A5086958208- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Plant nutrient uptake and metabolism
- Epigenetics and DNA Methylation
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Plant responses to water stress
- Soybean genetics and cultivation
- Cassava research and cyanide
- Plant Virus Research Studies
- Light effects on plants
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Genetics, Aging, and Longevity in Model Organisms
- Plant Pathogenic Bacteria Studies
- Signaling Pathways in Disease
- Pluripotent Stem Cells Research
- Nematode management and characterization studies
- Insect and Pesticide Research
University of California, Los Angeles
2016-2025
Broad Center
2016-2025
Henan Polytechnic University
2025
Zhejiang University
2025
University of Cambridge
2023
Howard Hughes Medical Institute
2008-2023
University of Hawaii System
2022
University of Hawaiʻi at Mānoa
2022
Yale University
1996-2017
Institute of Molecular and Cell Biology
2008-2014
Cytosine DNA methylation is a heritable epigenetic mark present in many eukaryotic organisms. Although likely has conserved role gene silencing, the levels and patterns of appear to vary drastically among different Here we used shotgun genomic bisulfite sequencing (BS-Seq) compare eight diverse plant animal genomes. We found that are very similar flowering plants with methylated cytosines detected all sequence contexts, whereas CG predominates animals. Vertebrates have throughout genome...
Abstract Background 5-Hydroxymethylcytosine (5hmC) was recently found to be abundantly present in certain cell types, including embryonic stem cells. There is growing evidence that TET proteins, which convert 5-methylcytosine (5mC) 5hmC, play important biological roles. To further understand the function of an analysis genome-wide localization this mark required. Results Here, we have generated a map 5hmC human cells by hmeDIP-seq, hydroxymethyl-DNA immunoprecipitation followed massively...
In honey bees ( Apis mellifera ), the development of a larva into either queen or worker depends on differential feeding with royal jelly and involves epigenomic modifications by DNA methyltransferases. To understand role methylation in this process we sequenced larval methylomes both queens workers. We show that number differentially methylated genes (DMGs) head is significantly increased relative to adult brain (2,399 vs. 560) more than 80% DMGs up-methylated larvae. Several highly...
Transposable elements (TEs) and DNA repeats are commonly targeted by histone methylation to achieve epigenetic gene silencing. We isolated mutations in two Arabidopsis genes, AtMORC1 AtMORC6, which cause derepression of DNA-methylated genes TEs but no losses or methylation. AtMORC6 members the conserved Microrchidia (MORC) adenosine triphosphatase (ATPase) family, predicted catalyze alterations chromosome superstructure. The atmorc1 atmorc6 mutants show decondensation pericentromeric...
Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps DNA methylation in several regenerated rice lines. We found that all tested plants had significant losses compared to non-regenerated plants. Loss was largely stable across generations, and certain sites genome were particularly susceptible loss methylation. at promoters associated with...
Studies of DNA methylation from fungi, plants, and animals indicate that gene body is ancient highly conserved in eukaryotic genomes, but its role has not been clearly defined. It postulated regulation alternative splicing transcripts was an original function methylation, a direct experimental test the effect on slicing at whole genome level never performed. To do this, we developed unique method to administer RNA interference (RNAi) high-throughput noninvasive manner then used it knock down...
Abstract Pathogens rely on expression of host susceptibility ( S ) genes to promote infection and disease. As DNA methylation is an epigenetic modification that affects gene expression, blocking access through targeted could increase disease resistance. Xanthomonas phaseoli pv. manihotis , the causal agent cassava bacterial blight (CBB), uses transcription activator-like20 (TAL20) induce MeSWEET10a . In this work, we direct TAL20 effector binding element within promoter using a synthetic...
SGT1 and Rar1 are important signaling components of resistance (R) gene-mediated plant innate immune responses. Here we report that associate with the molecular chaperone Hsp90. In addition, show Hsp90 associates protein N confers to tobacco mosaic virus. This suggests Hsp90-SGT1-Rar1 R proteins might exist in one complex. Suppression Nicotiana benthamiana plants shows it plays an role growth development. suppression NN compromises N-mediated Our results reveal a new for SGT1-...
Repression of photomorphogenesis in Arabidopsis thaliana requires activity the COP9 signalosome (CSN), CDD, and COP1 complexes, but how these three complexes work concert to accomplish this important developmental switch has remained unknown. Here, we demonstrate that CULLIN4 (CUL4) associates with CDD complex a common catalytic subunit form an active E3 ubiquitin ligase both vivo vitro. The partial loss function CUL4 resulted constitutive photomorphogenic phenotype respect morphogenesis...