A5047433337- Mosquito-borne diseases and control
- Malaria Research and Control
- Complement system in diseases
- Invertebrate Immune Response Mechanisms
- Trypanosoma species research and implications
- Toxoplasma gondii Research Studies
- Single-cell and spatial transcriptomics
- Photoreceptor and optogenetics research
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Neuroinflammation and Neurodegeneration Mechanisms
- Photosynthetic Processes and Mechanisms
- Fractal and DNA sequence analysis
- Veterinary medicine and infectious diseases
- Hemoglobinopathies and Related Disorders
Harvard University
2024
Walter and Eliza Hall Institute of Medical Research
2019-2022
Wellcome Sanger Institute
2019-2021
Malaria parasites adopt a remarkable variety of morphological life stages as they transition through multiple mammalian host and mosquito vector environments. We profiled the single-cell transcriptomes thousands individual parasites, deriving first high-resolution transcriptional atlas entire
Plasmodium vivax is responsible for the majority of malaria cases outside Africa. Unlike P . falciparum , life-cycle includes a dormant liver stage, hypnozoite, which can cause infection in absence mosquito transmission. An effective vaccine against blood stages would limit symptoms and pathology from such recurrent infections, therefore could play critical role control this species. Vaccine development however, lags considerably behind has many identified targets with several having...
Plasmodium vivax causes the majority of malaria outside Africa, but is poorly understood at a cellular level partly due to technical difficulties in maintaining it vitro culture conditions. In past decades, drug resistant P. parasites have emerged, mainly Southeast Asia, while some molecular markers resistance been identified, none so far confirmed experimentally, which limits interpretation markers, and hence our ability monitor control spread resistance. Some these potential identified...
Malaria parasites adopt a remarkable variety of morphological life stages as they transition through multiple mammalian host and mosquito vector environments. Here we profile the single-cell transcriptomes thousands individual parasites, deriving first high-resolution transcriptional atlas entire Plasmodium berghei cycle. We then use our to precisely define developmental single cells from three different human malaria parasite species, including isolated directly infected individuals. The...
The human malaria parasite,
This immunofluorescence assay protocol is based on the work of Tonkin et al. (2004). We updated their method and used a 96-well plate format allowing medium-throughput method.
This is a recipe for media culturing P. knowlesi in vitro. The essentially identical to that used Moon et al. (2013), with the exception we tend use horse serum rather than human serum. resulting similar routine falciparum culture, but contains both and albumax components.
This protocol is based on that described in Moon et al. (2013): https://www.pnas.org/content/110/2/531
This protocol was developed to study protein expression in Plasmodium red blood stage. Large multispanning membrane proteins, such as pvmdr1 (PVX_080100), could be extracted and detected using this method.
This drug assay was adapted from van Schalkwyk et al. (2017). In this protocol, we take as an example where chloroquine and mefloquine sensitivity are tested for 6 different P. knowlesi strains. order to faciliate calculations, provide spreadsheets that you can edit adapt your purposes.
ABSTRACT Plasmodium vivax is responsible for the majority of malaria cases outside Africa. Unlike P. falciparum , life-cycle includes a dormant liver stage, hypnozoite, which can cause infection in absence mosquito transmission. An effective vaccine against blood stages would limit symptoms and pathology from such recurrent infections, therefore could play critical role control this species. Vaccine development however, lags considerably behind has many identified targets with several having...
Abstract C-mannosylation stabilizes proteins bearing a thrombospondin repeat (TSR) domain in metazoans. Here we show that Plasmodium falciparum expresses DPY19 C-mannosyltransferase the endoplasmic reticulum and -deficiency abolishes C-glycosylation, destabilizes members of TRAP adhesin family inhibits transmission to mosquitoes. P. gametogenesis was imaged its entirety four dimensions using lattice light-sheet microscopy. This revealed defects egress exflagellation for D microgametes. While...