A5100356107- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Molecular Sensors and Ion Detection
- Anesthesia and Neurotoxicity Research
- Nanoparticle-Based Drug Delivery
- Molecular Biology Techniques and Applications
- Supramolecular Chemistry and Complexes
- Cell Image Analysis Techniques
- Gene expression and cancer classification
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Luminescence and Fluorescent Materials
- Sulfur Compounds in Biology
- RNA Research and Splicing
- CRISPR and Genetic Engineering
Universität Hamburg
2022-2025
Jilin University
2014-2025
Union Hospital
2025
Yale University
2002-2024
University of Illinois System
2015-2024
Oak Ridge National Laboratory
2024
Guangxi Medical University
2024
North Sichuan Medical University
2024
Affiliated Hospital of North Sichuan Medical College
2024
First Affiliated Hospital of GuangXi Medical University
2024
Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, have epitope-tagged 60% Saccharomyces cerevisiae proteome. By high-throughput immunolocalization tagged gene products, determined subcellular 2744 yeast proteins. Extrapolating these through computational...
Spatial omics emerged as a new frontier of biological and biomedical research. Here, we present spatial-CUT&Tag for spatially resolved genome-wide profiling histone modifications by combining in situ CUT&Tag chemistry, microfluidic deterministic barcoding, next-generation sequencing. Spatially chromatin states mouse embryos revealed tissue-type-specific epigenetic regulations concordance with ENCODE references provide spatial information at tissue scale. Spatial-CUT&Tag control the cortical...
Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells context
The hippocampal-entorhinal system supports cognitive functions, has lifelong neurogenic capabilities in many species, and is selectively vulnerable to Alzheimer's disease. To investigate potential cellular diversity, we profiled single-nucleus transcriptomes five subregions humans, macaques, pigs. Integrated cross-species analysis revealed robust transcriptomic histologic signatures of neurogenesis the adult mouse, pig, macaque but not humans. Doublecortin (DCX), a widely accepted marker...
Abstract Emerging spatial technologies, including transcriptomics and epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context 1–5 . However, current methods capture only one layer omics information at a time, precluding possibility examining mechanistic relationship across central dogma molecular biology. Here, we present two technologies spatially resolved, genome-wide, joint epigenome transcriptome by cosequencing chromatin accessibility gene...
Abstract In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension demonstrated high-plex protein whole transcriptome co-mapping. We profiled 189 proteins in multiple mouse tissue types with CITE sequencing then further applied method measure 273 human tissues, revealing spatially distinct germinal center reactions tonsil early immune activation skin at Coronavirus Disease 2019 mRNA vaccine injection site.
The capability to spatially explore RNA biology in formalin-fixed paraffin-embedded (FFPE) tissues holds transformative potential for histopathology research. Here, we present pathology-compatible deterministic barcoding tissue (Patho-DBiT) by combining situ polyadenylation and computational innovation spatial whole transcriptome sequencing, tailored probe the diverse species clinically archived FFPE samples. It permits co-profiling of gene expression processing, unveiling region-specific...
Plants are complex systems hierarchically organized and composed of various cell types. To understand the molecular underpinnings plant systems, single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for revealing high resolution gene expression patterns at cellular level investigating cell-type heterogeneity. Furthermore, scRNA-seq analysis biosystems great potential generating new knowledge to inform design synthetic biology, which aims modify plants...
Background Small molecules have been shown to modulate the neurogenesis processes. In search for new therapeutic drugs, herbs used in traditional medicines are promising candidates. Methodology and Principal Findings We selected a total of 45 natural compounds from Traditional Chinese herbal which extensively China treat stroke clinically, tested their proliferation-inducing activities on neural stem/progenitor cells (NSPCs). The screening results showed that salvianolic acid B (Sal B)...
Exosomes play important roles in mediating intercellular communication and regulating a variety of biological processes, but clear understanding their functions biogenesis has not been achieved, due to the high technical difficulties involved analysis small vesicular structures that contain proportion membrane structures. Herein, we designed novel approach integrate two nanomaterials carrying varied surface properties, hydrophilic, macroporous graphene foam (GF) amphiphilic periodic...
A dual-mode aggregative host:guest indicator displacement sensing system has been created for the detection of trimethylated peptides and determination histone demethylase activity. The combination selective recognition suitably sized trimethylammonium salts reversible lipophilic aggregation complex provides a unique quenching mechanism that is not only dependent on affinity sensitivity but lipophilicity indicator. In addition, can be controlled by application chaotropic anions in mixture,...
Arrayed deep cavitands can be coupled to a fluorescence-based supramolecular tandem assay that allows site-selective in situ monitoring of post-translational modifications catalyzed by the lysine methyltransferase PRDM9 or demethylase JMJD2E. An arrayed sensor system containing only three cavitand components detect specific substrates enzyme modification, presence other histone peptides assay, enabling investigation cross-reactivity over multiple methylation sites and interference from...
This protocol describes the use of deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen samples. approach uses microfluidic-based method introduce combinatorial DNA oligo barcodes directly cells section glass slide. technique does not resolve single but can achieve near-single-cell resolution transcriptomics and analysis targeted panel proteins. For complete details execution this protocol, please refer Liu et al. (2020).
Abstract Hippocampal circuitry stimulation is sufficient to regulate adult hippocampal neurogenesis and ameliorate depressive‐like behavior, but its underlying mechanism remains unclear. Here, it shown that inhibition of medial septum (MS)‐dentate gyrus (DG) circuit reverses the chronic social defeat stress (CSDS)‐induced depression‐like behavior. Further analysis exhibits gamma‐aminobutyric acidergic neurons in MS projecting DG (MS GABA+ ‐DG) increases expression platelet‐derived growth...
Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but of today is largely limited to gene expression analysis. Yet, the life RNA molecules multifaceted and dynamic, requiring profiling different species throughout cycle delve into intricate biology in complex tissues. Human disease-relevant tissues are commonly preserved formalin-fixed paraffin-embedded (FFPE) blocks, representing an important resource human tissue specimens. The capability...
A fluorogenic probe with mitochondria targeting capability was prepared for detection of superoxide radical generation inside in living cells.
Simple tuning of a host:guest pair allows selective sensing different peptide modifications, exploiting orthogonal recognition mechanisms. Excellent selectivity for either lysine trimethylations or alcohol phosphorylations is possible by simply varying the fluorophore guest. The phosphorylation sensor can be modulated presence small (μM) concentrations metal ions, allowing array-based sensing. Phosphorylation at serine, threonine, and tyrosine selectively sensed via discriminant analysis....
Concern over potential neurotoxicity of anesthetics has led to growing interest in prospective clinical trials using potentially less toxic anesthetic regimens, especially for prolonged anesthesia infants. Preclinical studies suggest that dexmedetomidine may have a reduced neurotoxic profile compared other conventional regimens; however, coadministration with either drugs (eg, remifentanil) and/or regional blockade is required achieve adequate surgery. The feasibility this pharmacological...
Background Latent tuberculosis infection affects about one-quarter of the global population and can progress to active tuberculosis. Hematological inflammatory markers, such as systemic immune-inflammation index, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte monocyte-to-lymphocyte reflect inflammation immune status but are understudied in latent infection. This study investigates association between these markers a nationally representative sample. Methods Data from 7,042...