A5050673789- Cassava research and cyanide
- Plant tissue culture and regeneration
- Computational Fluid Dynamics and Aerodynamics
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Nematode management and characterization studies
- Plant Micronutrient Interactions and Effects
- Fluid Dynamics and Turbulent Flows
- Banana Cultivation and Research
- Advanced Numerical Analysis Techniques
- Computational Geometry and Mesh Generation
- Gas Dynamics and Kinetic Theory
- Plant Pathogenic Bacteria Studies
- Aerodynamics and Acoustics in Jet Flows
- Transgenic Plants and Applications
- Wind and Air Flow Studies
- Seed Germination and Physiology
- Probabilistic and Robust Engineering Design
- Aerospace and Aviation Technology
- Plant responses to water stress
- Chromosomal and Genetic Variations
- Plant Physiology and Cultivation Studies
- Nuclear Engineering Thermal-Hydraulics
- Manufacturing Process and Optimization
- Computer Graphics and Visualization Techniques
Donald Danforth Plant Science Center
2016-2025
University of Exeter
2023-2025
MBDA (United Kingdom)
2015-2024
MBDA (Germany)
2014-2022
University of Glasgow
2017-2018
University of Pretoria
2015
University of Missouri–St. Louis
2012-2013
Saint Louis University
2004
Defence Science and Technology Agency
2004
Royal Botanic Gardens, Kew
2002
Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and Central Africa threatens production West Africa. CBSD caused by two species of positive-sense RNA viruses belonging to the family Potyviridae, genus Ipomovirus: virus (CBSV) Ugandan (UCBSV). Diseases Potyviridae require interaction viral genome-linked protein (VPg) host eukaryotic translation initiation factor 4E (eIF4E) isoforms. encodes five eIF4E proteins: eIF4E, eIF(iso)4E-1, eIF(iso)4E-2, novel...
CRISPR/Cas9 has become a powerful genome-editing tool for introducing genetic changes into crop species. In order to develop capacity technology in the tropical staple cassava (Manihot esculenta), Phytoene desaturase (MePDS) gene was targeted two cultivars using constructs carrying gRNAs targeting sequences within MePDS exon 13. After Agrobacterium-mediated delivery of reagents cells, both induced visible albino phenotypes cotyledon-stage somatic embryos regenerating on selection medium and...
Summary Effective weed control can protect yields of cassava ( Manihot esculenta ) storage roots. Farmers could benefit from using herbicide with a tolerant cultivar. We applied traditional transgenesis and gene editing to generate robust glyphosate tolerance in cassava. By comparing promoters regulating expression transformed 5‐enolpyruvylshikimate‐3‐phosphate synthase EPSPS genes various paired amino acid substitutions, we found that strong constitutive is required achieve during vitro...
DNA methylation is important for the regulation of gene expression and silencing transposons in plants. Here we present genome-wide patterns at single-base pair resolution cassava (Manihot esculenta, cultivar TME 7), a crop with substantial impact agriculture subtropical tropical regions. On average, levels were higher all three sequence contexts (CG, CHG, CHH, where H equals A, T, or C) than those most well-studied model plant Arabidopsis thaliana. As other plants, was found both on...
Cassava brown streak disease (CBSD) is emerging as one of the most important viral diseases cassava (Manihot esculenta) and considered today biggest threat to cultivation in East Africa. The caused by isolates at least two phylogenetically distinct species single-stranded RNA viruses belonging family Potyviridae, genus Ipomovirus. are present predominantly coastal lowland [Cassava virus (CBSV); Tanzania Mozambique] highland Uganda (CBSUV); Lake Victoria Basin, Uganda, Kenya Malawi] In this...
Less than 10% of the estimated average requirement (EAR) for iron and zinc is provided by consumption storage roots staple crop cassava (Manihot esculenta Crantz) in West African human populations. We used genetic engineering to improve mineral micronutrient concentrations cassava. Overexpression Arabidopsis thaliana vacuolar transporter VIT1 accumulated three- seven-times-higher levels transgenic nontransgenic controls confined field trials Puerto Rico. Plants engineered coexpress a mutated...
Cassava (Manihot esculenta) feeds c. 800 million people world-wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA-sequencing develop an open access, web-based interface further interrogation of data. Through dataset, consider physiology cassava. Specifically, focus on...
Nigeria is the world's largest producer of cassava (Manihot esculenta), and its production important to country's economy. Cassava's edible storage roots act as a critical staple food for over 180 million Nigerians. Micronutrient deficiency presents major public health issue in correlates with consumption level across six-agro-ecological zones within country. Though high caloric value, are deficient minerals, placing populations that rely on this crop at risk hidden hunger. deficiencies,...
Cassava (Manihot esculenta) is a starchy root crop that supports over billion people in tropical and subtropical regions of the world. This staple, however, produces neurotoxin cyanide requires processing for safe consumption. Excessive consumption insufficiently processed cassava, combination with protein-poor diets, can have neurodegenerative impacts. problem further exacerbated by drought conditions which increase this toxin plant. To reduce levels we used CRISPR-mediated mutagenesis to...
SUMMARY Plant transformation is an important part of plant research and crop improvement. Transformation methods remain complex, labor intensive, inefficient. PlantGENE a community scientists from academia, industry, non‐profit institutes, government organizations working to improve transformation. hosts virtual training, interactive webinars, website with career opportunities, directories, more. The science has shown great interest support for PlantGENE.
Cassava brown streak disease (CBSD), caused by Uganda virus (CBSUV) and (CBSV), is of new epidemic importance to cassava (Manihot esculenta Crantz) production in East Africa, an emerging threat the crop Central West Africa. This study demonstrates that at least one these two ipomoviruses, CBSUV, can be efficiently controlled using RNA interference (RNAi) technology cassava. An RNAi construct targeting near full-length coat protein (FL-CP) CBSUV was expressed constitutively as a hairpin...
Summary Storage roots of cassava ( Manihot esculenta Crantz), a major subsistence crop sub‐Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A β‐carotene. In this study, β‐carotene concentrations storage were enhanced by co‐expression transgenes for deoxy‐ d ‐xylulose‐5‐phosphate synthase DXS ) and bacterial phytoene crtB ), mediated the patatin‐type 1 promoter. harvested from field‐grown plants accumulated carotenoids to ≤50 μg/g DW , 15‐...
Cassava brown streak disease (CBSD) threatens food and economic security for smallholder farmers throughout East Central Africa, poses a threat to cassava production in West Africa. CBSD is caused by two whitefly-transmitted virus species: (CBSV) Ugandan (UCBSV) (Genus: Ipomovirus, Family Potyviridae). Although varying levels of tolerance have been achieved through conventional breeding, date, effective resistance within African germplasm has not identified. RNAi technology was utilized...
Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of two causal agents Cassava brown streak disease (CBSD): virus (CBSV) and Ugandan cassava (UCBSV). Transient expression studies on ten amiRNAs 21nt P1(CBSV UCBSV), P3(CBSV CI(UCBSV), NIb(CBSV CP(UCBSV) un-translated region (3'-UTR) tested in Nicotiana benthamiana. Four out expressed corresponding amiRNA at high levels. Transgenic N. benthamiana plants developed for four P1 NIb genes CBSV CP UCBSV...
Summary Tef is a staple food and valuable cash crop for millions of people in Ethiopia. Lodging major limitation to tef production, decades, the development lodging resistant varieties proved difficult with conventional breeding approaches. We used CRISPR/Cas9 introduce knockout mutations orthologue rice SEMIDWARF‐1 ( SD‐1 ) gene confer semidwarfism ultimately resistance. High frequency recovery transgenic edited lines was achieved two cultivars by Agrobacterium ‐mediated delivery into young...
Abstract Background Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting plants is required facilitate their molecular study and essential for development protection improvement programs. Retaining integrity pathogens within sampled plant tissues often a limiting factor in this process, most especially when sample sizes are large operating developing counties regions remote from laboratory facilities. FTA paper-based system designed...