A5102712770- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Insect and Pesticide Research
- Insect Utilization and Effects
- Aquaculture Nutrition and Growth
- Metabolism and Genetic Disorders
- Physiological and biochemical adaptations
- Receptor Mechanisms and Signaling
- Biotin and Related Studies
- Microbial infections and disease research
- Immunodeficiency and Autoimmune Disorders
- Food Quality and Safety Studies
- Thyroid Disorders and Treatments
- Tactile and Sensory Interactions
- Memory and Neural Mechanisms
- Diabetes Treatment and Management
- Reproductive biology and impacts on aquatic species
- Neuroscience and Neuropharmacology Research
- Immune Cell Function and Interaction
- Gastric Cancer Management and Outcomes
- Pharmacy and Medical Practices
- Oral and gingival health research
- Herpesvirus Infections and Treatments
- Meat and Animal Product Quality
The Graduate University for Advanced Studies, SOKENDAI
2024-2025
Shizuoka University
2023
Hokkaido University
2013-2023
National Society of Professional Engineers
2022
Akita Research Institute for Food and Brewing
2006-2022
Shibuya (Japan)
2022
Nagaoka Red Cross Hospital
2022
Nihon Pharmaceutical University
2021
Nihon University
2021
Yamaha (Japan)
2013
OBJECTIVE This 24-week, double-blinded, phase 3 clinical trial (DEPICT-2; ClinicalTrials.gov, NCT02460978) evaluated efficacy and safety of dapagliflozin as adjunct therapy to adjustable insulin in patients with inadequately controlled type 1 diabetes (HbA1c 7.5–10.5%). RESEARCH DESIGN AND METHODS Patients were randomized 1:1:1 5 mg (n = 271), 10 270), or placebo 272) plus insulin. Insulin dose was adjusted by investigators according self-monitored glucose readings, local guidance,...
Using an oxygen radical absorbance capacity (ORAC) assay, antioxidant activity was detected in the ethanol extract of Pacific oyster, which purified by sequential extraction with organic solvents. The ethyl acetate fraction showed strongest and further purified, yielding a single compound [as assessed thin-layer chromatography (TLC) reverse-phase high-performance liquid (HPLC)]. This identified as 3,5-dihydroxy-4-methoxybenzyl alcohol on basis 1H 13C nuclear magnetic resonance (NMR),...
AbstractClostridium botulinum strains produce seven immunologically distinct neurotoxins (NTX), type A to G. the NTXs associate with nontoxic components in cultures, and become large complexes three forms (12S, 16S, 19S) designated progenitor toxins. 12S toxin consists of a NTX component having no hemagglutinin (HA) activity (described here as non-toxic non-HA, NTNH), 16S 19S toxins are formed by conjugation HA. Based on genetic-and protein chemical-analyses it became clear that 1) HA four...
Entrainment to the environmental light cycle is an essential property of circadian clock. Although compound eye known be major photoreceptor necessary for entrainment in many insects, molecular mechanisms photic remain explored.We found that cryptochromes (crys) and c-fos mediate clock a hemimetabolous insect, cricket Gryllus bimaculatus. We examined effects RNA interference (RNAi)-mediated knockdown cry genes, Gb'cry1 Gb'cry2, on entrainment, light-induced resetting locomotor rhythm....
Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is heteromeric complex composed ligand-specific receptor and the co-receptor (ORco). In this study, we identified ORco gene cockroach, Periplaneta americana (PameORco), performed RNAi-based functional analysis PameORco. All OSNs basiconic sensilla PameORco received including sex pheromones. trichoid sensilla, PameORco-positive OSN was consistently paired with...
In the majority of studies designed to elucidate causal mechanisms memory formation, certain members experimental cohort, even though subjected exactly same conditioning procedures, remember significantly better than others, whereas others show little or no long-term (LTM)formation. To begin address question why this phenomenon occurs and thereby help clarify mechanism LTM we used a conditioned taste aversion (CTA) procedure on individuals pond snail Lymnaea stagnalis analyzed their...
SUMMARY Conditioned taste aversion (CTA) in the pond snail Lymnaea stagnalis has been widely used as a model for gaining an understanding of molecular and behavioral mechanisms underlying learning memory. At level, however, it is still unclear how discrimination CTA interact. We thus examined to one affected feeding response induced by another appetitive food stimulus. first demonstrated that snails have capacity recognize sucrose carrot juice distinct stimuli. then found can become...
The present study was conducted by screening zein-degrading bacteria in an attempt to obtain protease.Soil were screened formation of a clear zone on zein plates. Characterization bacterium indicated taxonomic affiliation Bacillus pumilus, and named MS-1 strain. strain produced two different types extracellular proteases, BPP-A BPP-B. In this study, we purified characterized because it exhibited higher ability hydrolyze than When casein used as the substrate, optimal pH for 11.0. BPP-A,...
The ecdysone receptor (EcR) regulates various cellular responses to ecdysteroids during insect development. Insects have multiple EcR isoforms with different N-terminal A/B domains that contain the isoform-specific activation function (AF)-1 region. Although distinct physiologic functions of been characterized in higher holometabolous insects, they remain unclear basal direct-developing which only A isoform has identified. To examine structural basis AF-1 regions, we performed a...
Abstract Serotonin (5‐HT) modulates various aspects of behaviours such as aggressive behaviour and circadian in the cricket. To elucidate molecular basis cricket 5‐HT system, we identified 5‐HT‐related genes field Gryllus bimaculatus DeGeer. Complementary DNA tryptophan hydroxylase phenylalanine‐tryptophan hydroxylase, which convert into 5‐hydroxy‐L‐tryptophan (5‐HTP), that aromatic L‐amino acid decarboxylase, converts 5‐HTP 5‐HT, were isolated from a brain cDNA library. In addition, four...
In Lymnaea, experiments showed that one-day food deprivation before aversive classical conditioning results in optimal conditioned taste aversion (CTA) and long-term memory (LTM), whereas 5-day training did not. We hypothesized snails do fact learn form LTM when trained after prolonged deprivation, but severe blocks their ability to express memory. food-deprived under various conditions, found was indeed formed overwhelmed by deprivation. Moreover, CTA-LTM context-dependent could be observed...
Abstract Many animals use multicomponent sex pheromones for mating, but the specific function and neural processing of each pheromone component remain unclear. The cockroach Periplaneta americana is a model studying communication, an adult female emits major minor components, periplanone-B -A (PB PA), respectively. Attraction courtship behaviors (wing-raising abdominal extension) are strongly expressed when males exposed to PB weakly they PA. When presented together with PA, elicited by were...
We examined changes in the content of biogenic amines brains ant queen associated with early colony establishment. In ants, including Formica japonica, winged virgin queens lose their wings following copulation, and then start establishing a colony. Significant brain amine are transition from to wingless mated queen. The levels serotonin (5HT), octopamine (OA) dopamine (DA) decreased significantly after starting On other hand, tyramine (TA) increased Catabolized substances were also...
We investigated in this study the anti-obesity effect of an extract Petasites japonicus (a culinary vegetable from Eastern Asia) on a murine adipocyte cell line (3T3-L1) and diet-induced obesity-prone mice. An ethanol P. japonicus. (PJET) suppressed 3T3-L1 preadipocyte differentiation; however, hot water (PJHW) exhibited no differentiation. PJET significantly attenuated three adipogenetic transcription factors, peroxisome proliferator-activated receptor gamma2, CCAAT/enhancer-binding protein...
ABSTRACT Cannabinoids are hypothesized to play an important role in modulating learning and memory formation. Here, we identified mRNAs expressed Lymnaea stagnalis central nervous system that encode two G-protein-coupled receptors (Lymnaea CBr-like 1 2) structurally resemble mammalian cannabinoid (CBrs). We found injection of a CBr agonist WIN 55,212-2 (WIN 55) into the snail before operant conditioning obstructed This effect 55 persisted for at least 4 days following its injection. A...
The pond snail Lymnaea learns conditioned taste aversion (CTA) and remembers not to respond food substances that initially cause a feeding response. possible relationship between how well snails learn follow taste-aversion training brain dopamine contents is known. We examined this found the following: first, in act of eating just before commencement CTA were poor learners had highest brain; second, which an ad libitum access food, but training, average lower contents; third, food-deprived...