A5022397717- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Cassava research and cyanide
- Banana Cultivation and Research
- Remote Sensing in Agriculture
- Smart Agriculture and AI
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Leaf Properties and Growth Measurement
- Spectroscopy and Chemometric Analyses
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Soil Geostatistics and Mapping
- Cell Image Analysis Techniques
- Agricultural Development and Management
- Species Distribution and Climate Change
- Legume Nitrogen Fixing Symbiosis
- Agronomic Practices and Intercropping Systems
- Genetic and Environmental Crop Studies
- Plant Molecular Biology Research
- Genetic and phenotypic traits in livestock
- Mycorrhizal Fungi and Plant Interactions
- Nematode management and characterization studies
- Agroforestry and silvopastoral systems
Donald Danforth Plant Science Center
2016-2025
Bayer (United States)
2022-2025
Bayer (France)
2022
Washington University in St. Louis
2018-2021
The University of Texas at San Antonio
2013
Baylor School
2013
Baylor College of Medicine
2013
Tufts Medical Center
2002
Systems for collecting image data in conjunction with computer vision techniques are a powerful tool increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that flexible and extendable needed to address diversity of phenotyping problems. We previously described Plant Computer Vision (PlantCV) software package, is an processing toolkit analysis. The goal PlantCV project develop set modular, reusable, repurposable analysis...
Abstract Pathogens rely on expression of host susceptibility ( S ) genes to promote infection and disease. As DNA methylation is an epigenetic modification that affects gene expression, blocking access through targeted could increase disease resistance. Xanthomonas phaseoli pv. manihotis , the causal agent cassava bacterial blight (CBB), uses transcription activator-like20 (TAL20) induce MeSWEET10a . In this work, we direct TAL20 effector binding element within promoter using a synthetic...
Cassava (Manihot esculenta) feeds c. 800 million people world-wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA-sequencing develop an open access, web-based interface further interrogation of data. Through dataset, consider physiology cassava. Specifically, focus on...
Abstract Drought is a major abiotic stress limiting agricultural productivity. Previous field-level experiments have demonstrated that drought decreases microbiome diversity in the root and rhizosphere. How these changes ultimately affect plant health remains elusive. Toward this end, we combined reductionist, transitional ecological approaches, applied to staple cereal crop sorghum identify key root-associated microbes robustly drought-stressed phenotypes. Fifty-three Arabidopsis-associated...
Abstract C 4 plants frequently experience high light and temperature conditions in the field, which reduce growth yield. However, mechanisms underlying these stress responses have been under-explored, especially coordination between mesophyll (M) bundle sheath (BS) cells. We investigated how model plant Setaria viridis responded to a four-hour or treatment at photosynthetic, transcriptomic, ultrastructural levels. Although we observed comparable reduction of photosynthetic efficiency treated...
Abstract Different intensities of high temperatures affect the growth photosynthetic cells in nature. To elucidate underlying mechanisms, we cultivated unicellular green alga Chlamydomonas reinhardtii under highly controlled photobioreactor conditions and revealed systems-wide shared unique responses to 24-hour moderate (35°C) acute (40°C) subsequent recovery at 25°C. We identified previously overlooked elements response temperature. Heat 35°C transiently arrested cell cycle followed by...
The kidney glomerular capillaries are frequent sites of immune complex deposition and subsequent neutrophil accumulation in post-infectious rapidly progressive glomerulonephritis. However, the mechanisms recruitment remain enigmatic, there is no targeted therapeutic to avert this proximal event inflammation. uniquely human activating Fc receptor FcγRIIA promotes damage anti-glomerular basement membrane-induced (anti-GBM-induced) glomerulonephritis when expressed on murine neutrophils. Here,...
Visualization of cell migration via time-lapse microscopy has greatly advanced our understanding the immune system. However, subtle differences in dynamics are easily obscured by biases and imaging artifacts. While several analysis methods have been suggested to address these issues, an integrated tool implementing them is currently lacking. Here, we present celltrackR, R package containing a diverse set state-of-the-art for (immune) tracks. CelltrackR supports complete pipeline track...
High-throughput phenotyping has emerged as a powerful method for studying plant biology. Large image-based datasets are generated and analyzed with automated image analysis pipelines. A major challenge associated these analyses is variation in quality that can inadvertently bias results. Images made up of tuples data called pixels, which consist R, G, B values, arranged grid. Many factors, example brightness, influence the captured. These factors alter values pixels within images...
Cotton bacterial blight (CBB), an important disease of (Gossypium hirsutum) in the early 20th century, had been controlled by resistant germplasm for over half a century. Recently, CBB re-emerged as agronomic problem United States. Here, we report analysis cotton variety planting statistics that indicate steady increase percentage susceptible varieties grown each year since 2009. Phylogenetic revealed strains from current outbreak cluster with race 18 Xanthomonas citri pv. malvacearum (Xcm)...
Plant disease symptoms exhibit complex spatial and temporal patterns that are challenging to quantify. Image-based phenotyping approaches enable multidimensional characterization of host-microbe interactions well suited capture data key understanding progression. We applied image-based methods investigate cassava bacterial blight, which is caused by the pathogen Xanthomonas axonopodis pv. manihotis (Xam). generated Xam strains in individual predicted type III effector (T3E) genes were...
Cassava (Manihot esculenta Crantz, 2n = 36) is a global food security crop. It has highly heterozygous genome, high genetic load, and genotype-dependent asynchronous flowering. typically propagated by stem cuttings any variation between haplotypes, including large structural variations, preserved such clonal propagation. Traditional genome assembly approaches generate collapsed haplotype representation of the genome. In plants, this results in artifacts an oversimplification regions. We used...
With an increasing demand for renewable fuels, bioenergy crops are being developed with high sugar content and altered cell walls to improve processing efficiency. These traits may have unintended consequences plant disease resistance. Xanthomonas vasicola pv. holcicola (Xvh), the causal agent of sorghum bacterial leaf streak, is a wide-spread pathogen. Here, we show that Xvh expresses several wall degrading enzymes (CWDEs) during infection, these required full virulence. In tolerant...
CRISPR endonucleases require cognate non-coding RNA species for site-specific activity. These are typically expressed using endogenous polymerase III (Pol III) promoters compatible with the host species. This study describes applications of novel Pol promoters, which were computationally derived from a training set monocot U6 and U3 promoters. enabled genome editing in maize protoplast cells plants. Out 37 27 performed similarly to control promoter. Multiplexing five one construct...
Sorghum (Sorghum bicolor (L.) Moench) is a rapidly growing, high-biomass crop prized for abiotic stress tolerance. However, measuring genotype-by-environment (G x E) interactions remains progress bottleneck. We subjected panel of 30 genetically diverse sorghum genotypes to spectrum nitrogen deprivation and measured responses using high-throughput phenotyping technology followed by ionomic profiling. Responses were quantified shape (16 measurable outputs), color (hue intensity), ionome (18...
Methods to accurately quantify disease severity are fundamental plant pathogen interaction studies. Commonly used methods include visual scoring of symptoms, tracking growth in planta over time, and various assays that detect defense responses. Several image-based for phenotyping symptoms have also been developed. Each these has different advantages limitations which should be carefully considered when choosing an approach interpreting the results.In this paper, we developed two image...
Corticotropin releasing hormone (CRH) and interleukin-6 (IL-6) are implicated in inflammatory diseases triggered by stress. Acute restraint stress increases serum IL-6 the blood, but its source is not known. Our current study was carried out order to determine contribution of mast cells stress-induced release investigate skin CRH vascular permeability mice. W/W(v) cell deficient their wild type control +/+ mice were stressed a plexiglass chamber for 60 or 120 min. Serum corticosterone levels...
Abstract Plant disease symptoms exhibit complex spatial and temporal patterns that are challenging to quantify. Image-based phenotyping approaches enable multi-dimensional characterization of host-microbe interactions well suited capture data key understanding progression. We applied image-based methods investigate cassava bacterial blight, which is caused by the pathogen Xanthomonas axonopodis pv. manihotis ( Xam ). generated strains in individual predicted type III effector (T3E) genes...
Angiogenesis, new blood vessel formation from preexisting vessels, is critical for solid tumor growth. As such, there have been efforts to inhibit angiogenesis as a means obstruct However, antiangiogenic therapy faces major challenges the selective targeting of tumor-associated-vessels, current targets also disrupt steady-state vessels. Here, we demonstrate that developmentally transcription factor Etv2 selectively upregulated in both human and mouse tumor-associated endothelial cells...
RNA-based silencing functions as an important antiviral immunity mechanism in plants. Plant viruses evolved to encode viral suppressors of RNA (VSRs) that interfere with the function key components pathway. As effectors pathway, ARGONAUTE (AGO) proteins are targeted by some VSRs, such encoded Turnip crinkle virus (TCV). A VSR-deficient TCV mutant was used identify AGO activities during infection. quantitative phenotyping protocol using image-based color trait analysis pipeline on PlantCV...
Quinoa is a popular seed crop, often consumed for its high nutritional quality. We studied how heat stress in the roots or shoots of quinoa plants affected concentrations 20 elements (aluminum, arsenic, boron, calcium, cadmium, cobalt, copper, iron, potassium, magnesium, manganese, molybdenum, sodium, nickel, phosphorous, rubidium, sulfur, selenium, strontium, and zinc) seed. Elemental were significantly changed after an 11-day treatment during anthesis. The type panicle (main, secondary,...
Abstract Summary Visualization of cell migration via time-lapse microscopy has greatly advanced our understanding the immune system. However, subtle differences in dynamics are easily obscured by biases and imaging artifacts. While several analysis methods have been suggested to address these issues, an integrated tool implementing them is currently lacking. Here, we present CelltrackR, R package containing a diverse set state-of-the-art for (immune) tracks. CelltrackR supports complete...
The zig-zag model of host-pathogen interaction describes the relative strength defense response across a spectrum pathogen-induced plant phenotypes. A stronger results in increased resistance. Here, we investigate pathogen virulence during disease and place these findings context model. Xanthomonas vasicola pv. holcicola ( Xvh ) causes sorghum bacterial leaf streak. Despite being widespread, this has not been described detail at molecular level. We divided diverse genotypes into three groups...
Systems for collecting image data in conjunction with computer vision techniques are a powerful tool increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that flexible and extendable needed to address diversity of phenotyping problems. We previously described Plant Computer Vision (PlantCV) software package, is an processing toolkit analysis. The goal PlantCV project develop set modular, reusable, repurposable analysis...