- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Genetic and phenotypic traits in livestock
- Crop Yield and Soil Fertility
- Wheat and Barley Genetics and Pathology
- Plant nutrient uptake and metabolism
- Antioxidant Activity and Oxidative Stress
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Food composition and properties
- Rice Cultivation and Yield Improvement
- Viral gastroenteritis research and epidemiology
- Silkworms and Sericulture Research
- Lipid metabolism and biosynthesis
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Malaria Research and Control
- Zoonotic diseases and public health
- RNA modifications and cancer
- Polysaccharides Composition and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Community Ecology and Physiology
- Plant responses to elevated CO2
- RNA Research and Splicing
Ministry of Agriculture and Rural Affairs
2019-2024
Northwest A&F University
2014-2024
China Agricultural University
2009-2024
Jiangsu Institute of Parasitic Diseases
2019-2024
Shandong Management University
2024
Central Cotton Research Institute
2022
Shaanxi Science and Technology Department
2018-2021
Shandong Provincial QianFoShan Hospital
2020
State Key Laboratory of Plant Physiology and Biochemistry
2019
Sichuan University
2013
Tocopherols are a class of four natural compounds that can provide nutrition and function as antioxidant in both plants animals. Maize kernels have low α-tocopherol content, the compound with highest vitamin E activity, thus, raising risk deficiency human populations relying on maize their primary source. In this study, two insertion/deletions (InDels) within gene encoding γ-tocopherol methyltransferase, Zea mays VTE4 (ZmVTE4), single nucleotide polymorphism (SNP) located ∼85 kb upstream...
Starch is the main storage carbohydrate in plants and an important natural resource for food, feed industrial raw materials. However, details regarding pathway starch biosynthesis diversity of biosynthetic enzymes involved this process are poorly understood. This study uses a comprehensive phylogenetic analysis 74 sequenced plant genomes to revisit evolutionary history genes encoding ADP-glucose pyrophosphorylase (AGPase), synthase (SS), branching enzyme (SBE) de-branching (DBE)....
Abstract Mapping quantitative trait loci in outbred populations is important because many of organisms are noninbred. Unfortunately, information about the genetic architecture may not be available populations. Thus, allelic effects genes can estimated with ease. In addition, under linkage equilibrium, marker genotypes provide no genotype a QTL (our terminology for single locus while multiple referred to as QTLs). To circumvent this problem, an interval mapping procedure based on random model...
Soil waterlogging is a major environmental stress that suppresses maize (Zea mays) growth and yield. To identify quantitative trait loci (QTL) associated with tolerance at the seedling stage, F2 population consisting of 288 F2:3 lines was created from cross between two genotypes, 'HZ32' (waterlogging-tolerant) 'K12' (waterlogging-sensitive). The genotyped base-map 1710·5 cM length constructed an average marker space 11·5 based on 177 SSR (simple sequence repeat) markers. QTL root length, dry...
Summary Meiotic recombination is a major driver of genetic diversity, species evolution, and agricultural improvement. Thus, an understanding the landscape across maize ( Zea mays ) genome will provide insight tools for further study evolution Here, we used c . 50 000 single nucleotide polymorphisms to precisely map events in 12 artificial segregating populations. We observed substantial variation frequency distribution along ten chromosomes among populations identified 143 hot regions....
Abstract Maize is one of China's most important grain crops. We analysed data from breeding trials and experimental reports to assess genetic gains in the single‐cross varieties terms yield agronomic traits Northern China, Southwestern China Yellow–Huai River Valley. Since 1960s, has increased linearly all three regions. Plant height, ear rows 100‐seed weight have (P < 0.05) with time, while length decreased 0.05). Planting density Valley but no change China. Ear height leaf number over...
Although tocopherols play an important role in plants and animals, the genetic architecture of tocopherol content maize kernels has remained largely unknown. In this study, linkage association analyses were conducted to examine kernels. Forty-one unique quantitative trait loci (QTLs) identified by mapping six populations recombinant inbred lines (RILs). addition, 32 significant detected via genome-wide study (GWAS), 18 which colocalized with QTLs mapping. Fine a major QTL validated accuracy...
Plant height (PH) and ear (EH) are key plant architectural traits in maize, which will affect the photosynthetic efficiency, high density tolerance, suitability for mechanical harvesting. QTL mapping were conducted PH EH using a recombinant inbred line (RIL) population two corresponding immortalized backcross (IB) populations obtained from crosses between RIL parental lines. A total of 17 15 detected IB populations, respectively. Two QTL, qPH1-1 (qEH1-1) qPH1-2 (qEH1-4) RIL, simultaneously...
This `Letter to the Editor` concerns difference between using a simple regression method for mapping quantitative trait loci (QTLs) and maximum likelihood method. The article discusses basic concepts analyses of data from simulated backcross population size 1000. 7 refs., 1 tab.
Abstract α‐carotene is one of the important components pro‐vitamin A, which able to be converted into vitamin A in human body. One maize ( Zea mays L.) ortholog carotenoid hydroxylases Arabidopsis thaliana , ZmcrtRB3 was cloned and its role hydrolyzations addressed. mapped a quantitative trait locus (QTL) cluster for carotenoid‐related traits on chromosome 2 (bin 2.03) recombinant inbred line (RIL) population derived from By804 B73. Candidate‐gene association analysis identified 18...
Abstract Background Tocopherols, which are vitamin E compounds, play an important role in maintaining human health. Compared with other staple foods, maize grains contain high level of tocopherols. Results Two F 2 populations (K22/CI7 and K22/Dan340, referred to as POP-1 POP-2, respectively), share a common parent (K22), were developed genotyped using GoldenGate assay containing 1,536 single nucleotide polymorphism (SNP) markers. An integrated genetic linkage map was constructed 619 SNP...
The endosperm is a major organ of the seed that plays vital roles in determining weight and quality. However, genome-wide transcriptome patterns throughout maize development have not been comprehensively investigated to date. Accordingly, we performed high-throughput RNA sequencing (RNA-seq) analysis at 5, 10, 15 20 days after pollination (DAP). We found more than 11,000 protein-coding genes underwent alternative splicing (AS) events during four developmental stages studied. These were...
Knowledge of the transcription factor binding landscape (TFBL) is necessary to analyze gene regulatory networks for important agronomic traits. However, a low-cost and high-throughput in vivo chromatin profiling method still lacking plants. Here, we developed transient simplified cleavage under targets tagmentation (tsCUT&Tag) that combines expression proteins protoplasts with CUT&Tag without nucleus extraction. Our tsCUT&Tag provided higher data quality signal resolution lower sequencing...
Genome-wide prediction is a promising approach to boost selection gain in hybrid breeding. Our main objective was evaluate the potential and limits of genome-wide identify superior combinations adapted Northwest China. A total 490 hybrids derived from crosses among 119 inbred lines Shaan B heterotic pattern were used for ten agronomic traits. We tested eight different statistical models considering additive (A) effects addition evaluated impact dominance (D) epistasis (E) on ability....
Abstract Background Kernel dehydration is an important factor for the mechanized harvest in maize. moisture content (KMC) and kernel rate (KDR) are indicators dehydration. Although quantitative trait loci genes related to KMC have been identified, where most of them only focus on at harvest, these still far from sufficient explain all genetic variations, relevant regulatory mechanisms unclear. In this study, we tried reveal key proteins metabolites proteome metabolome levels. Moreover,...
Maize kernel moisture content (KMC) at harvest greatly affects mechanical harvesting, transport and storage. KMC is correlated with dehydration rate (KDR) before after physiological maturity. KDR are complex traits governed by multiple quantitative trait loci (QTL). Their genetic architecture incompletely understood. We used a multiomics integration approach an association panel to identify genes influencing KDR. A genome-wide study using time-series data from 7 70 days pollination their...
Increasing grain yield is a primary objective of maize breeding. Dissecting the genetic architecture furthers improvements to increase yield. Presented here an association panel composed 126 inbreds (AM126), which were genotyped by genotyping-by-sequencing (tGBS) method. We performed characterization and analysis related in panel. In total, 46,046 SNPs with minor allele frequency (MAF) ≥0.01 used assess diversity kinship AM126. The results showed that average MAF polymorphism information...
Abstract Particle‐size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological nutritional function of starch. Flow cytometry flow sorting are widely considered convenient efficient ways classifying separating natural biological particles or other substances into subpopulations, respectively, based on differential response each component to stimulation by a light beam; results allow for correlation analysis parameters. In this study,...
In this study, we established the DDP-resistant NSCLC cell line A549/DDP to detect effect of NORAD on cisplatin resistance cells.NORAD was highly expressed in cells compared with A549 cells.The MTT data showed that knockdown enhanced inhibition rate and decreased IC50 value.The colony formation assay suggested inhibited proliferation, inhibitory cells.Besides, found silence reduced P-gp expression but not BCRP, LRP MRP.Moreover, could directly bind miR-202-5p, ABCB1 a target miR-202-5p.The...