A5082947493- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Genetics, Aging, and Longevity in Model Organisms
- Agronomic Practices and Intercropping Systems
- Phytase and its Applications
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Parasite Biology and Host Interactions
- Transgenic Plants and Applications
- Polysaccharides and Plant Cell Walls
- Banana Cultivation and Research
- Insect symbiosis and bacterial influences
- Quantum Dots Synthesis And Properties
- Genetically Modified Organisms Research
- Insect and Arachnid Ecology and Behavior
- Genomics, phytochemicals, and oxidative stress
- Insect-Plant Interactions and Control
- Mycorrhizal Fungi and Plant Interactions
University of Leeds
2014-2024
Planta
2014-2016
University of Westminster
2000-2014
Newcastle University
1996
De Montfort University
1995
Rothamsted Research
1988-1992
Durham University
1991
Abstract In field conditions, plants may experience numerous environmental stresses at any one time. Research suggests that the plant response to multiple is different from for individual stresses, producing nonadditive effects. particular, molecular signaling pathways controlling biotic and abiotic stress responses interact antagonize another. The transcriptome of Arabidopsis (Arabidopsis thaliana) concurrent water deficit (abiotic stress) infection with plant-parasitic nematode Heterodera...
Abstract Background Globodera pallida is a devastating pathogen of potato crops, making it one the most economically important plant parasitic nematodes. It also an model for biology cyst Cyst nematodes and root-knot are two nematode groups together represent global threat to food security. Results We present complete genome sequence G. , with transcriptomic data from life cycle, particularly focusing on cycle stages involved in root invasion establishment biotrophic feeding site. Despite...
The yellow potato cyst nematode, Globodera rostochiensis, is a devastating plant pathogen of global economic importance. This biotrophic parasite secretes effectors from pharyngeal glands, some which were acquired by horizontal gene transfer, to manipulate host processes and promote parasitism. G. rostochiensis classified into pathotypes with different resistance-breaking phenotypes. We generate high quality genome assembly for pathotype Ro1, identify putative transfer events, map expression...
RNA interference is of value in determining gene function many organisms. Plant parasitic nematodes are refractory to microinjection as a means introducing and do not show any oral uptake until they within plants. We have used octopamine stimulate by prepara-sitic second stage juveniles two cyst nematodes, Heterodera glycines Globodera pallida. This new technique was facilitate double stranded (dsRNA) together with fluoroscein isothiocyanate visual marker. Targeting cysteine proteinases did...
The cysteine proteinase inhibitor, oryzacystatin‐I (Oc‐I), and several engineered Oc‐I variants have been tested for efficacy in inhibiting growth development of both the free‐living nematode, Caenorhabditis elegans , plant parasitic nematode Globodera pallida . To assist design protein engineering experiments to improve Oc‐I, an alignment 28 cystatins a molecular model were generated. Inhibitory activities ( K i ) wild‐type variant forms against papain C. proteinase, gcp‐1, measured. For...
Plant nematodes are major pests of agriculture. Transgenic plant technology has been developed based on the use proteinase inhibitors as nematode anti‐feedants. The approach offers prospects for novel resistance and reduced environmentally damaging nematicides. A modified rice cystatin, Oc‐IΔD86, expressed a transgene in Arabidopsis thaliana , profound effect size fecundity females both Heterodera schachtii (beet‐cyst nematode) Meloidogyne incognita (root‐knot nematode). No either species...
Abstract Arbuscular mycorrhizal (AM) fungi associate with the roots of many plant species, enhancing their hosts access to soil nutrients whilst obtaining carbon supply directly as photosynthates. AM often face competition for from other organisms. The mechanisms by which plants prioritise allocation mutualistic over parasitic symbionts remain poorly understood. Here, we show that host potato ( Solanum tuberosum cv. Désirée) selectively allocate resources tissues interacting rather than...
The pea (Pisum sativum L.) gene PSMTA has an ORF encoding a predicted protein with sequence similarity to class I metallothioneins (MTS). To examine the metal-binding properties of it been expressed in E. coli as carboxyterminal extension glutathione-S-transferase (GST). Metal ions were associated when purified from lysates grown metal supplemented media. pH half-dissociation Zn, Cd and Cu recombinant fusion was determined be 5.35, 3.95 1.45 respectively, compared equivalent estimates 4.50,...
Changes in transcript abundance of genes expressed the three pharyngeal gland cells Heterodera glycines after host invasion were monitored by quantitative polymerase chain reaction (qPCR) and consequences disrupting their expression studied RNAi treatment prior to invasion. Two transcripts known be two subventral (hg-pel hg-eng-1), a further single dorsal cell only (hg-gp hg-syv46), fifth (hg-cm) was both types. The qPCR study established that hg-syv46 hg-gp increased 2 days postinfection...
Induction of abiotic stress in tomato plants has been proposed as a mechanism for improving the nutritional quality fruits. However, occurrence biotic can interfere with normal responses. In this study, combined effect water and infection plant-parasitic nematodes on was investigated. Plants were exposed to one or both stresses, levels phenolic compounds, carotenoids, sugars fruits analyzed well physiological Levels carotenoids lycopene β-carotene lower water-stressed tomatoes but exhibited...
The effects of brassica green manures on Globodera pallida were assessed in vitro and soil microcosms. Twelve 22 accessions significantly inhibited the motility G. infective juveniles vitro. Green selected brassicas then incorporated into containing encysted eggs pallida. Their effect egg viability was estimated by quantifying nematode actin 1 mRNA RT-qPCR. leaf glucosinolate profiles plants determined high-performance liquid chromatography. Three Brassica juncea lines (Nemfix, Fumus,...
Plant pathogens and parasites are a major threat to global food security. parasitism has arisen four times independently within the phylum Nematoda, resulting in at least one parasite of every crop world. Some species most economically important order (Tylenchida) secrete proteins termed effectors into their host during infection re-programme development immunity. The precise detail how nematodes evolve new is not clear. Here we reconstruct evolutionary history novel effector gene family. We...
Sedentary endoparasitic nematodes are obligate biotrophs that modify host root tissues, using a suite of effector proteins to create and maintain feeding site is their sole source nutrition. Using assumptions about the characteristics genes involved in plant-nematode biotrophic interactions inform identification strategy, we provide description characterisation novel group hyper-variable extracellular effectors termed HYP, from potato cyst nematode Globodera pallida. HYP comprise large gene...
The responsiveness of the cauliflower mosaic virus 35S promoter in feeding sites developed by both sexes Heterodera schachtii and female Meloidogyne incognita has been studied. objective was to establish value green-fluorescent protein (GFP) as a nondestructive reporter gene system for characterizing activity at nematode vivo. Growth units were devised that allowed individual roots Arabidopsis thaliana be observed bright-field epifluorescent illumination. Changes GFP expression visually...
SUMMARY Taxonomy: Superkingdom Eukaryota; kingdom Metazoa; phylum Nematoda; class Chromadorea; order Tylenchida; suborder Tylenchina; superfamily Tylenchoidea; family Heteroderidae; subfamily Heteroderinae; main genera Heterodera and Globodera.Cyst nematodes comprise approximately 100 known species in six genera. They are pathogens of temperate, subtropical tropical plant the host range many is narrow. The most economically important within Globodera pallida G. rostochiensis potato crops....
SUMMARY RNA interference (RNAi), first described for Caenorhabditis elegans , has emerged as a powerful gene silencing tool investigating function in range of organisms. Recent studies have its application to plant parasitic nematodes. Genes expressed cell types are silenced when preparasitic juvenile nematodes take up double‐stranded (ds)RNA that elicits systemic RNAi response. Important developments over the last year shown planta expression dsRNA targeting nematode can successfully induce...