A5051406444- Malaria Research and Control
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Genomics and Phylogenetic Studies
- Invertebrate Immune Response Mechanisms
- Complement system in diseases
- Bioinformatics and Genomic Networks
- Parasite Biology and Host Interactions
- Parasitic Infections and Diagnostics
- Trypanosoma species research and implications
- Helminth infection and control
- Aquaculture disease management and microbiota
- Machine Learning in Bioinformatics
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Nematode management and characterization studies
- Coccidia and coccidiosis research
- Insect symbiosis and bacterial influences
- Global Maternal and Child Health
- Child Nutrition and Water Access
- vaccines and immunoinformatics approaches
- Legume Nitrogen Fixing Symbiosis
- Vector-borne infectious diseases
- Cancer-related molecular mechanisms research
- Toxoplasma gondii Research Studies
The Gurdon Institute
2021-2025
University of Cambridge
2021-2025
Wellcome Trust
2014-2024
Wellcome Sanger Institute
2014-2023
University of Minho
2022
Columbia University Irving Medical Center
2022
ORCID
2022
Genomics (United Kingdom)
2014-2020
Médecins Sans Frontières
2013
University College London
2007-2012
Parasitic nematodes (roundworms) and platyhelminths (flatworms) cause debilitating chronic infections of humans animals, decimate crop production are a major impediment to socioeconomic development. Here we report broad comparative study 81 genomes parasitic non-parasitic worms. We have identified gene family births hundreds expanded families at key nodes in the phylogeny that relevant parasitism. Examples include modulate host immune responses, enable parasite migration though tissues or...
Bursaphelenchus xylophilus is the nematode responsible for a devastating epidemic of pine wilt disease in Asia and Europe, represents recent, independent origin plant parasitism nematodes, ecologically taxonomically distinct from other nematodes which genomic data available. As well as being an important pathogen, B. genome thus provides unique opportunity to study evolution mechanism parasitism. Here, we present high-quality draft sequence inbred line xylophilus, use this investigate...
The small ruminant parasite Haemonchus contortus is the most widely used parasitic nematode in drug discovery, vaccine development and anthelmintic resistance research. Its remarkable propensity to develop threatens viability of sheep industry many regions world provides a cautionary example effect mass administration control nematodes. phylogenetic position makes it particularly well placed for comparison with free-living Caenorhabditis elegans economically important parasites livestock humans.
Abstract Background Globodera pallida is a devastating pathogen of potato crops, making it one the most economically important plant parasitic nematodes. It also an model for biology cyst Cyst nematodes and root-knot are two nematode groups together represent global threat to food security. Results We present complete genome sequence G. , with transcriptomic data from life cycle, particularly focusing on cycle stages involved in root invasion establishment biotrophic feeding site. Despite...
Malaria parasites adopt a remarkable variety of morphological life stages as they transition through multiple mammalian host and mosquito vector environments. We profiled the single-cell transcriptomes thousands individual parasites, deriving first high-resolution transcriptional atlas entire
Taisei Kikuchi, Mark Viney, Matthew Berriman and colleagues report the genome sequences of six species nematodes from Strongyloides clade nematodes, including human animal pathogens, facultative parasites a free-living species. They find that expansions astacin SCP/TAPS gene families are associated with parasitism in these Soil-transmitted genus, cause one most prevalent neglected tropical diseases. Here we compare genomes four species, pathogen stercoralis, their close relatives...
Toxoplasma gondii is a zoonotic protozoan parasite which infects nearly one third of the human population and found in an extraordinary range vertebrate hosts. Its epidemiology depends heavily on horizontal transmission, especially between rodents its definitive host, cat. Neospora caninum recently discovered close relative Toxoplasma, whose host dog. Both species are tissue-dwelling Coccidia members phylum Apicomplexa; they share many common features, but neither humans nor shares same wide...
Global production of chickens has trebled in the past two decades and they are now most important source dietary animal protein worldwide. Chickens subject to many infectious diseases that reduce their performance productivity. Coccidiosis, caused by apicomplexan protozoa genus Eimeria , is one poultry diseases. Understanding biology parasites underpins development new drugs vaccines needed improve global food security. We have produced annotated genome sequences all seven species infect...
Single-cell RNA-sequencing is revolutionising our understanding of seemingly homogeneous cell populations but has not yet been widely applied to single-celled organisms. Transcriptional variation in unicellular malaria parasites from the Plasmodium genus associated with critical phenotypes including red blood invasion and immune evasion, transcriptional at an individual parasite level examined depth. Here, we describe adaptation a single-cell (scRNA-seq) protocol deconvolute for more than...
Elucidation of the evolutionary history and interrelatedness Plasmodium species that infect humans has been hampered by a lack genetic information for three human-infective species: P. malariae two ovale (P. o. curtisi wallikeri). These are prevalent across most regions in which malaria is endemic often undetectable light microscopy, rendering their study human populations difficult. The exact relationship these to other contested. Using new reference genome manually curated draft genome, we...
We report the latest release (version 3.0) of CATH protein domain database (http://www.cathdb.info). There has been a 20% increase in number structural domains classified CATH, up to 86 151 domains. Release 3.0 comprises 1110 fold groups and 2147 homologous superfamilies. To cope with increases diverse homologues being determined by genomics initiatives, more sensitive methods have developed for identifying boundaries multi-domain proteins recognising homologues. The classification update is...
Whipworms are common soil-transmitted helminths that cause debilitating chronic infections in man. These nematodes only distantly related to Caenorhabditis elegans and have evolved occupy an unusual niche, tunneling through epithelial cells of the large intestine. We report here whole-genome sequences human-infective Trichuris trichiura mouse laboratory model muris. On basis whole-transcriptome analyses, we identify many genes expressed a sex- or life stage-specific manner characterize...
Background: Although thousands of clinical isolates Plasmodium falciparum are being sequenced and analysed by short read technology, the data do not resolve highly variable subtelomeric regions genomes that contain polymorphic gene families involved in immune evasion pathogenesis. There is also no current standard definition boundaries these regions. Methods: Using long-read sequence (Pacific Biosciences SMRT technology), we assembled annotated 15 P. isolates, ten which newly cultured...
Babesia spp. are tick-borne, intraerythrocytic hemoparasites that use antigenic variation to resist host immunity, through sequential modification of the parasite-derived variant erythrocyte surface antigen (VESA) expressed on infected red blood cell surface. We identified genomic processes driving diversity in genes encoding VESA (ves1) comparative analysis within and between three species, (B. bigemina, B. divergens bovis). Ves1 structure diverges rapidly after speciation, notably...
Significance Sixty billion chickens are produced worldwide each year, and all at risk from Eimeria , parasites that cause coccidiosis. Control relies widely on chemoprophylaxis, but pressure to reduce drug use in farming urges development of cost-effective vaccines. Antigens such as apical membrane antigen 1 (AMA1) offer promise anticoccidial vaccine candidates, experience with related apicomplexans Plasmodium which pre-existing antigenic diversity incompatible population structure have...
Abstract Malaria parasites have a complex life cycle featuring diverse developmental strategies, each uniquely adapted to navigate specific host environments. Here we use single-cell transcriptomics illuminate gene usage across the transmission of most virulent agent human malaria - Plasmodium falciparum . We reveal trajectories associated with colonization mosquito midgut and salivary glands elucidate transcriptional signatures transmissible stage. Additionally, identify both conserved...
The neonatal mouse cerebellum shows remarkable regenerative potential upon injury at birth, wherein a subset of Nestin-expressing progenitors (NEPs) undergoes adaptive reprogramming to replenish granule cell that die. Here, we investigate how the microenvironment injured changes and contributes normally gliogenic-NEPs their reprogramming. Single transcriptomic bulk chromatin accessibility analyses NEPs from cerebella compared controls show temporary increase in cellular processes involved...
The neonatal mouse cerebellum shows remarkable regenerative potential upon injury at birth, wherein a subset of Nestin-expressing progenitors (NEPs) undergoes adaptive reprogramming to replenish granule cell that die. Here, we investigate how the microenvironment injured changes and contributes normally gliogenic-NEPs their reprogramming. Single transcriptomic bulk chromatin accessibility analyses NEPs from cerebella compared controls show temporary increase in cellular processes involved...
The potato cyst nematode Globodera pallida has biotrophic interactions with its host. induces a feeding structure - the syncytium which it keeps alive for duration of life cycle and on depends all nutrients required to develop adult stage. Interactions G. host are mediated by effectors, produced in two sets gland cells. These effectors suppress defences, facilitate migration induce formation syncytium.The recent completion genome sequence allowed us identify effector complement from this...
There are over 200 million reported cases of malaria each year, and most children living in endemic areas will experience multiple episodes clinical disease before puberty. We set out to understand how frequent malaria, which elicits a strong inflammatory response, affects the immune system whether these modifications observable absence detectable parasitaemia. used multi-dimensional approach comprising whole blood transcriptomic, cellular plasma cytokine analyses on cohort with but...
Whipworms are large metazoan parasites that inhabit multi-intracellular epithelial tunnels in the intestine of their hosts, causing chronic disease humans and other mammals. How first-stage larvae invade host epithelia establish infection remains unclear. Here we investigate early events using both Trichuris muris infections mice murine caecaloids, first in-vitro system for whipworm organoid model live helminths. We show degrade mucus layers to access cells. In syncytial tunnels, completely...
Abstract Maternal inheritance of mitochondrial DNA (mtDNA) is highly conserved in metazoans. While many species eliminate paternal mtDNA during late sperm development to foster maternal inheritance, the regulatory mechanisms governing this process remain elusive. Through a forward genetic screen Drosophila , we identified 47 mutant lines exhibiting substantial retention mature sperm. We mapped one line poldip2 gene predominantly expressed testis. Disruption led and subsequent transmission...