A5111168166- Nematode management and characterization studies
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Animal Genetics and Reproduction
- Plant and animal studies
- Trauma and Emergency Care Studies
- Insect and Pesticide Research
- Genetically Modified Organisms Research
- CRISPR and Genetic Engineering
- Abdominal Trauma and Injuries
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Cardiac Arrest and Resuscitation
- Plant Pathogens and Resistance
- Entomopathogenic Microorganisms in Pest Control
- Marine Biology and Ecology Research
- Emergency and Acute Care Studies
- Insect Resistance and Genetics
- Papaya Research and Applications
- Cardiac, Anesthesia and Surgical Outcomes
- Identification and Quantification in Food
- Injury Epidemiology and Prevention
- COVID-19 diagnosis using AI
- Insect and Arachnid Ecology and Behavior
Canadian Food Inspection Agency
2008-2024
University of Waterloo
2024
Legacy Emanuel Medical Center
2021
Scripps Mercy Hospital
2021
Madigan Army Medical Center
2021
Orthopaedic Trauma Association
2021
University of Colorado Denver
2020
Denver Health Medical Center
2020
University of Colorado Anschutz Medical Campus
2020
Children's Hospital Colorado
2020
The yellow potato cyst nematode, Globodera rostochiensis, is a devastating plant pathogen of global economic importance. This biotrophic parasite secretes effectors from pharyngeal glands, some which were acquired by horizontal gene transfer, to manipulate host processes and promote parasitism. G. rostochiensis classified into pathotypes with different resistance-breaking phenotypes. We generate high quality genome assembly for pathotype Ro1, identify putative transfer events, map expression...
Monitoring the environment for pollution, pesticides, and pathogens is crucial protecting human, agriculture, overall ecosystem health. Diverse strategies ranging from physical sensors to sentinel species have been used environmental monitoring. The European honey bee, Apis mellifera, a globally managed pollinator that can serve as continuous biomonitoring species. During foraging, bees are exposed contaminants carry them their hives where they be detected quantified. Although individual...
Apple rubbery wood is a disease of apple found around the world, often associated with flat limb disease, and regulated in many countries. Despite its long history cultivation, disease's causal agent has remained elusive. In this study, next-generation sequencing (NGS) was used to identify characterize several related novel viral agents from wood-infected plants, which have been named virus (ARWV) 1 2. Additional specimens tested positive by polymerase chain reaction primers designed ARWV 2...
BACKGROUND Although several trauma centers have developed direct to operating room (DOR) resuscitation programs, there is little published data on optimal patient selection, practices, and outcomes. We sought analyze triage criteria interventions associated with DOR outcomes resource utilization. METHODS Retrospective review of all adult resuscitations for a 6-year period was performed. Triage were analyzed individually grouped into categories: mechanism, physiology, anatomy/injury, or...
Abstract A real-time PCR polymerase chain reaction (real-time PCR) method was developed to detect and differentiate Bursaphelenchus xylophilus (pine wood nematode, PWN) from other wood-inhabiting nematode species. primer set a specific Taqman® fluorescent probe were designed amplify target B. heat shock protein 70 sequences. After optimization, this assay shown be highly sensitive, detecting at least 0.005 ng of genomic DNA, as well DNA extracted single nematodes. The practical application...
Healthy agroecosystems are dependent on a complex web of factors and inter-species interactions. Flowers hubs for pathogen transmission, including the horizontal or vertical transmission plant-viruses bee-viruses. Pollination by European honey bee (Apis mellifera) is critical industrial fruit production, but bees can also vector viruses other pathogens between individuals. Here, we utilized commercial pollination services in blueberry (Vaccinium corymbosum) farms metagenomics-based plant...
Transgenic soybean line GTS-40-3-2, marketed under the trade name Roundup Ready (RR) soy, was developed by Monsanto (USA) to allow for use of glyphosate, active ingredient herbicide Roundup, as a weed control agent. RR soy first approved in Canada environmental release and feed products 1995 later food 1996 is widely grown Canada. Consumer concern issues have resulted proposed labeling regulations foods derived from genetically engineered crops. One requirement ability detect accurately...
A rapid method was developed for concurrent screening of transgenic elements in GM canola. This utilizes a single multiplex PCR coupled with an oligonucleotide DNA array capable simultaneously detecting the 12 approved canola lines Canada. The assay includes construct-specific identification lines, common (e.g., CaMV 35S promoter, Agrobacterium tumefaciens nos terminator, or nptII gene) unapproved canola-specific endogenous gene, and genes from heterologous crops to serve as additional...
BACKGROUND Direct-to-operating room (DOR) resuscitation expedites interventions for trauma patients. Perceived benefit from the surgeon's perspective is not well known. This study assesses integration of a real-time surgeon assessment tool into DOR protocol. METHODS Surgeon results prospective cases were analyzed. Analysis assessed patient factors and perception appropriateness DOR. Multivariate analysis identified independent associated with perceived benefit. RESULTS A total 104 patients...
Abstract A molecular diagnostic method has been designed for the detection and identification of Bursaphelenchus xylophilus. Heat shock protein 70 gene sequences from B. xylophilus closely related mucronatus were compared used to design primers Bx701F Bx701R which amplify a 171 base pair fragment by PCR. As control, Bm701F Bm701R specifically 168 mucronatus. After optimisation, shown be highly sensitive could easily detect 23 target copies, or less than one nematode. Species-specific was...
The potato cyst nematode (PCN), Globodera rostochiensis, has been present in Central Saanich on Vancouver Island for at least 45 years. Eradication/control efforts have ongoing, with regulations enacted the early 1980s restricting planting of host crops and movement soil. Surveys monitoring populations minimal since place only one limited study 1990s. In this report, a survey eight fields was undertaken, chosen as most likely sites that may still harbor viable PCN cysts. Conventional...
Several countries have introduced mandatory labeling requirements on foods derived from genetically modified organisms. Real-time quantitative Polymerase Chain Reaction (PCR) has quickly become the method of choice in support these regulations and requires development separate PCR assays targeting transgenic sequence as well a specific endogenous gene sequence. To develop Brassica napus-specific assay, partial sequences acetyl-CoA carboxylase BnACCg8 B. napus closely related rapa were...
Abstract Two Canadian isolates of the potato cyst nematode (PCN), Globodera rostochiensis pathotype Ro1, were evaluated for their ability to replicate on several species nightshade. Nightshade is commonly found in potato-growing fields and can potentially act as an alternative host, thereby interfering with eradication/control measures PCN. Accessions Solanum carolinense, S. americanum, dulcamara, ptychanthum, rostratum, sarrachoides, sisymbriifolium, S physalifolium, triflorum, villosum...
Wilderness activities expose outdoor enthusiasts to austere environments with injury potential, including falls from height. The majority of published data on while climbing or hiking are emergency departments. We sought more accurately describe the pattern wilderness that lead serious requiring trauma center evaluation and further distinguish as a unique injury.Data were collected 17 centers in 11 states all (fall cliff: International Classification Diseases, Ninth Revision, e884.1; 10th...
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Plant viral infections cause significant economic losses, totalling $350 billion USD in 2021. With no treatment for virus-infected plants, accurate and efficient diagnosis is crucial to preventing controlling these diseases. High-throughput sequencing (HTS) enables cost-efficient identification of known unknown viruses. However, existing diagnostic pipelines face challenges. First, many methods depend on subjectively chosen parameter values, undermining their robustness across various data...
Commercially cultivated Prunus species are commonly grown in adjacent or mixed orchards, and can be infected with unique shared viruses. Apple (Malus domestica), another member of the Rosacea distantly related to Prunus, share same growing regions common pathogens. Pollen a major route for virus transmission, analysis pollen virome tree fruit orchards provide insights into these pathogen complexes from production sites. Commercial honey bee (Apis mellifera) pollination is essential improved...
The extraction of double stranded (ds) RNA is a common enrichment method for the study, characterization, and detection viruses. In addition to viruses, viroids, some DNA can also be detected from dsRNA enriched extracts which makes it an attractive detecting wide range viruses when coupled with HTS. Several strategies have been developed. oldest utilizes selective binding properties cellulose. More recent methods are based on application anti-dsRNA antibodies viral proteins specific...