A5064608517- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Ocular Infections and Treatments
- Allergic Rhinitis and Sensitization
- Intraocular Surgery and Lenses
- Ophthalmology and Visual Impairment Studies
- Retinal Diseases and Treatments
- RNA Interference and Gene Delivery
- Ocular Diseases and Behçet’s Syndrome
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- Optical Coherence Tomography Applications
- Retinal Imaging and Analysis
- Nasolacrimal Duct Obstruction Treatments
- Immune Response and Inflammation
- Air Quality and Health Impacts
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Advanced Nanomaterials in Catalysis
- RNA modifications and cancer
- interferon and immune responses
Fudan University
2015-2025
Eye & ENT Hospital of Fudan University
2015-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2025
Shanghai Eye Disease Prevention & Treatment Center
2008-2025
State Key Laboratory of Synthetic Chemistry
2023-2025
Children's Hospital of Fudan University
2024-2025
State Key Laboratory of Medical Neurobiology
2024-2025
Guiyang Medical University
2018-2024
Affiliated Hospital of Guizhou Medical University
2018-2024
Shanghai University
2024
Tissue-specific delivery of oligonucleotide therapeutics beyond the liver remains a key challenge in nucleic acid drug development. To address this issue, exploiting exosomes as novel carrier has emerged promising approach for efficient delivery. However, current exosome-based systems still face multiple hurdles their clinical applications. Herein, work presents strategy constructing hybrid exosome vehicle (HEV) through DNA zipper-mediated membrane fusion tissue-specific siRNA As...
In vivo CRISPR gene editing holds enormous potential for various diseases. Ideally, delivery should be cell type-specific and time-restricted optimal efficacy safety, but customizable methods are lacking. Here we develop a cell-tropism programmable CRISPR-Cas9 ribonucleoprotein system (RIDE) based on virus-like particles. The efficiency of RIDE was comparable to that adeno-associated virus lentiviral vectors higher than lipid nanoparticles. could readily reprogrammed target dendritic cells,...
To compare intraocular pressure (IOP) measurements obtained using the Topocon noncontact tonometer (NCT), Goldmann applanation (GAT), and Corvis ST (CST), a newly developed with features of visualization measurement corneal deformation response to an air impulse. A secondary objective was assess agreement among devices.Fifty-nine participants, including glaucoma patients (36 cases) control volunteers (23 cases), were enrolled. One eye selected randomly for further study. IOP CST, NCT, GAT by...
To investigate the applicability of a newly developed corneal topographer in assessing tear film stability.This is prospective, case-control study. Forty-four Chinese dry eye patients and 41 normal subjects were recruited. Noninvasive break-up time (NI-BUT) was measured using new method based on equipped with modified scan software. The reliability measurements determined. Then, correlations between NI-BUT traditional fluorescein time, Schirmer I test values, inferior meniscus height...
Abstract Background Dry eye syndrome (DES) is a common ocular disorder occurring in general population. The purpose of this study to evaluate the impact DES on vision-related quality life (QoL) non-clinic-based Methods This population-based cross-sectional enrolled subjects older than 40 years, who took part an epidemiological dry Sanle Community, Shanghai. Apart from collection sociodemographics, symptoms, and other clinical data, Chinese version 25-item National Eye Institute Visual...
Purpose To investigate the features of corneal epithelial thickness topography with Fourier-domain optical coherence tomography (OCT) in dry eye patients. Methods In this cross-sectional study, 100 symptomatic patients and 35 normal subjects were enrolled. All participants answered ocular surface disease index questionnaire subjected to OCT, fluorescein staining, tear breakup time, Schirmer 1 test without anesthetic (S1t), meibomian morphology. Several epithelium statistics for each eye,...
Abstract Allergic conjunctivitis is a common problem that significantly impairs patients’ quality of life. Whether air pollution serves as risk factor for the development allergic remains elusive. In this paper, we assess relationship between pollutants and weather conditions with outpatient visits conjunctivitis. By using time-series analysis based on largest dataset ever assembled to date, found number was correlated levels NO 2 , O 3 temperature, while its association humidity...
To demonstrate the identification of corneal diseases using a novel deep learning algorithm. A hierarchical network, which is composed family multi-task multi-label classifiers representing different levels eye derived from predefined disease taxonomy was designed. Next, we proposed multi-level disease-guided loss function to learn fine-grained variability features. The algorithm trained end-to-end directly 5,325 ocular surface images retrospective dataset. Finally, algorithm's performance...
Abstract The therapeutic potential of nanomaterials toward oxidative damage relevant diseases has attracted great attentions by offering promising advantages compared with conventional antioxidants. Although different kinds nanoantioxidants have been well developed, the facile fabrication robust and efficient nanoscavengers is still met challenges like use toxic high‐cost subunits, involvement multistep synthetic process, redundant purification work. Herein, a direct strategy polyphenol...
Protein degradation techniques, such as proteolysis-targeting chimeras (PROTACs) and lysosome-targeting (LYTACs), have emerged promising therapeutic strategies for the treatment of diseases. However, efficacy current protein methods still needs to be improved address complex mechanisms underlying Herein, a LYTAC Plus hydrogel engineered is proposed by nucleic acid self-assembly, which integrates gene silencing motif into construct enhance its potential. As proof-of-concept study, vascular...
The design of intracellular delivery systems for protein drugs remains a challenge due to limited efficacy and serum stability. Herein, we propose reversible assembly strategy assemble cargo proteins phenolic polymers into stable nanoparticles this purpose using heterobifunctional adaptor (2-formylbenzeneboronic acid). is easily decorated on via iminoboronate chemistry further conjugates with catechol-bearing form boronate diester linkages. exhibit excellent stability in culture media but...
To measure corneal and limbal epithelial thickness (ET) in normal subjects to evaluate its variation with age by using anterior segment optical coherence tomography (AS-OCT).A total of 180 (180 healthy eyes) were enrolled divided into four groups according age: A (0-20 years), B (21-40 C (41-60 D (>60 years). Cornea limbus imaged OCT. Corneal ET (CET) was obtained automatically the built-in analysis software OCT system. Limbal (LET) quadrants manually measured from images.Corneal a central...
To determine the validity and reliability of clinical assessment bulbar redness (BR) using a newly developed corneal topographer.This is cross-sectional diagnostic evaluation study. The BR scores [Oculus Index (OI)] from 30 eyes 26 patients with different degrees conjunctival hyperemia were assessed scored automatically new method: keratograph equipped scanning scoring software. values obtained via this system correlated three image-based comparative subjective scales: Institute for Eye...
Bacterial keratitis is the most common corneal infection which may lead to blindness, and seriously threatened human visual health worldwide. Clinical treatment with antibiotic eye drops formulation usually falls in low bioavailability poor therapeutic efficiency. Hydrogel has gained much attention as ophthalmic recently due prolonged drug retention on ocular surface. In this study, we proposed a type of all-small-molecule supramolecular hydrogel assembled from guanosine-5'-monophosphate...
Current vehicles used to deliver antisense oligonucleotides (ASOs) cannot distinguish between bacterial and mammalian cells, greatly hindering the preclinical or clinical treatment of infections, especially those caused by antibiotic-resistant bacteria. Herein, bacteria-specific ATP-binding cassette (ABC) sugar transporters are leveraged selectively internalize ASOs hitchhiking them on α (1-4)-glucosidically linked glucose polymers. Compared with their cell-penetrating peptide counterparts,...
Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 ,...
<h3>Aims</h3> To analyse age-related changes of human conjunctiva by in vivo laser scanning confocal microscopy (LSCM). <h3>Methods</h3> 80 healthy subjects were enrolled and divided into four groups according to age: A (0–19 years), B (20–39 C (40–59 years) D (over 60 years). In LSCM was performed on the subjects9 bulbar conjunctivas, images recorded. The morphology densities conjunctival epithelial cells, goblet cells (GCs), dendritic (DCs) positive rate microcysts analysed. diameters...