A5084856814- Ocular Surface and Contact Lens
- Nicotinic Acetylcholine Receptors Study
- Retinal Development and Disorders
- Neuroscience of respiration and sleep
- Glaucoma and retinal disorders
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Corneal Surgery and Treatments
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Advanced Nanomaterials in Catalysis
- Sleep and Wakefulness Research
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Facial Rejuvenation and Surgery Techniques
- Protein purification and stability
- Asthma and respiratory diseases
- Peripheral Neuropathies and Disorders
- Allergic Rhinitis and Sensitization
- Artificial Intelligence in Healthcare and Education
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Immune Response and Inflammation
- Multiple Sclerosis Research Studies
- Advanced biosensing and bioanalysis techniques
Eye & ENT Hospital of Fudan University
2016-2025
Fudan University
2017-2025
State Key Laboratory of Medical Neurobiology
2009-2025
Shanghai Eye Disease Prevention & Treatment Center
2024-2025
Children's Hospital of Fudan University
2024
State Key Laboratory of Synthetic Chemistry
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2023
Center for High Pressure Science & Technology Advanced Research
2020
Shanghai Medical College of Fudan University
1992-2017
Science and Technology Commission of Shanghai Municipality
2017
Tissue-specific delivery of oligonucleotide therapeutics beyond the liver remains a key challenge in nucleic acid drug development. To address this issue, exploiting exosomes as novel carrier has emerged promising approach for efficient delivery. However, current exosome-based systems still face multiple hurdles their clinical applications. Herein, work presents strategy constructing hybrid exosome vehicle (HEV) through DNA zipper-mediated membrane fusion tissue-specific siRNA As...
In vivo CRISPR gene editing holds enormous potential for various diseases. Ideally, delivery should be cell type-specific and time-restricted optimal efficacy safety, but customizable methods are lacking. Here we develop a cell-tropism programmable CRISPR-Cas9 ribonucleoprotein system (RIDE) based on virus-like particles. The efficiency of RIDE was comparable to that adeno-associated virus lentiviral vectors higher than lipid nanoparticles. could readily reprogrammed target dendritic cells,...
Abstract The therapeutic potential of nanomaterials toward oxidative damage relevant diseases has attracted great attentions by offering promising advantages compared with conventional antioxidants. Although different kinds nanoantioxidants have been well developed, the facile fabrication robust and efficient nanoscavengers is still met challenges like use toxic high‐cost subunits, involvement multistep synthetic process, redundant purification work. Herein, a direct strategy polyphenol...
Protein degradation techniques, such as proteolysis-targeting chimeras (PROTACs) and lysosome-targeting (LYTACs), have emerged promising therapeutic strategies for the treatment of diseases. However, efficacy current protein methods still needs to be improved address complex mechanisms underlying Herein, a LYTAC Plus hydrogel engineered is proposed by nucleic acid self-assembly, which integrates gene silencing motif into construct enhance its potential. As proof-of-concept study, vascular...
The design of intracellular delivery systems for protein drugs remains a challenge due to limited efficacy and serum stability. Herein, we propose reversible assembly strategy assemble cargo proteins phenolic polymers into stable nanoparticles this purpose using heterobifunctional adaptor (2-formylbenzeneboronic acid). is easily decorated on via iminoboronate chemistry further conjugates with catechol-bearing form boronate diester linkages. exhibit excellent stability in culture media but...
Nanoparticle-based mRNA delivery offers a versatile platform for innovative therapies. However, most of the current systems are limited by poor serum tolerance, suboptimal endosomal escape and efficacy. Herein, highly efficient mRNA-delivering material is identified from library fluoropolymers. The lead FD17 shows exceptional stability escape, enabling into various cell types, surpassing commercial reagents such as Lipofectamine 3000. formed nanoparticles adsorb abundant albumin on surface,...
Glaucoma is a multifactorial, neurodegenerative disorder characterized by the loss of retinal ganglion cells (RGCs). Crosstalk between gut microbiota and host involved in progression many diseases, although little known about its role glaucoma. To investigated alterations derived metabolites glaucomatous rats, interaction with RGCs, we performed 16S rRNA (V1-V9) sequencing untargeted metabolomic analyses. The microbial composition differed significantly two groups, diversity cecal bacteria...
Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 ,...
Serotonin (5-hydroxytryptamine, 5-HT) receptor agonists are neuroprotective in CNS injury models. However, the functional implications and synaptic mechanism of 8-hydroxy-2- (di-n-propylamino) tetralin (8-OH-DPAT), a serotonin (5-HT1A) agonist, an adult male Wistar rat model chronic glaucoma remain unknown. We found that ocular hypertension decreased 5-HT1A expression retinas because number retinal ganglion cells (RGCs) was significantly reduced rats with induced relative to control...
To evaluate the quality of responses from large language models (LLMs) to patient-generated conjunctivitis questions.
Glaucoma is a progressive neuropathy characterized by the loss of retinal ganglion cells (RGCs). Strategies that delay or halt RGC have been recognized as potentially beneficial for rescuing vision in glaucoma patients. Quercetin natural and important dietary flavonoid compound, widely distributed fruits vegetables. Mounting evidence suggests quercetin (Qcn) has numerous neuroprotective effects. However, whether Qcn exerts effects on poorly understood. In this study, we investigated...
The aim of this study was to investigate the role and mechanism berberine (BBR) in protection injured retinal ganglion cells (RGCs) diabetic retinopathy (DR).Experimental rat model successfully induced by a single intraperitoneal injection streptozotocin (STZ, 60 mg/kg) male SD rats with sufficient food water for 8 weeks. Animals were randomly divided into four groups: (1) non-diabetic, (2) diabetic, (3) + BBR PBS, (4) SR95531. (100 given daily gavage group weeks, weekly intravitreal...
Abstract Dry eye disease (DED) is a growing public health concern affecting millions of people worldwide and causing ocular discomfort visual disturbance. Developing its therapeutic drugs based on animal models suffer from interspecies differences poor prediction human trials. Here, we established long‐term 3D corneal epithelial organoids, which recapitulated the cell lineages gene expression signature epithelium. Organoids can be regulated to differentiate ex vivo, but addition FGF10...
To evaluate levels of 3 tear-soluble neuropeptides in dry eye patients and to identify the correlations with clinical signs symptoms. A total 16 12 healthy volunteers were enrolled. Dry disease (DED) diagnosis was based on 2017 Report Tear Film & Ocular Surface Society International Eye Workshop (TFOS DEWS II). First time noninvasive breakup (NIBUT-1st), mean (NIBUT-avg), tear meniscus height (TMH), Schirmer test, corneal fluorescein staining (CFS) score ocular surface index (OSDI) recorded....
Patients with dry eye disease (DED) often exhibit neurological abnormalities and may even suffer from neuropathic pain pain-related anxiety or depression. The α-7 nicotinic acetylcholine receptor (α7nAChR) is a pivotal regulator in the anti-inflammatory pathway connecting nervous immune systems, Here, we investigate potential of α7nAChR agonist as novel treatment for DED. We induced DED model by unilateral excision extraorbital lachrymal gland C57/BL6 mice. After seven days treatment, RNA...
Alpha-7 nicotinic acetylcholine receptor (α7-nAChR) agonists can prevent glutamate-induced excitotoxicity in cultured retinal ganglion cells (RGCs). However, the neuroprotective effects and mechanism of action PNU-282987, an α7-nAChR agonist, a chronic vivo rat glaucoma model are poorly understood. We found that elevated intraocular pressure (IOP) downregulated expression. Electroretinography revealed amplitude photopic negative response (PhNR) decreased parallel with loss RGCs caused by...
Glaucoma is a neurodegenerative disease caused by excitotoxic injury of retinal ganglion cells (RGCs). In our previous model high intraocular pressure, prepared injecting magnetic beads into the anterior chamber, we demonstrated that an important natural dietary flavonoid compound (quercetin) can improve RGC function. However, it unclear whether quercetin synaptic function RGCs and how regulates transmission in rat models chronic glaucoma.A glaucoma was electrocoagulation superior scleral...
Presynaptic modulation of γ-aminobutyric acid (GABA) release by an alpha7 nicotinic acetylcholine receptor (α7-nAChR) agonist promotes retinal ganglion cell (RGC) survival and function, as suggested a previous study on chronic glaucomatous model from our laboratory. However, the role excitatory inhibitory amino receptors their interaction with α7-nAChR in physiological events remains unknown. In this study, we investigated GABAA N-methyl-D-aspartate (NMDA) activity control slices regulation...