A5005367389- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Parasites and Host Interactions
- Trypanosoma species research and implications
- HIV/AIDS drug development and treatment
- Drug Transport and Resistance Mechanisms
- Research on Leishmaniasis Studies
- Drug-Induced Hepatotoxicity and Protection
- vaccines and immunoinformatics approaches
- Synthesis and biological activity
- Traditional and Medicinal Uses of Annonaceae
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Tuberculosis Research and Epidemiology
- Vector-borne infectious diseases
- Cancer therapeutics and mechanisms
- Phytochemistry and Bioactivity Studies
- Insect Pest Control Strategies
- Bird parasitology and diseases
- Genetics, Bioinformatics, and Biomedical Research
- Crystallization and Solubility Studies
- Helminth infection and control
- Enzyme function and inhibition
- X-ray Diffraction in Crystallography
Universidade Nova de Lisboa
2015-2024
University of Lisbon
2022
Universidade Evangelica de Goiás
2013-2021
Instituto de Medicina Tropical
2007-2019
Centro de Estudos Educação e Sociedade
2018
Secretaria do Meio Ambiente
2018
Universidade Federal de Goiás
2011-2017
Fundação de Apoio a Pesquisa do Estado de Goiás
2015
Infraestruturas de Portugal (Portugal)
1999-2012
Instituto de Olhos de Goiânia
2011
Malaria was eliminated from southern and southeastern Brazil over 50 years ago. However, an increasing number of autochthonous episodes attributed to Plasmodium vivax have recently been reported the Atlantic Forest region Rio de Janeiro state. As P vivax-like non-human primate malaria parasite species simium is locally enzootic, we performed a molecular epidemiological investigation determine whether zoonotic transmission occurring.We examined blood samples patients presenting with signs or...
Abstract Background Evaluating copy numbers of given genes in Plasmodium falciparum parasites is major importance for laboratory-based studies or epidemiological surveys. For instance, pfmdr1 gene amplification has been associated with resistance to quinine derivatives and several involved anti-oxidant defence may play an important role antimalarial drugs, although their potential involvement overlooked. Methods The ΔΔ Ct method relative quantification using real-time quantitative PCR SYBR...
Resistance of Plasmodium falciparum to drugs such as chloroquine and sulfadoxine-pyrimethamine is a major problem in malaria control. Artemisinin (ART) derivatives, particularly combination with other drugs, are thus increasingly used treat malaria, reducing the probability that parasites resistant components will emerge. Although stable resistance artemisinin has yet be reported from laboratory or field studies, its emergence would disastrous because lack alternative treatments. Here, we...
Summary Artemisinin‐ and artesunate‐resistant Plasmodium chabaudi mutants, AS‐ART AS‐ATN, were previously selected from chloroquine‐resistant clones AS‐30CQ AS‐15CQ respectively. Now, a genetic cross between the artemisinin‐sensitive clone AJ has been analysed by Linkage Group Selection. A linkage group on chromosome 2 was under artemisinin treatment. Within this locus, we identified two different mutations in gene encoding deubiquitinating enzyme. distinct mutation occurred each of relative...
Schistosomiasis is a neglected parasitic tropical disease that claims around 200,000 human lives every year. Praziquantel (PZQ), the only drug recommended by World Health Organization for treatment and control of schistosomiasis, now facing threat resistance, indicating urgent need new effective compounds to treat this disease. Therefore, globally, there renewed interest in natural products (NPs) as starting point discovery development schistosomiasis. Recent advances genomics, proteomics,...
The global emergence of Plasmodium vivax strains resistant to chloroquine (CQ) since the late 1980s is complicating current international efforts for malaria control and elimination. Furthermore, CQ-resistant has already reached an alarming prevalence in Indonesia, East Timor Papua New Guinea. More recently, vivo studies have documented P. infections Guyana, Peru Brazil. Here, we summarise available data on CQ resistance across vivax-endemic areas Latin America by combining published vitro...
Plasmodium vivax is estimated to affect 75 million people annually. It reportedly absent, however, from west and central Africa due the high prevalence of Duffy negative phenotype in indigenous populations. Despite this, non-African travellers consistently return their own countries with P. malaria after visiting this region. An attempt was made, therefore, detect presence parasites blood samples collected populations Africa. Parasite species typing (for all four human parasites) carried out...
Classical and quantitative linkage analyses of genetic crosses have traditionally been used to map genes interest, such as those conferring chloroquine or quinine resistance in malaria parasites. Next-generation sequencing technologies now present the possibility determining genome-wide variation at single base-pair resolution. Here, we combine vivo experimental evolution, a rapid strategy whole genome re-sequencing identify precise basis artemisinin lineage rodent parasite, Plasmodium...
The control of malaria, caused by Plasmodium falciparum, is hampered the relentless evolution drug resistance. Because artemisinin derivatives are now used in most effective anti-malarial therapy, resistance to would be catastrophic. Indeed, studies suggest that has already appeared natural infections. Understanding mechanisms help prolong lifetime these drugs. Genetic markers therefore required urgently. Previously, a mutation de-ubiquitinating enzyme was shown confer rodent malaria...
Morbidity and mortality caused by schistosomiasis are serious public health problems in developing countries. Because praziquantel is the only drug therapeutic use, risk of resistance a concern. In search for new schistosomicidal drugs, we performed target-based chemogenomics screen dataset 2,114 proteins to identify drugs that approved clinical use humans may be active against multiple life stages Schistosoma mansoni. Each these was treated as potential target, its amino acid sequence used...
Plasmodium vivax, the second most prevalent of human malaria parasites, is estimated to affect 75 million people annually. It very rare, however, in west and central Africa, due high prevalence Duffy negative phenotype population. Due its rarity previous studies on phylogeny world-wide P. vivax have suffered from insufficient samples African parasites. Here we compare mitochondrial sequence diversity parasites Africa with those other areas world, order investigate origin present-day vivax....
The dichloromethane, methanol and aqueous ethanol extracts of the stem bark Pycnanthus angolensis were evaluated for their in vitro activity against 3D7 Plasmodium falciparum strain. CH (2)Cl (2) extract was most active showing an IC (50) = 1.6 microg/mL. From this extract, a new dibenzylbutane lignan, threo-4,4'-dihydroxy-3-methoxylignan ( 1) named pycnantolol, together with known lignans (-)-dihydroguaiaretic acid 2), heliobuphthalmin 3), talaumidin 4), hinokinin 5), labdane-type diterpene...
Artemisinin, a thapsigargin-like sesquiterpene has been shown to inhibit the Plasmodium falciparum sarco/endoplasmic reticulum calcium-ATPase PfSERCA. To collect baseline pfserca sequence information before field deployment of Artemisinin-based Combination therapies that may select mutant parasites, we conducted analysis 100 isolates from multiple sites in Africa, Asia and South America. Coding diversity was large, with 29 mutated codons, including 32 SNPs (average one SNP/115 bp), which 19...
ABSTRACT Multidrug-resistant Plasmodium falciparum malaria parasites pose a threat to effective drug control, even artemisinin-based combination therapies (ACTs). Here we used linkage group selection and Solexa whole-genome resequencing investigate the genetic basis of resistance component drugs ACTs. Using rodent parasite P. chabaudi , analyzed uncloned progeny backcross between mefloquine-, lumefantrine-, artemisinin-resistant mutant AS-15MF genetically distinct sensitive clone, AJ,...
Abstract Background Drug resistance in the malaria parasite Plasmodium falciparum severely compromises treatment and control of malaria. A knowledge critical mutations conferring to particular drugs is important understanding modes drug action mechanisms resistances. They are required design better therapies limit resistance. mutation gene ( pfcrt ) encoding a membrane transporter has been identified as principal determinant chloroquine P . , but we lack full account higher level...
The increasing levels of Plasmodium falciparum resistance to chloroquine (CQ) in Thailand have led the use alternative antimalarials, which are at present also becoming ineffective. In this context, any strategies that help improve surveillance drug resistance, become crucial overcoming problem. study, we established vitro sensitivity CQ, mefloquine (MF), quinine (QUIN) and amodiaquine (AMQ) 52 P. isolates collected Thailand, assessed prevalence four putative genetic polymorphisms pfcrt...
The genetic determinants of resistance to mefloquine in malaria parasites are unclear. Some studies have implied that amplification of, or mutations in, the multidrug gene pfmdr1 Plasmodium falciparum may be involved. Using rodent model chabaudi, we investigated role orthologue this gene, pcmdr1, a stable mefloquine-resistant mutant, AS(15MF/3), selected from sensitive clone. pcmdr1 exists as single copy on chromosome 12 In was found undergone duplication, with one translocating 4. mRNA...
Summary Objective To evaluate the in vitro efficacy of artesunate (ATN) and artemether (ATH) against Plasmodium falciparum isolates from Brazilian Amazon state Pará to search for mutations and/or altered copy numbers putative resistance‐associated pfcrt , pfmdr1 pfATPase6 genes. Methods In ATN ATH was successfully measured 56 freshly collected P. isolates, using a conventional WHO microtest with minor modifications. Single nucleotide polymorphisms (SNPs) same were inspected DNA sequencing...