A5016625958- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Chromosomal and Genetic Variations
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Cassava research and cyanide
- Banana Cultivation and Research
- Insect symbiosis and bacterial influences
- Caching and Content Delivery
- Phytoplasmas and Hemiptera pathogens
- Genetically Modified Organisms Research
- Plant Gene Expression Analysis
- Cocoa and Sweet Potato Agronomy
- Plant and Fungal Interactions Research
- Pluripotent Stem Cells Research
- Innovation and Socioeconomic Development
- Genetic and Environmental Crop Studies
- Plant responses to water stress
University of California, Los Angeles
2018-2025
Agriculture and Agri-Food Canada
2020-2024
University of British Columbia
2014-2018
DNA methylation is an important epigenetic modification involved in gene regulation and transposable element silencing. Changes can be heritable and, thus, lead to the formation of stable epialleles. A well-characterized example a epiallele plants fwa, which consists loss cytosine (5mC) promoter FLOWERING WAGENINGEN (FWA) gene, causing up-regulation FWA late-flowering phenotype. Here we demonstrate that fusion between catalytic domain human demethylase TEN-ELEVEN TRANSLOCATION1 (TET1cd)...
Abstract Pathogens rely on expression of host susceptibility ( S ) genes to promote infection and disease. As DNA methylation is an epigenetic modification that affects gene expression, blocking access through targeted could increase disease resistance. Xanthomonas phaseoli pv. manihotis , the causal agent cassava bacterial blight (CBB), uses transcription activator-like20 (TAL20) induce MeSWEET10a . In this work, we direct TAL20 effector binding element within promoter using a synthetic...
The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine methylation. ability to manipulate RdDM would shed light on the mechanisms and applications of control expression. Here, we identified diverse proteins that are capable targeting silencing Arabidopsis when tethered an artificial zinc finger (ZF-RdDM). We studied their order action within by testing target different mutants. also evaluated ectopic siRNA biogenesis, RNA polymerase V (Pol V)...
Significance Site-specific modification of epigenetic marks such as DNA methylation to regulate gene expression is a unique approach enhance economically important crop traits. This allows for the maintenance introduced modifications in absence initial transgene inducer subsequent generations and relies largely on cytosines CG-specific sequence context. We have developed targeted tool based bacterial methyltransferase CRISPR-Cas9 platform directly methylate at CG sites Arabidopsis . These...
Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication movement. Utilizing this concept, several viral-based guide platforms CRISPR-Cas9 genome editing have been developed. The system has also adapted epigenome editing. While systems developed based gene activation or site-specific DNA demethylation, viral RNAs remains to be these purposes. To address gap we a tobacco rattle (TRV)-based single in Arabidopsis...
Abstract Patterning of DNA methylation in eukaryotic genomes is controlled by de novo methylation, maintenance mechanisms and demethylation pathways. In Arabidopsis thaliana , enzymes are clearly important for shaping patterns, but how they regulated poorly understood. Here we show that the targeting histone H3 lysine four trimethylation (H3K4me3) with catalytic domain SDG2 methyltransferase potently erased gene silencing at FWA also CG many other regions genome. This erasure was completely...
Cassava production in sub-Saharan Africa is severely impacted by diseases. Most pathogens require interaction with host susceptibility factors to complete their life cycles and cause disease. Targeted DNA methylation an epigenetic strategy alter gene expression plants we previously reported that a zinc-finger fused DMS3 could establish at the promoter of MeSWEET10a , bacterial gene, this resulted decreased Here, attempt similar for cassava brown streak This disease caused ipomoviruses CBSV...
Abstract Targeted genome editing by Clustered Regularly Interspaced Short Palindromic Repeat- CRISPR-associated (CRISPR-Cas) system has revolutionized basic and translational plant research. There is widespread use of CRISPR-Cas technology which the potential to address challenges like food insecurity climate crisis. Crops with improved traits (e.g., higher yield, drought tolerant) that would take several years generate can now be developed at a much reduced time, drastically expediting...
Abstract Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication movement. Utilizing this concept, several viral-based guide platforms CRISPR-Cas9 genome editing have been developed. The system has also adapted epigenome editing. While systems developed based gene activation or site-specific DNA demethylation, viral RNAs remains to be these purposes. To address gap we a tobacco rattle (TRV)-based single in...
Abstract Pathogens rely on expression of host susceptibility (S) genes to promote infection and disease. As DNA methylation is an epigenetic modification that affects gene expression, blocking access S through targeted could increase disease resistance. Xanthomonas phaseoli pv. manihotis , the causal agent cassava bacterial blight (CBB), uses transcription activator-like20 (TAL20) induce MeSWEET10a . In this work, we direct TAL20 effector binding element within promoter using a synthetic...
Plant genetic resources (PGRs) are the basis for food and nutritional security, enhanced utilization of these is paramount importance improvement crops. Till date use PGRs has been limited in conventional breeding programmes developing widely adapted crop varieties. In recent past, genome-wide association studies, genomics functional approaches using diverse germplasm accessions have facilitated discovery novel quantitative trait loci (QTLs), genes alleles associated with useful agronomic...
British Columbia (BC) is the lead producer of sweet cherries in Canada with more than 2,000 ha production and a farm gate value over CAD$100 million annually. Since 2010, an outbreak little cherry disease caused by Little virus 1 (LChV1) 2 (LChV2), as well X-disease (XD) 'Candidatus Phytoplasma pruni' has significant economic losses neighboring Washington State (WA), USA. LChV1 LChV2 have long been known to occur BC (Theilmann et al. 2002); however, 'Ca. P. not yet reported BC. Due its...