A5063552519- Chromosomal and Genetic Variations
- SARS-CoV-2 and COVID-19 Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Reproductive Biology and Fertility
- Plant tissue culture and regeneration
- Pregnancy and preeclampsia studies
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Reproductive System and Pregnancy
- Animal Virus Infections Studies
- Ovarian function and disorders
- Immune Cell Function and Interaction
- Wheat and Barley Genetics and Pathology
- Cancer-related molecular mechanisms research
- Plant biochemistry and biosynthesis
- Multiple Myeloma Research and Treatments
- Single-cell and spatial transcriptomics
- Assisted Reproductive Technology and Twin Pregnancy
- Immunotherapy and Immune Responses
- Chronic Myeloid Leukemia Treatments
Yale University
2024-2025
Syngenta (China)
2025
Fudan University
2020-2024
Hubei University of Arts and Science
2018-2024
Army Medical University
2024
Xiangyang Central Hospital
2018-2024
Chongqing Cancer Hospital
2024
Chongqing University
2024
China University of Mining and Technology
2024
Guizhou University
2020-2023
The newly identified 2019 novel coronavirus (2019-nCoV) has caused more than 11,900 laboratory-confirmed human infections, including 259 deaths, posing a serious threat to health. Currently, however, there is no specific antiviral treatment or vaccine. Considering the relatively high identity of receptor-binding domain (RBD) in 2019-nCoV and SARS-CoV, it urgent assess cross-reactivity anti-SARS CoV antibodies with spike protein, which could have important implications for rapid development...
ARS-CoV-2 outbreak is a world-wide pandemic. The Spike protein plays central role in cell entry of the virus, and triggers significant immuno-response. Our understanding immune-response against S still very limited. Herein, we constructed peptide microarray analyzed 55 convalescent sera, three areas with rich linear epitopes were identified. Potent neutralizing antibodies enriched from sera by 3 peptides, which do not belong to RBD revealed.
The capability to spatially explore RNA biology in formalin-fixed paraffin-embedded (FFPE) tissues holds transformative potential for histopathology research. Here, we present pathology-compatible deterministic barcoding tissue (Patho-DBiT) by combining situ polyadenylation and computational innovation spatial whole transcriptome sequencing, tailored probe the diverse species clinically archived FFPE samples. It permits co-profiling of gene expression processing, unveiling region-specific...
ABSTRACT The newly identified 2019 novel coronavirus (2019-nCoV) has caused more than 800 laboratory-confirmed human infections, including 25 deaths, posing a serious threat to health. Currently, however, there is no specific antiviral treatment or vaccine. Considering the relatively high identity of receptor binding domain (RBD) in 2019-nCoV and SARS-CoV, it urgent assess cross-reactivity anti-SARS-CoV antibodies with spike protein, which could have important implications for rapid...
Perturb-seq enabled the profiling of transcriptional effects genetic perturbations in single cells but lacks ability to examine impact on tissue environments. We present Perturb-DBiT for simultaneous co- sequencing spatial transcriptome and guide RNAs (gRNAs) same section vivo CRISPR screen with genome-scale gRNA libraries, offering a comprehensive understanding how modifications affect cellular behavior architecture. This platform supports variety delivery vectors, library sizes,...
Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but of today is largely limited to gene expression analysis. Yet, the life RNA molecules multifaceted and dynamic, requiring profiling different species throughout cycle delve into intricate biology in complex tissues. Human disease-relevant tissues are commonly preserved formalin-fixed paraffin-embedded (FFPE) blocks, representing an important resource human tissue specimens. The capability...
The current COVID-19 pandemic has heavily burdened the global public health system and may keep simmering for years. frequent emergence of immune escape variants have spurred search prophylactic vaccines therapeutic antibodies that confer broad protection against SARS-CoV-2 variants. Here we show bivalency an affinity maturated fully human single-domain antibody (n3113.1-Fc) exhibits exquisite neutralizing potency pseudovirus, confers effective authentic in host cell receptor...
The continuous emergence of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants poses new challenges in the fight against disease 2019 (COVID-19) pandemic. newly emerging Omicron strain caused serious immune escape and raised unprecedented concern all over world. development an antibody targeting a conserved universal epitope is urgently needed. A subset neutralizing antibodies (NAbs) COVID-19 from convalescent patients were isolated our previous study. In this study,...
Dengue is the most widespread vector-borne viral disease caused by dengue virus (DENV) for which there are no safe, effective drugs approved clinical use. Here, using sequential antigen panning of a yeast antibody library derived from healthy donors against DENV envelop protein domain III (DIII) combined with depletion an entry defective DIII mutant, we identified cross-reactive human monoclonal (mAb), m366.6, bound high affinity to all four serotypes. Immunogenetic analysis indicated that...
In vivo doubled-haploid technology is widely applied in commercial maize breeding programs because of its time-saving and cost-reducing features. The production haploids primarily depends on the use Stock6-derived haploid inducer lines. Although gene underlying induction, MTL/ZmPLA1/NLD, was cloned recently, mechanism induction still unknown. Hetero-fertilization can occur via a single fertilization, which provides means to investigate single-fertilization events by studying...
The pleiotropic effects of human disease and the complex nature gene-interaction networks require knock-in mice allowing for multiplexed gene perturbations. Here we describe a series with C57BL/6 background conditional or constitutive expression LbCas12a high-fidelity enhanced AsCas12a, which were inserted at Rosa26 locus. Cas12a in did not lead to discernible pathology enabled efficient genome engineering. We used retrovirus-based immune-cell engineering CD4+ CD8+ T cells, B cells...