A5057972482- Malaria Research and Control
- Mosquito-borne diseases and control
- Inflammatory mediators and NSAID effects
- Osteoarthritis Treatment and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Trypanosoma species research and implications
- Cancer-related molecular mechanisms research
- Drug Transport and Resistance Mechanisms
- Complement system in diseases
- Platelet Disorders and Treatments
- Advanced Proteomics Techniques and Applications
- Blood groups and transfusion
- Research on Leishmaniasis Studies
- Chemokine receptors and signaling
- Photosynthetic Processes and Mechanisms
- HIV/AIDS drug development and treatment
- Hemoglobinopathies and Related Disorders
- Reproductive tract infections research
- HIV Research and Treatment
- Reproductive Physiology in Livestock
- Parkinson's Disease Mechanisms and Treatments
- Veterinary medicine and infectious diseases
- Photoreceptor and optogenetics research
- Fractal and DNA sequence analysis
Wellcome Sanger Institute
2016-2025
Wellcome Centre for Anti-Infectives Research
2024
University of Dundee
2024
Emory University
2024
University of Sheffield
2010-2017
Wellcome Trust
2016
Middlesex Hospital
1975
London School of Hygiene & Tropical Medicine
1975
Osteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning progression are incompletely understood. We investigated genes pathways that mark OA in isolated primary chondrocytes taken from paired intact versus degraded articular samples across 38 patients undergoing replacement surgery (discovery cohort: 12 knee OA, replication cohorts: 17 9 hip patients). combined genome-wide DNA methylation, RNA...
Structure-activity relationship studies of 2,8-disubstituted-1,5-naphthyridines, previously reported as potent inhibitors
1 step of PBS wash followed by quenching with 60% methanol supplemented 70 mM HEPES results in minimal metabolite leakage.
Vaginal secretions from 508 women were examined for evidence of infection by Trichomonas vaginalis, and antibodies directed against this organism; 42 (8-3 per cent.) found to be infected. Secretions 29 these assayed antibody apparently T. vaginalis was in 22 (76 them. Eight out nineteen (42 uninfected also contained antibody. Amongst the infected women, no correlation could between presence or absence degree inflammation, duration symptoms, use oral contraceptives, additional...
Plasmodium vivax is responsible for the majority of malaria cases outside Africa. Unlike P . falciparum , life-cycle includes a dormant liver stage, hypnozoite, which can cause infection in absence mosquito transmission. An effective vaccine against blood stages would limit symptoms and pathology from such recurrent infections, therefore could play critical role control this species. Vaccine development however, lags considerably behind has many identified targets with several having...
Plasmodium vivax causes the majority of malaria outside Africa, but is poorly understood at a cellular level partly due to technical difficulties in maintaining it vitro culture conditions. In past decades, drug resistant P. parasites have emerged, mainly Southeast Asia, while some molecular markers resistance been identified, none so far confirmed experimentally, which limits interpretation markers, and hence our ability monitor control spread resistance. Some these potential identified...
Abstract The recurrent emergence of drug resistance in Plasmodium falciparum increases the urgency to genetically validate mechanisms and identify new targets. Reverse genetics have facilitated genome-scale knockout screens berghei Toxoplasma gondii , which pooled transfections multiple vectors were critical increasing scale throughput. These approaches not yet been implemented human malaria species such as P. knowlesi part because extent can be performed these remains evaluated. Here we use...
The development of parasite resistance to both artemisinin derivatives and their partner drugs jeopardizes the effectiveness combination therapy. Thus, discovery new antimalarial drugs, with mechanisms action, is urgently needed. We recently disclosed that β-carboline 1a was orally efficacious in Plasmodium berghei-infected mice it showed low cross-resistance between susceptible falciparum four different drug-resistant strains. In this report, we describe synthesis vitro evaluation 91 1a....
Summary Several cohort studies have investigated the molecular basis of von Willebrand disease (VWD); however, these mostly focused on European and North American populations. This study aimed to investigate mutation spectrum in 26 index cases (IC) from Turkey diagnosed with all three VWD types, majority (73%) parents who were knowingly related. IC screened for mutations using multiplex ligation-dependent probe amplification analysis factor gene (VWF) exons exon/intron boundaries. Selected...
ABSTRACT Background Osteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning progression are incompletely understood, but can be using recent advances in genomics technologies, as the relevant tissue readily accessible at replacement surgery. Here we investigate genes pathways that mark OA progression, combining genome-wide DNA methylation, RNA sequencing quantitative proteomics isolated primary...
Summary Large-scale chemical-genetic screening, or chemogenomics, can faciliate rapid and scalable drug target identification. To establish a chemogenomic screen for antimalarial identification, we leveraged ∼600 Plasmodium berghei artificial chromosomes (PbACs) encoding potential targets to generate systematic overexpression library. PbACs were engineered with DNA barcodes, enabling their quantification within mixed pools using next generation sequencing (barcode BarSeq). Pooled...
<title>Abstract</title> Idiopathic Parkinson’s disease (iPD) is the second most common neurodegenerative after Alzheimer’s (AD). Mutations in SCNA gene, which encodes protein alpha synuclein (α-syn), are associated with familial forms of Parkinson's (PD). Additionally, Lewy bodies (LBs) rich α-synuclein a hallmark idiopathic pathology. Unlike AD, there no effective blood-based diagnostic assays for iPD. Recent studies show that measures misfolded α-syn cerebrospinal fluid (CSF) and skin...
Artemisinin-based combination therapy (ACT) is the mainstay of effective treatment Plasmodium falciparum malaria. However, long-term utility ACTs imperiled by widespread partial artemisinin resistance in Southeast Asia and its recent emergence parts East Africa. This underscores need to identify chemotypes with new modes action (MoAs) circumvent ACTs. In this study, we characterized asexual blood stage antiplasmodial activity mechanisms LDT-623, a 4-aminoquinoline (4-AQ). We also detected...
This immunofluorescence assay protocol is based on the work of Tonkin et al. (2004). We updated their method and used a 96-well plate format allowing medium-throughput method.
This is a recipe for media culturing P. knowlesi in vitro. The essentially identical to that used Moon et al. (2013), with the exception we tend use horse serum rather than human serum. resulting similar routine falciparum culture, but contains both and albumax components.
This protocol is based on that described in Moon et al. (2013): https://www.pnas.org/content/110/2/531
This protocol was developed to study protein expression in Plasmodium red blood stage. Large multispanning membrane proteins, such as pvmdr1 (PVX_080100), could be extracted and detected using this method.
ABSTRACT Plasmodium vivax is responsible for the majority of malaria cases outside Africa. Unlike P. falciparum , life-cycle includes a dormant liver stage, hypnozoite, which can cause infection in absence mosquito transmission. An effective vaccine against blood stages would limit symptoms and pathology from such recurrent infections, therefore could play critical role control this species. Vaccine development however, lags considerably behind has many identified targets with several having...