A5028167560- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Plant Water Relations and Carbon Dynamics
- Photosynthetic Processes and Mechanisms
- Fungal Biology and Applications
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Plant Pathogens and Resistance
- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Forest Ecology and Biodiversity Studies
- Horticultural and Viticultural Research
- CRISPR and Genetic Engineering
- Plant and animal studies
Laboratoire de Recherche en Sciences Végétales
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Université Toulouse III - Paul Sabatier
2016-2025
Université de Toulouse
2016-2025
Institut National Polytechnique de Toulouse
2023
AVL (France)
2007
Collège de France
1995-1996
Eucalypts are the world's most widely planted hardwood trees. Their outstanding diversity, adaptability and growth have made them a global renewable resource of fibre energy. We sequenced assembled >94% 640-megabase genome Eucalyptus grandis. Of 36,376 predicted protein-coding genes, 34% occur in tandem duplications, largest proportion thus far plant genomes. also shows highest diversity genes for specialized metabolites such as terpenes that act chemical defence provide unique...
The mutualistic symbiosis involving Glomeromycota, a distinctive phylum of early diverging Fungi, is widely hypothesized to have promoted the evolution land plants during middle Paleozoic. These arbuscular mycorrhizal fungi (AMF) perform vital functions in phosphorus cycle that are fundamental sustainable crop plant productivity. unusual biological features AMF long fascinated evolutionary biologists. coenocytic hyphae host community hundreds nuclei and reproduce clonally through large...
Extracellular vesicles (EV) are membrane particles released by cells into their environment and considered to be key players in intercellular communication. EV produced all domains of life but limited knowledge about plants is available, although implication plant defense has been suggested. We have characterized sunflower tested whether they could interact with fungal cells. were isolated from extracellular fluids seedlings transmission electron microscopy proteomic analysis. These...
Summary Glomeromycotina is a lineage of early diverging fungi that establish arbuscular mycorrhizal ( AM ) symbiosis with land plants. Despite their major ecological role, the genetic basis obligate mutualism remains largely unknown, hindering our understanding evolution and biology. We compared genomes Glomerales Rhizophagus irregularis , diaphanus cerebriforme Diversisporales Gigaspora rosea species, together those saprotrophic Mucoromycota to identify gene families processes associated...
Arbuscular mycorrhizal (AM) fungi are a diverse group of soil (Glomeromycota) that form the most ancient mutualistic association termed AM symbiosis with majority land plants, improving their nutrition uptake and resistance to stresses. In contrast great ecological implications, knowledge molecular biological mechanisms involved is still scant, partly due limited genomic resources available. Here, we describe gene repertoire new fungus Gigaspora rosea (Diversisporales). Among 86332...
Summary The R 2 3‐ MYB family, one of the largest transcription factor families in higher plants, controls a wide variety plant‐specific processes including, notably, phenylpropanoid metabolism and secondary cell wall formation. We performed genome‐wide analysis this superfamily E ucalyptus , most planted hardwood trees world‐wide. A total 141 predicted sequences identified grandis genome sequence were subjected to comparative phylogenetic analyses with rabidopsis thaliana O ryza sativa P...
MicroRNAs (miRNAs) are transcribed as long primary transcripts (pri-miRNAs) by RNA polymerase II. Plant pri-miRNAs encode regulatory peptides called miPEPs, which specifically enhance the transcription of pri-miRNA from they originate. However, paradoxically, whereas miPEPs have been identified in different plant species, poorly conserved, raising question mechanisms underlying their specificity. To address this point, we identify and re-annotate multiple Arabidopsis thaliana order to ORF...
Xylem plays a major role in plant development and is considered part of the apoplast. Here, we studied proteome Brassica oleracea cv Bartolo compared it to cell wall another Brassicaceae, model Arabidopsis thaliana. B. was chosen because technically difficult harvest enough A. thaliana xylem sap for proteomic analysis. We whole an N-glycoproteome obtained after Concanavalin A affinity chromatography. Altogether, 189 proteins were identified by LC-MS/MS using EST cDNA sequences. predicted...
Arbuscular mycorrhizal fungi (AMF), belonging to the fungal phylum Glomeromycota, form mutualistic symbioses with roots of almost 80% land plants. The release genomic data from ubiquitous AMF Rhizophagus irregularis revealed that this species possesses a large set putative secreted proteins (RiSPs) could be major importance for establishing symbiosis. In present study, we aimed identify SPs involved in establishment AM symbiosis based on comparative gene expression analyses. We first curated...
During the last fifteen years, cell wall proteomics has become a major research field with publication of more than 50 articles describing plant proteomes. The WallProtDB database been designed as tool to facilitate inventory, interpretation data and comparisons between ( http://www.polebio.lrsv.ups-tlse.fr/WallProtDB/ ) presently contains 2170 proteins ESTs identified experimentally in 36 studies performed on 11 different species. Two criteria have be met for entering WallProtDB. First one...
Abstract The current agriculture main challenge is to maintain food production while facing multiple threats such as increasing world population, temperature increase, lack of agrochemicals due health issues and uprising weeds resistant herbicides. Developing novel, alternative, safe methods hence paramount importance. Here, we show that complementary peptides (cPEPs) from any gene can be designed target specifically plant coding genes. External application synthetic increases the abundance...
Eucalypts are the most planted hardwoods worldwide. The availability of Eucalyptus grandis genome highlighted many genes awaiting functional characterization, lagging behind because lack efficient genetic transformation protocols. In order to efficiently generate knock-out mutants study function eucalypts genes, we implemented powerful CRISPR/Cas9 gene editing technology with hairy roots system. As proofs-of-concept, targeted two wood-related genes: Cinnamoyl-CoA Reductase1 (CCR1), a key...
MicroRNAs (miRNAs) are small regulatory RNA molecules (21–24 nt) regulating the expression of target genes at post-transcriptional level, by cleaving their mRNA. Because these mainly genes, miRNAs involved crossroads several biological processes (Liu et al., 2018). transcribed as long primary transcripts (pri-miRNAs) (Xie 2005). Recent findings revealed that plant pri-miRNAs encode peptides called miRNA-encoded (miPEPs) (Lauressergues 2015; Sharma 2020). miPEPs specifically enhance...
It has been shown previously that in the chick embryo cell adhesion molecule BEN/SC1/DM-GRASP is expressed by neurons inferior olive (IO) and their terminal axonal arbors cerebellar cortex, climbing fibers (Porquié et al., 1992b). Here, new information on expression of BEN during formation olivocerebellar projection adds important notion also targets olivary axons, Purkinje cells (PCs) deep nuclear neurons. This transient, starting at E7-E8 vanishing shortly after hatching. More importantly,...
Polysaccharides make up about 75% of plant cell walls and can be broken down to produce sugar substrates (saccharification) from which a whole range products obtained, including bioethanol. Cell also contain 5-10% proteins, could used tailor them for agroindustrial uses. Here we present wall proteomics data Brachypodium distachyon, model temperate grasses. Leaves culms were analyzed during active growth at mature stage. Altogether, 559 proteins identified by LC-MS/MS bioinformatics, among...
Auxin plays a pivotal role in various plant growth and development processes, including vascular differentiation. The modulation of auxin responsiveness through the perception signaling machinery is believed to be major regulatory mechanism controlling cambium activity wood formation. To gain more insights into roles key Aux/IAA gene regulators response these we identified characterized members family genome Eucalyptus grandis, tree worldwide economic importance. We found that slightly...
Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% CW mass) and proteins (5-10%) that play major roles in plasticity during development response to environmental cues. Here, we present proteomics data Arabidopsis thaliana roots. Plants were cultivated hydroponic conditions. protein (CWP) extracts prepared analyzed two different ways order enlarge the coverage root proteome: either directly or following an affinity chromatography on combinatorial peptide ligand...
Many small peptides regulate eukaryotic cell biology. In fungi, some of these are produced after KEX2 protease activity on proteins displaying repetitions identical or nearly motifs. Following this endoprotease activity, released in the extracellular space. This type protein maturation is involved production α-type sexual pheromone Ascomycota. other cases, processing allows secreted regulating fungal wall structure acting as mycotoxins. work, we report for first time a genome-wide search...
Summary Wood production in fast‐growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation response to combined effects of these two limiting factors remain unknown. E. were submitted four combinations K‐fertilization supply. Weighted gene co‐expression network analysis MixOmics‐based co‐regulation networks used integrate xylem transcriptome, metabolome complex traits. Functional...
The lack of resolution when studying the many different ubiquitin chain types found in eukaryotic cells has been a major hurdle to our understanding their specific roles. We currently have very little insight into cellular and physiological functions Lys-63 (K63)–linked chains, although they are second most abundant forms plant cells. To overcome this problem, we developed several large-scale approaches characterize (1) E2-E3 ubiquitination machinery driving K63-linked formation (2) K63...