A5039135020- Neurobiology and Insect Physiology Research
- Parasite Biology and Host Interactions
- RNA and protein synthesis mechanisms
- Animal Behavior and Reproduction
- Insect Resistance and Genetics
- RNA modifications and cancer
- Parasites and Host Interactions
- Insect and Arachnid Ecology and Behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Phylogenetic Studies
- Extracellular vesicles in disease
- Immune cells in cancer
- Developmental Biology and Gene Regulation
- Nanoplatforms for cancer theranostics
- Insect symbiosis and bacterial influences
- Hemiptera Insect Studies
- Immune Cell Function and Interaction
- Phytoplasmas and Hemiptera pathogens
- Video Surveillance and Tracking Methods
- PARP inhibition in cancer therapy
- Genomics and Chromatin Dynamics
- BRCA gene mutations in cancer
- Connective tissue disorders research
- Advanced biosensing and bioanalysis techniques
- Orthoptera Research and Taxonomy
Harvard University
2017-2025
Brigham and Women's Hospital
2021-2024
Center for Systems Biology
2024
Institut de Biologia Evolutiva
2020
Max Planck Institute for Developmental Biology
2013-2016
University of Southern California
2011
Taisei Kikuchi, Mark Viney, Matthew Berriman and colleagues report the genome sequences of six species nematodes from Strongyloides clade nematodes, including human animal pathogens, facultative parasites a free-living species. They find that expansions astacin SCP/TAPS gene families are associated with parasitism in these Soil-transmitted genus, cause one most prevalent neglected tropical diseases. Here we compare genomes four species, pathogen stercoralis, their close relatives...
Herpes simplex viruses (HSVs) are prevalent human pathogens that establish latency in neuronal cells and efficiently evade the immune system. It has been a major medical challenge to eradicate them and, despite intensive efforts, an effective vaccine is not available. We previously showed upon infection of antigen-presenting cells, HSV type 1 (HSV-1) rapidly downregulates histocompatibility complex class I-like molecule, CD1d, potently inhibits its recognition by CD1d-restricted natural...
Cancer cells program fibroblasts into cancer associated (CAFs) in a two-step manner. First, secrete exosomes to quiescent activated CAFs. Second, maintain the CAF phenotype via activation of signal transduction pathways. We rationalized that inhibiting this process can normalize CAFs and augment efficacy immunotherapy. show cell–targeted nanoliposomes inhibit sequential steps exosome biogenesis release from lung block differentiation In parallel, we demonstrate CAF-targeted two distinct...
Single-cell genomics encompasses a set of methods whereby hundreds to millions cells are individually subjected multiplexed assays including sequencing DNA, chromatin accessibility or modification, RNA, combinations thereof 1,2. These enable unbiased, systematic discovery cellular phenotypes and their dynamics 1-3. Many functional genomic methods, however, require multiple steps that cannot be easily scaled high throughput, on living cells. Here we develop capsules with amphiphilic gel...
The faster-X effect, namely the rapid evolution of protein-coding genes on X chromosome, has been widely reported in metazoans. However, prevalence this phenomenon across diverse systems and its potential causes remain largely unresolved. Analysis sex-biased may elucidate possible mechanisms: for example, with X/Y males a more pronounced effect male-biased than female-biased or unbiased suggest fixation recessive beneficial mutations rather genetic drift. Further, theory predicts that should...
Sex-biased gene expression, particularly sex-biased expression in the gonad, has been linked to rates of protein sequence evolution (nonsynonymous synonymous substitutions, dN/dS) animals. However, insects, studies remain centred on a few holometabolous species. Moreover, other major tissue types such as brain underexplored. Here, we studied and hemimetabolous insect, cricket Gryllus bimaculatus. We generated novel male female RNA-seq data for two sexual types, gonad somatic reproductive...
Novel genes have the potential to drive evolution of new biological mechanisms, or integrate into preexisting regulatory circuits and contribute regulation older, conserved functions. One such gene, novel insect-specific gene
SUMMARY The parasitic roundworms Strongyloides stercoralis (in man) and ratti rats) employ environmentally controlled XX/XO sex determination with a pair of X chromosomes two pairs autosomes. papillosus sheep) has only chromosomes, one which combines the genetic material homologous to S. I. This species creates males through elimination copy portion related chromosome (chromatin diminution). It is not clear these sex-determining mechanisms ancestral. We demonstrate that vituli cattle) like...
Abstract Background For multicellular organisms, much remains unknown about the dynamics of synonymous codon and amino acid use in highly expressed genes, including whether their varies with expression different tissue types sexes. Moreover, specific codons acids may have translational functions transcribed that largely depend on relationships to tRNA gene copies genome. However, these putative are poorly understood, particularly systems. Results Here, we studied genes from reproductive...
Abstract Sex-biased gene expression, particularly sex-biased expression in the gonad, has been linked to rates of protein sequence evolution (nonsynonymous synonymous substitutions, dN/dS) animals. However, insects, studies remain centered on a few holometabolous species, and moreover, other major tissue types such as brain underexplored. Here, we studied hemimetabolous insect, cricket Gryllus bimaculatus . We generated novel male female RNA-seq data for two sexual types, gonad somatic...
Allogeneic natural killer (aNK) cell adoptive therapy has the potential to dramatically impact clinical outcomes of glioblastoma multiforme (GBM). However, in order exert therapeutic activity, NK cells require tumor expression ligands for activating receptors, such as MHC Class I peptide A/B (MICA/B) and ULBPs. Here, we describe use a blood-brain barrier (BBB) permissive supramolecular cationic drug vehicle comprising an inhibitor chaperone heat shock protein 90 (Hsp90), which sustains...
Nematodes of the genus Strongyloides are important parasites vertebrates including man. Currently, little is known about their germline organization or reproductive biology and how this influences parasitic life strategies. Here, we analyze structure in several closely related species uncover striking differences development, organization, fluid dynamics compared to model organism Caenorhabditis elegans. With a focus on ratti, reveal that proliferation germ cells restricted early mid-larval...
Abstract Synonymous codon use is non-random. Codons most used in highly transcribed genes, often called optimal codons, typically have high gene counts of matching tRNA genes (tRNA abundance) and promote accurate and/or efficient translation. Non-optimal those least expressed may also affect In multicellular organisms, optimality vary among tissues. At present, however, tissue specificity remains poorly understood. Here, we studied usage germ line (testis ovary) somatic tissues...
Evolutionary developmental biology focuses on understanding the origin and evolution of extant biological variation, genetic basis for this variation. The toolkit appears largely finite across animals, such that a combination regulatory evolution, gene recruitment (co-option) modularity often allow morphological diversity to arise. Here we summarize number observations from which together suggest many genes product interaction "modules" originally characterized their role in germ line also...
Abstract Background The faster-X effect, namely the rapid evolution of protein-coding genes on X-chromosome, has been reported in numerous metazoans. However, prevalence this phenomenon across metazoans and its potential causes remain largely unresolved. Analysis sex-biased may elucidate possible mechanisms: a more pronounced effect male-biased than female-biased or unbiased genes, suggests fixation recessive beneficial mutations rather genetic drift. Further, theory predicts that should be...
Abstract Synonymous codon use is non-random. Codons most used in highly transcribed genes, often called optimal codons, typically have high gene counts of matching tRNA genes (tRNA abundance) and promote accurate and/or efficient translation. Non-optimal those least expressed may also affect In multicellular organisms, optimality vary among tissues. At present however, remains poorly understood organisms. Here, we studied usage germ line (testis, ovary) somatic tissues (gonadectomized males...
Abstract Background For multicellular organisms, much remains unknown about the dynamics of synonymous codon and amino acid use in highly expressed genes, including whether their varies with expression different tissue types sexes. Moreover, specific codons acids may have translational functions transcribed that largely depend on relationships to tRNA gene copies genome. However, these putative are poorly understood, particularly systems. Results Here, we rigorously studied genes from...
In this paper, we proposed an idea to estimate the speed of vehicle and track over speeding vehicle. We use two sensors one at initial point other tracking point. When sensor senses moving it captures image a time recorder will record time. compare frames captured points if match If estimated is less than initially fixed time-limit then considered be speeded that