A5101739451- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Phytase and its Applications
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Soil Carbon and Nitrogen Dynamics
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Nitric Oxide and Endothelin Effects
- Algal biology and biofuel production
- RNA Research and Splicing
- Immune cells in cancer
- Cell Image Analysis Techniques
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Enzyme Production and Characterization
- Genetic Associations and Epidemiology
- Agricultural Science and Fertilization
- Metabolomics and Mass Spectrometry Studies
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Plant Micronutrient Interactions and Effects
Tsinghua University
2023-2025
Shanghai Jiao Tong University
2022-2025
Xinjiang Agricultural University
2024-2025
Shanghai Center for Brain Science and Brain-Inspired Technology
2023-2025
Shanghai Mental Health Center
2023-2025
Weifang University
2025
Yale University
2025
Liaocheng People's Hospital
2015-2024
Jiangnan University
2024
Zhejiang Provincial People's Hospital
2024
Here we reported a novel ultrasound-induced polymerization to achieve polyacrylamide–sodium alginate (SA) dual network hydrogel via redox reaction with MXene (Ti 3 C 2 ) as reductant and potassium persulfate (KPS) an oxidant.
Aerobic glycolysis is essential for tumor growth and survival. Activation of multiple carcinogenic signals contributes to metabolism reprogramming during malignant transformation cancer. Recently nitric oxide has been noted promote but the mechanism remains elusive. We report here dual role in glycolysis: low/physiological (≤ 100 nM) promotes ATP production, oxidative defense cell proliferation ovary cancer cells, whereas excess (≥ 500 inhibits it. Nitric a positive effect on by inducing...
The dysfunction of type I interferon (IFN) signaling is an important mechanism immune escape and metastasis in tumors. Increased NOS1 expression has been detected melanoma, which correlated with dysfunctional IFN poor response to immunotherapy, but the specific not determined. In this study, we investigated regulation on clarified relevant molecular mechanisms.After stable transfection A375 cells plasmids, transcription IFNα-stimulated genes (ISGs) were assessed using pISRE luciferase...
This study was conducted to obtain the optimal fermentation process improve nutritional value of bean dregs (BD) and soybean meal (SBM) using single-factor experiments response surface methodology with small peptide content as index. The follows: Bacillus subtilis selected for aerobic 12 h, Saccharomyces cerevisiae + Lactobacillus plantarum rhamnosus anaerobic fermentation, ratio BD SBM = 1:1, 0.65% acid protease +0.65% papain, 15% wheat middlings, 5% inoculum size, 34 °C temperature, 11 d...
Oxygen-independent, flavin mononucleotide-based fluorescent proteins (FbFPs) are promising alternatives to green protein in anaerobic contexts. Deep mutational scanning performs systematic profiling of sequence–function relationships but has not been applied FbFPs. Focusing on CreiLOV from Chlamydomonas reinhardtii, we created and analyzed two comprehensive mutant collections: (1) single-residue, site-saturation mutagenesis libraries covering all 118 residues; (2) a full combinatorial...
One of the malignant transformation hallmarks is metabolism reprogramming, which plays a critical role in biosynthetic needs unchecked proliferation, abrogating cell death programs, and immunologic escape. However, mechanism metabolic switch not fully understood. Here, we found that S-nitrosoproteomic profile endogenous nitrogen oxide ovarian cancer cells targeted multiple components processes. Phosphofructokinase (PFKM), one most important regulatory enzymes glycolysis, was S-nitrosylated...
<title>Abstract</title> Large-scale foundation models have recently opened a new avenue to artificial general intelligence for life sciences, showing great promise in the analysis of single-cell transcriptomic data. Nevertheless, such challenges as tremendous number signaling regions, extreme data sparsity, and nearly binary nature epigenomic prevented construction model epigenomics thus far, though it is evident that abundant properties chromatin accessibility provide more decisive insights...
ABSTRACT Aims The challenges in identifying functional variants from genome‐wide association studies (GWAS) and unraveling regulatory mechanisms schizophrenia research persist, particularly intronic regions. A non‐coding variant, rs1399178, associated with risk, is identified its impact on NRF1 binding investigated. Methods This study focuses GWAS risk loci, using genomics, expression analyses structural analysis to identify 736 single‐nucleotide polymorphisms (SNPs) that disrupt...
The pleiotropic effects of human disease and the complex nature gene-interaction networks require knock-in mice allowing for multiplexed gene perturbations. Here we describe a series with C57BL/6 background conditional or constitutive expression LbCas12a high-fidelity enhanced AsCas12a, which were inserted at Rosa26 locus. Cas12a in did not lead to discernible pathology enabled efficient genome engineering. We used retrovirus-based immune-cell engineering CD4+ CD8+ T cells, B cells...
Appropriate calcium treatments help maintain the appearance, nutritional quality, and postharvest quality of apples, reducing losses during storage. This study investigated effects different preparations on fresh-cut ultrastructure ‘Starkrimson’ apples. The included control (CK), chloride (T1), sorbitol-chelated (T2), nitrate (T3). results demonstrated that significantly inhibited decline in firmness pectin content while increase cellulose minimizing ultrastructural damage. Apples treated...
Tomato cultivation is seriously affected by infection from
Infantile spasms syndrome (ISS) is an early-onset epileptic encephalopathy characterized by uncontrollable seizures, severe electroencephalogram abnormalities, as well delayed cognitive and behavioral development. Independent studies have shown that a variety of genes are involved in ISS genetic factors play critical role its pathogenesis. Here we report two de novo mutations the casein kinase 1 isoform alpha (CSNK1A1) gene which underlie epilepsy with similar clinical presentation patients....
Numerous studies have reported the importance of soil organic carbon (SOC) in grassland ecosystems and its response to degradation, but effect biochar application on SOC pools degraded alpine grasslands remains unclear. Here, we set up nine addition treatments with a combination three different particle sizes (0~0.25 mm, 0.25~1 1~2 mm) additions (1%, 2%, 4%), conducted short-term observational experiment over 7-month period non-degraded (ND), lightly (LD), severely (SD) zones grassland. The...
Abstract DDX3X neurodevelopmental disorder (DDX3X-NDD) represents a recently identified genetic syndrome characterized by intellectual disability (ID) and developmental delays, primarily caused pathogenic variants in the gene. The physiological ramifications of these mutations remain largely unexplored. In this study, we reported 21 from 22 Chinese patients with DDX3X-NDD whole exome sequencing. We selected five for further functional analyses, including two previously our group. Three...
Abstract Tempol (4-hydroxy-2,2,6,6-Tetramethylpiperidine-1-oxyl, TPL), a nitroxide compound, inhibits proliferation and increases the vulnerability of cancer cells to apoptosis induced by cytotoxic agents. However, molecular mechanism TPL inhibiting cell has not been fully understood. In this study, we evaluated metabolic effect on explored its therapeutic potential. Extracellular flow assays showed that inhibited cellular basal maximal oxygen consumption rates mitochondrial. 13 C flux...
Zinc-finger protein 750 (ZNF750) encodes a putative C2H2 zinc finger and is typically mutated or deleted in squamous cell carcinoma. The role of ZNF750 oral carcinoma (OSCC) remains unknown. aim the present study was to investigate effects overexpression CAL-27 cells. Cell viability, expression genes associated with proliferation, differentiation epithelial-mesenchymal transition were investigated cells following overexpression, using Counting kit-8, reverse transcription-quantitative...
Lanthipeptides are a major class of ribosomally synthesized and post-translationally modified peptides (RiPPs) characterized by thioether cross-links called lanthionine (Lan) methyllanthionine (MeLan). Previously, we developed method to produce mature lanthipeptides in recombinant Escherichia coli, but manual steps hinder large-scale analogue screening. Here devised an automated workflow for creating screening variant libraries haloduracin, two-component II lanthipeptide. An integrated work...