A5101594705- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Genetically Modified Organisms Research
- Bioenergy crop production and management
- CRISPR and Genetic Engineering
- Genetic diversity and population structure
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Transgenic Plants and Applications
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Animal Genetics and Reproduction
- Plant biochemistry and biosynthesis
- Forest ecology and management
- Plant and animal studies
- Forest Insect Ecology and Management
- Ecology and Vegetation Dynamics Studies
- Forest Management and Policy
- Insect Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Plant Diversity and Evolution
- Plant Water Relations and Carbon Dynamics
- Plant Virus Research Studies
- Tree Root and Stability Studies
Oregon State University
2016-2025
Shandong Academy of Forestry
2021
Beijing Forestry University
2021
Annoroad Gene Technology (China)
2021
Princeton Public Schools
2019
Princeton University
2019
Corvallis Environmental Center
2016-2018
Biocom
2011-2013
Michigan Technological University
2004-2012
University of Lethbridge
2003-2012
We present the results of two exploratory parsimony analyses DNA sequences from 475 and 499 species seed plants, respectively, representing all major taxonomic groups.The data are exclusively chloroplast gene rbcL, which codes for large subunit ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO or RuBPCase).We used different state-transformation assumptions resulting in sets cladograms: (i) equal-weighting 499-taxon analysis; (ii) a procedure that differentially weights transversions...
Cytosine DNA methylation is a heritable epigenetic mark present in many eukaryotic organisms. Although likely has conserved role gene silencing, the levels and patterns of appear to vary drastically among different Here we used shotgun genomic bisulfite sequencing (BS-Seq) compare eight diverse plant animal genomes. We found that are very similar flowering plants with methylated cytosines detected all sequence contexts, whereas CG predominates animals. Vertebrates have throughout genome...
Forest trees display a perennial growth behavior characterized by multiple-year delay in flowering and, temperate regions, an annual cycling between and dormancy. We show here that the CO/FT regulatory module, which controls time response to variations daylength plants, aspen trees. Unexpectedly, however, it also short-day-induced cessation bud set occurring fall. This mechanism can explain ecogenetic variation highly adaptive trait: critical for displayed sampled across latitudinal gradient...
Eucalypts are the world's most widely planted hardwood trees. Their outstanding diversity, adaptability and growth have made them a global renewable resource of fibre energy. We sequenced assembled >94% 640-megabase genome Eucalyptus grandis. Of 36,376 predicted protein-coding genes, 34% occur in tandem duplications, largest proportion thus far plant genomes. also shows highest diversity genes for specialized metabolites such as terpenes that act chemical defence provide unique...
Abstract Background Real-time reverse transcription PCR (RT-PCR) has greatly improved the ease and sensitivity of quantitative gene expression studies. However, accurate measurement with this method relies on choice a valid reference for data normalization. Studies rarely verify that levels genes are adequately consistent among samples used, nor compare alternative to assess which most reliable experimental conditions analyzed. Results Using real-time RT-PCR study 10 poplar (genus Populus )...
Annual plants grow vegetatively at early developmental stages and then transition to the reproductive stage, followed by senescence in same year. In contrast, after successive years of vegetative growth ages, woody perennial shoot meristems begin repeated transitions between sexual maturity. However, it is unknown how these occur without a conflict growth. We report that functionally diverged paralogs FLOWERING LOCUS T1 ( FT1 ) T2 FT2 ), products whole-genome duplication homologs Arabidopsis...
Abstract Epigenetic variation is likely to contribute the phenotypic plasticity and adaptative capacity of plant species, may be especially important for long‐lived organisms with complex life cycles, including forest trees. Diverse environmental stresses hybridization/polyploidization events can create reversible heritable epigenetic marks that transmitted subsequent generations as a form molecular “memory”. changes might also ability plants colonize or persist in variable environments. In...
Arthropods were the first animals to conquer land and air. They encompass more than three quarters of all described living species. This extraordinary evolutionary success is based on an astoundingly wide array highly adaptive body organizations. A lack robustly resolved phylogenetic relationships, however, currently impedes reliable reconstruction underlying processes. Here, we show that phylogenomic data can substantially advance our understanding arthropod evolution resolve several...
Trees present a life form of paramount importance for terrestrial ecosystems and human societies because their ecological structure physiological function provision energy industrial materials. The genus Populus is the internationally accepted model molecular tree biology. We have analyzed 102,019 ESTs that clustered into 11,885 clusters 12,759 singletons. also provide >4,000 assembled full clone sequences to serve as basis upcoming annotation genome sequence. A public web-based EST database...
Extensive variation in synonymous and nonsynonymous rates of substitution was observed among 50 sequences the gene coding for large subunit ribulose-1,5-bisphosphate carboxylase (rbcL) representing bryophyte, conifer, dicot, monocot taxa. Relative rate tests revealed differences up to 138% substitutions 85% ones. Within angiosperms, annual forms evolved more rapidly, on average, than perennial forms. This heterogeneity extensive at sites ones, it resulted primarily from a recent acceleration...
Abstract We identified a dwarf transgenic hybrid poplar (Populus tremula × Populus alba) after screening of 627 independent activation-tagged lines in tissue culture, greenhouse, and field environments. The cause the phenotype was hyperactivated gene encoding GA 2-oxidase (GA2ox), major gibberellin (GA) catabolic enzyme plants. mutation resulted from insertion strong transcriptional enhancer near transcription start site. Overexpression GA2ox (PtaGA2ox1) caused hyperaccumulation mRNA...
Abstract The role of gibberellins (GAs) in regulation lateral root development is poorly understood. We show that GA-deficient (35S:PcGA2ox1) and GA-insensitive (35S:rgl1) transgenic Populus exhibited increased proliferation elongation under vitro greenhouse conditions, these effects were reversed by exogenous GA treatment. In addition, RNA interference suppression two poplar 2-oxidases predominantly expressed roots also decreased formation. GAs negatively affected formation inhibiting...
Members of the CENTRORADIALIS (CEN)/TERMINAL FLOWER 1 (TFL1) subfamily control shoot meristem identity, and loss-of-function mutations in both monopodial sympodial herbaceous plants result dramatic changes plant architecture. We studied degree conservation between woody perennial system regulation by overexpression RNA interference (RNAi)-mediated suppression poplar orthologs CEN, related gene MOTHER OF FT AND TFL (MFT). Field study transgenic poplars (Populus spp.) for over 6 years showed...
Summary PTLF , the Populus trichocarpa homolog of LEAFY ( LFY ) and FLORICAULA was cloned to assess its function in a dioecious tree species. In situ hybridization studies showed that gene expressed most strongly developing inflorescences. Expression also seen leaf primordia very young leaves, notably apical vegetative buds near inflorescences, but seedlings. Although ectopic expression cDNA Arabidopsis accelerated flowering, only one many tested transgenic lines flowered precociously. The...
Abstract Transgenic down-regulation of the Pt4CL1 gene family encoding 4-coumarate:coenzyme A ligase (4CL) has been reported as a means for reducing lignin content in cell walls and increasing overall growth rates, thereby improving feedstock quality paper bioethanol production. Using hybrid poplar (Populus tremula × Populus alba), we applied this strategy examined field-grown transformants both effects on wood biochemistry tree productivity. The reductions contents obtained correlated well...
Small, volatile hydrocarbons, including trichloroethylene, vinyl chloride, carbon tetrachloride, benzene, and chloroform, are common environmental pollutants that pose serious health effects. We have developed transgenic poplar (Populus tremula x Populus alba) plants with greatly increased rates of metabolism removal these through the overexpression cytochrome P450 2E1, a key enzyme in variety halogenated compounds. The exhibited from hydroponic solution. When were exposed to gaseous they...
See also the Commentary by Lexer and Stölting
ABSTRACT We studied xylem anatomy and hydraulic architecture in 14 transgenic insertion events a control line of hybrid poplar ( Populus spp.) that varied lignin content. Transgenic had different levels down‐regulation two genes encoding 4‐coumarate:coenzyme A ligase (4CL). Two‐year‐old trees were characterized after growing either as free‐standing the field or supported by stakes greenhouse. In trees, 20 to 40% reduction content was associated with increased vulnerability embolism, shoot...
Significance Timing of vegetative bud dormancy is an environmentally and economically important trait whose importance will grow due to rapid climate changes. However, the underpinning regulatory mechanisms are still poorly understood. We report identification characterization Early Bud-Break 1 ( EBB1 ) gene in poplar that regulates timing bud-break. plays a major integrative role reactivation shoot apical meristem after winter dormancy. The knowledge about function can enable novel...