A5100344775- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- RNA modifications and cancer
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- FOXO transcription factor regulation
- Gut microbiota and health
- Sulfur Compounds in Biology
- Breast Cancer Treatment Studies
- Plant Stress Responses and Tolerance
- Advanced battery technologies research
- Plant Gene Expression Analysis
- Photoreceptor and optogenetics research
Shenyang Aerospace University
2024
Yale University
2023-2024
Dalian Medical University
2015-2024
First Affiliated Hospital of Dalian Medical University
2015-2024
Tianjin Medical University Cancer Institute and Hospital
2023-2024
University of Jinan
2024
Capital Medical University
2024
Yale Cancer Center
2023-2024
Chongqing University
2023
Donald Danforth Plant Science Center
2020-2023
The capability to spatially explore RNA biology in formalin-fixed paraffin-embedded (FFPE) tissues holds transformative potential for histopathology research. Here, we present pathology-compatible deterministic barcoding tissue (Patho-DBiT) by combining situ polyadenylation and computational innovation spatial whole transcriptome sequencing, tailored probe the diverse species clinically archived FFPE samples. It permits co-profiling of gene expression processing, unveiling region-specific...
Abstract C 4 plants frequently experience high light and temperature conditions in the field, which reduce growth yield. However, mechanisms underlying these stress responses have been under-explored, especially coordination between mesophyll (M) bundle sheath (BS) cells. We investigated how model plant Setaria viridis responded to a four-hour or treatment at photosynthetic, transcriptomic, ultrastructural levels. Although we observed comparable reduction of photosynthetic efficiency treated...
Abstract Different intensities of high temperatures affect the growth photosynthetic cells in nature. To elucidate underlying mechanisms, we cultivated unicellular green alga Chlamydomonas reinhardtii under highly controlled photobioreactor conditions and revealed systems-wide shared unique responses to 24-hour moderate (35°C) acute (40°C) subsequent recovery at 25°C. We identified previously overlooked elements response temperature. Heat 35°C transiently arrested cell cycle followed by...
Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but of today is largely limited to gene expression analysis. Yet, the life RNA molecules multifaceted and dynamic, requiring profiling different species throughout cycle delve into intricate biology in complex tissues. Human disease-relevant tissues are commonly preserved formalin-fixed paraffin-embedded (FFPE) blocks, representing an important resource human tissue specimens. The capability...
Abstract The overexpression of human telomerase reverse transcriptase (hTERT) has been associated with the proliferation and migration colorectal cancer (CRC) cells. We investigated roles KRT23 hTERT in promoting CRC cell migration. verified relationship between using streptavidin-agarose pulldown chromatin immunoprecipitation (ChIP) assays. One hundred fifty-four specimens were analyzed immunohistochemistry. growth studied siRNA lentiviruses vivo vitro . Western blot wound scratch analyses...
Abstract Background Chronic obstructive pulmonary disease (COPD) is one of the world’s leading causes death and a major chronic disease, highly prevalent in aging population exposed to tobacco smoke airborne pollutants, which calls for early useful biomolecular predictors. Roles noncoding RNAs COPD have been proposed, however, not many studies systematically investigated crosstalk among various transcripts this context. The construction RNA functional networks such as lncRNA-mRNA,...
Neutrophils are important innate immune cells with plasticity, heterogenicity, and functional ambivalency. While bone marrow is often regarded as the primary source of neutrophil production, roles extramedullary production in regulating plasticity heterogenicity autoimmune diseases remain poorly understood. Here, we report that lack wingless-type MMTV integration site family member 5 (WNT5) unleashes anti-inflammatory protection against colitis mice, accompanied by reduced colonic CD8+ T...
PLD1 has been implicated in cytoskeletal reorganization and vesicle trafficking somatic cells; however, its function remains unclear oocyte meiosis. Herein, we found stably expresses mouse oocytes meiosis, with direct interaction spindle, RAB11A+ vesicles macroautophagic/autophagic vacuoles. The genetic or chemical inhibition of disturbed MTOC clustering, spindle assembly cortical migration, also decreased PtdIns(4,5)P2, phosphorylated CFL1 (p-CFL1 [Ser3]) ACTR2, their local distribution on...
Tumor-associated macrophages (TAMs) are key components of tumor microenvironment (TME) during tumorigenesis and progression. However, the role TAMs in lung adenocarcinoma is still unclear. In this study, we aimed to clarify mechanism underlying crosstalk between epithelial-mesenchymal transition (EMT) adenocarcinoma. Fucosyltransferase IV (FUT4) its synthetic cancer sugar antigen Lewis Y (LeY) was aberrantly elevated various solid tumors, it plays critical invasion metastasis. Here, found...
Hydrogen sulfide (H 2 S) is naturally synthesized in a wide range of mammalian tissues. Whether H S involved the regulation erythrocyte functions remains unknown. Using mice with genetic deficiency natural synthesis enzyme cystathionine‐ γ ‐lyase (CSE) and high‐throughput metabolomic profiling, we found that levels 2,3‐bisphosphoglycerate (2,3‐BPG), an erythroid‐specific metabolite negatively regulating hemoglobin‐ (Hb‐) oxygen (O ) binding affinity, were increased CSE knockout ( Cse -/-...
Hypertrophic scar (HS), a fibroproliferative disorder caused by abnormal wound healing after skin injury, which is characterized excessive deposition of extracellular matrix and invasive growth fibroblasts. Recent studies have shown that some non-coding RNA implicated the formation HS, but mechanism remains unclear. In this study, we found lncRNA TRHDE-AS1 was downregulated in HS tissues HSFs, level negatively correlated with miR-181a-5p tissue HSFs. Overexpressed significantly suppressed...
The unicellular green alga
Summary The key technical bottleneck for exploiting plant hairy root cultures as a robust bioproduction platform therapeutic proteins has been low protein productivity, particularly secreted yields. To address this, we engineered novel hydroxyproline (Hyp)‐ O ‐glycosylated peptides (Hyp GP s) into tobacco roots to boost the extracellular secretion of fused and elucidate Hyp‐ ‐glycosylation process cell wall Hyp‐rich glycoproteins. Hyp s representing two major types glycoproteins were...
Expression of recombinant proteins fused to a novel glycomodule tag, termed hydroxyproline (Hyp)-O-glycosylated peptides (HypGP), was earlier found boost secreted protein yields up 500-fold in plant cell culture. Here, this technology applied the expression human protease inhibitor α1-antitrypsin (AAT) tobacco BY-2 A designer HypGP tag composed 'Ala-Pro' motif 20 units, or (AP)20, engineered either at N- C-terminal end AAT. The (AP)20 substantially increased AAT 34.7 mg/L. However,...
Drought is a critical abiotic stress that influences maize yield and reduces grain when it occurs at the flowering or filling stage. To dissect genetic architecture of under drought (DS), genome-wide association analysis was conducted in population composed diverse inbred lines from five locations well-watered DS conditions 2019 2020.Using fixed random model circulating probability unification model, total 147 loci associated with resistance index (DRI) were identified, which 54 DRI an...