A5003437563- Mosquito-borne diseases and control
- Malaria Research and Control
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Heme Oxygenase-1 and Carbon Monoxide
- HIV Research and Treatment
- Insect Pest Control Strategies
- Toxoplasma gondii Research Studies
- Immunotherapy and Immune Responses
- Parasite Biology and Host Interactions
- Salmonella and Campylobacter epidemiology
- Cancer Research and Treatments
- Herpesvirus Infections and Treatments
- Insect Resistance and Genetics
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Lipid Membrane Structure and Behavior
- Redox biology and oxidative stress
Imperial College London
2013-2022
Jenner Institute
2013-2021
University of Oxford
2013-2021
The London College
2013
Abstract Malaria parasites have a complex life cycle featuring diverse developmental strategies, each uniquely adapted to navigate specific host environments. Here we use single-cell transcriptomics illuminate gene usage across the transmission of most virulent agent human malaria - Plasmodium falciparum . We reveal trajectories associated with colonization mosquito midgut and salivary glands elucidate transcriptional signatures transmissible stage. Additionally, identify both conserved...
Chikungunya virus (CHIKV) has caused extensive outbreaks in several countries within the Americas, Asia, Oceanic/Pacific Islands, and Europe. In humans, CHIKV infections cause a debilitating disease with acute febrile illness long-term polyarthralgia. Acute chronic symptoms impose major economic burden to health systems contribute poverty affected countries. An efficacious vaccine would be an important step towards decreasing by infection. Despite no licensed is yet available for CHIKV,...
Vivax malaria remains one of the most serious and neglected tropical diseases, with 132 to 391 million clinical cases per year 2.5 billion people at risk infection. A vaccine against Plasmodium vivax could have more impact than any other intervention, use a targeting multiple antigens may result in higher efficacy sporozoite infection single antigen.
Abstract Despite an efficacious prophylactic human papillomavirus (HPV) vaccine there is still a considerable global burden of HPV-related disease. Therapeutic vaccines that could prevent cancers in at-risk women are urgently needed. Most candidate therapeutic have focused on two high-risk (hr) HPV genotypes, 16 and 18, viral targets, E6 E7, which may limit coverage efficacy. We designed the synthetic gene ‘5GHPV3′ by selecting conserved regions from each six early proteins generating...
Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise detect arbovirus and malaria in the laboratory but not been evaluated field conditions. Here we investigate ability NIRS identify Plasmodium falciparum Anopheles coluzzii mosquitoes. models trained on laboratory-reared mosquitoes infected with wild parasites can parasite comparable moderate...
Salmonella enterica infections remain a challenging health issue, causing significant morbidity and mortality worldwide. Current vaccines against typhoid fever display moderate efficacy whilst no licensed are available for paratyphoid or invasive non-typhoidal salmonellosis. Therefore, there is an urgent need to develop high broad-spectrum that can protect typhoidal Salmonella. The outer membrane porins OmpC OmpF, have been shown be highly immunogenic antigens, efficiently eliciting...
The ability of an organism to sense and respond environmental redox fluctuations relies on a signaling network that is incompletely understood in apicomplexan parasites such as Toxoplasma gondii . impact changes upon the development this intracellular parasite not known. Here, we provide revised collection 58 genes containing domains related canonical antioxidant function, with their encoded proteins widely dispersed throughout different cellular compartments. We demonstrate addition...
Infectious diseases continue to pose a substantial burden on global populations, requiring innovative broad-spectrum prophylactic and treatment alternatives. Here, we have designed modular synthetic polymer nanoparticles that mimic functional components of host cell membranes, yielding multivalent nanomimics act by directly binding varied pathogens. Nanomimic blood circulation time was prolonged reformulating polymer–lipid hybrids. Femtomolar concentrations the were sufficient inhibit herpes...
Anti-bacterial proteins in mosquitoes are known to play an important modulatory role on immune responses infections with human pathogens including malaria parasites. In this study we characterized two members of the Anopheles gambiae Nimrod superfamily, namely AgNimB2 and AgEater. We confirm that current annotation An. genome incorrectly identifies AgEater as a single gene, AGAP009762. Through silico experimental approaches, it has been shown is secreted protein mediates phagocytosis...
Abstract Malaria parasites have a complex life cycle featuring diverse developmental strategies, each uniquely adapted to navigate specific host environments. Here we use single-cell transcriptomics illuminate gene usage across the transmission of most virulent agent human malaria – Plasmodium falciparum . We reveal trajectories associated with colonisation mosquito midgut and salivary glands elucidate transcriptional signatures transmissible stage. Additionally, identify both conserved...
Progress towards a protective vaccine against malaria remains slow. To date, only limited protection has been routinely achieved following immunisation with either whole-parasite (sporozoite) or subunit-based vaccines. One major roadblock to progress, and pre-erythrocytic parasite biology in general, is the continued reliance on manual salivary gland dissection for sporozoite isolation from infected mosquitoes. Here, we report development of multi-step method, based batch processing...
Abstract Complete protection against human malaria challenge has been achieved using infected mosquitoes as the delivery route for immunization with Plasmodium parasites. Strategies seeking to replicate this efficacy either a manufactured whole-parasite or subunit vaccine, however, have shown only limited success. A major roadblock whole parasite vaccine progress and understanding of infective sporozoite form in general, is reliance on manual dissection isolation from mosquitoes. We report...
Abstract The ability of an organism to sense and respond environmental redox fluctuations relies on a signaling network that is incompletely understood in apicomplexan parasites such as Toxoplasma gondii . impact changes upon the development this intracellular parasite not known. Here, we provide revised collection 58 genes containing domains related canonical antioxidant function, with their encoded proteins widely dispersed throughout different cellular compartments. We demonstrate...
Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise detect arbovirus and malaria in the laboratory but not been evaluated field conditions. Here we investigate ability NIRS identify Plasmodium falciparum Anopheles coluzzii mosquitoes. models trained on laboratory-reared mosquitoes infected with wild parasites can parasite comparable moderate...