism. effect IL-8 on components during process has never been...
Background Senescent red blood cells (RBC) are recognized, phagocytosed and cleared by tissue macrophages. During this erythrophagocytosis (EP), RBC engulfed processed in special compartments called erythrophagosomes. We previously described that following EP, heme is rapidly degraded through the catabolic activity of oxygenase (HO). Extracted iron then either exported or stored However, cellular localization early steps processing extraction during EP remains to be clearly defined....
Wood, also called secondary xylem, is a specialized vascular tissue constituted by different cell types that undergo differentiation process involving deposition of thick, lignified walls. The mechanisms needed to control the extent lignin depending on type and stage are far from being fully understood. We found Eucalyptus transcription factor EgMYB1, which known repress biosynthesis, interacts specifically with linker histone variant, EgH1.3. This interaction enhances repression EgMYB1's...
Mutualism is of fundamental importance in ecosystems. Which factors help to keep the relationship mutually beneficial and evolutionarily successful a central question. We addressed this issue for one most significant mutualistic interactions on Earth, which associates plants leguminosae family hundreds nitrogen (N2)-fixing bacterial species. Here we analyze spatio-temporal dynamics fixers non-fixers along symbiotic process Cupriavidus taiwanensis–Mimosa pudica system. N2-fixing symbionts...
Abstract Fungal effectors (small‐secreted proteins) have long been considered as species or even subpopulation‐specific. The increasing availability of high‐quality fungal genomes and annotations has allowed the identification trans‐species trans‐genera families effectors. Two avirulence effectors, AvrLm10A AvrLm10B , Leptosphaeria maculans fungus causing stem canker oilseed rape, are members such a large family neighbouring genes, organized in divergent transcriptional orientation. Sequence...
Proteases play crucial physiological functions in all organisms by controlling the lifetime of proteins. Here, we identified an atypical protease subtilase family [SBT5.2(b)] that attenuates transcriptional activation plant defence independently its activity. The SBT5.2 gene produces two distinct transcripts encoding a canonical secreted [SBT5.2(a)] and intracellular protein [SBT5.2(b)]. Concomitant to SBT5.2(a) downregulation, SBT5.2(b) expression is induced after bacterial inoculation....
Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid long chain bases (LCBs) in plants, is known to induce a calcium-dependent programmed cell death (PCD) plants. In addition, tobacco BY-2 cells, it has been shown that DHS triggers rapid production H2O2 and nitric oxide (NO). Recently, analogy what animal field, plant cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), ubiquitous enzyme involved glycolysis, suggested fulfill other functions...
Plant immune receptors, known as NOD-like receptors (NLRs), possess unique integrated decoy domains that enable plants to attract pathogen effectors and initiate a specific response. The present study aimed create library of these (IDs) screen them with identify targets for effector virulence NLR-effector interactions. This works compiles IDs found in NLRs from seven different plant species produced 78 plasmid clones containing total 104 IDs, representing 43 distinct InterPro domains. A...
Intestinal organoids are self-organized 3-dimensional (3D) structures formed by a single layer of polarized epithelial cells. This innovative in vitro model is highly relevant to study physiology the intestinal epithelium and its role nutrition barrier function. However, this has never been developed rabbits, while it would have potential applications for biomedical veterinary research. Here, we cultured rabbit caecum with either pharmacological inhibitors (2Ki medium) or L-WRN cells...
Oomycete plant pathogens secrete effector proteins to promote disease. The damaging soilborne legume pathogen Aphanomyces euteiches harbors a specific repertoire of Small Secreted Protein effectors (AeSSPs), but their biological functions remain unknown. Here we characterize AeSSP1256. function AeSSP1256 is investigated by physiological and molecular characterization Medicago truncatula roots expressing the effector. A potential protein target identified yeast-two hybrid,...
Summary Rhizobial lipochitooligosaccharidic Nod factors (NFs), specified by nod genes, are the primary determinants of host specificity in legume–Rhizobia symbiosis. We examined nodulation ability Medicago truncatula cv Jemalong A17 and M. ssp. tricycla R108 with Sinorhizobium meliloti nodF/nodL mutant, which produces modified NFs. then applied genetic functional approaches to study basis mechanism this mutant. show that mutant can nodulate but not A17. Using genomics reverse genetics, we...
Using flow cytometry, we observed that interleukin-18 (IL-18) primed human neutrophils (PMNs) in whole blood to produce superoxide anion (O2 degrees-) response N-formyl peptide (fMLP) stimulation, whereas IL-18 alone had no significant effect. In contrast tumor necrosis factor alpha (TNF-alpha), which is a cytokine known strongly prime O2 degrees- production, did not induce either p47phox phosphorylation or its translocation from the cytosol plasma membrane. However, increased PMN...