A5100323564- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Nicotinic Acetylcholine Receptors Study
- Pluripotent Stem Cells Research
- Neurological Disease Mechanisms and Treatments
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Robotic Path Planning Algorithms
- Virus-based gene therapy research
- Photonic Crystal and Fiber Optics
- Plant tissue culture and regeneration
- Optical Network Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Neurological Disorders and Treatments
- Arctic and Antarctic ice dynamics
- Neuroscience and Neuropharmacology Research
- Transgenic Plants and Applications
- Advanced Fiber Laser Technologies
- Photosynthetic Processes and Mechanisms
- Ion channel regulation and function
- Microbial Metabolic Engineering and Bioproduction
Changsha Medical University
2020-2025
Central South University
2025
Central South University of Forestry and Technology
2025
Hefei University of Technology
2024
Chinese Academy of Sciences
2013-2024
Shanghai Normal University
2020-2024
Institute of Geochemistry
2024
Shanghai University of Traditional Chinese Medicine
2024
National University of Defense Technology
2002-2023
Hebei University of Chinese Medicine
2023
Genome editing is a valuable technique for gene function analysis and crop improvement. Over the past two years, CRISPR-Cas9 system has emerged as powerful tool precisely targeted editing. In this study, we predicted 11 U6 genes in soybean (Glycine max L.). We then constructed vectors (pCas9-GmU6-sgRNA pCas9-AtU6-sgRNA) using U6-10 Arabidopsis U6-26 promoters, respectively, to produce synthetic guide RNAs (sgRNAs) mutagenesis. Three genes, Glyma06g14180, Glyma08g02290 Glyma12g37050, were...
Summary The Streptococcus ‐derived CRISPR /Cas9 system is being widely used to perform targeted gene modifications in plants. This customized endonuclease has two components, the single‐guide RNA (sg ) for target DNA recognition and ‐associated protein 9 (Cas9) cleavage. Ubiquitously expressed systems ( UC generate with high efficiency but only those produced reproductive cells are transmitted next generation. We report design characterization of a germ‐line‐specific Cas9 GSC Arabidopsis...
Currently, the recovery of cognitive function has become an essential part stroke rehabilitation. DL-3-n-butylphthalide (NBP) is a neuroprotective reagent and been used in treatment. Clinical studies have confirmed that NBP can achieve better outcomes ischemic patients than healthy controls. In this study, we aimed to investigate influences on reperfusion (I/R) rat model. Our results showed profoundly decreased neurological scores, reduced cerebral infarct areas enhanced blood flow (CBF)....
Genome editing technologies are powerful tools for studying gene function and crop improvement. The rely on engineered endonucleases to generate double stranded breaks (DSBs) at target loci. DSBs repaired through the error-prone non-homologous end joining (NHEJ) homology-directed repair (HDR) pathways in cells, resulting mutations sequence replacement, respectively. In widely used CRISPR/Cas9 system, endonuclease Cas9 is targeted by a CRISPR small RNA DNA of interest. this review, we...
CRISPR/Cas9-mediated genome editing is a powerful technology that has been used for the genetic modification of number crop species. In order to evaluate efficacy CRISPR/Cas9 in root crop, sweet potato (Ipomoea batatas), two starch biosynthetic pathway genes, IbGBSSI (encoding granule-bound synthase I), and IbSBEII branching enzyme II), were targeted starch-type cultivar Xushu22 carotenoid-rich Taizhong6. I. batatas was transformed using binary vector, which Cas9 gene driven by Arabidopsis...
Base editor technology that enables precise base-editing has been developed by employing Cas9 nickase (nCas9) or deactivated (dCas9) fused to an enzyme with base conversion activity, named cytidine-deaminase-mediated (CBE, C•G T•A) adenine-deaminase-mediated (ABE, A•T G•C) (Gaudelli et al., 2017; Komor 2016). The systems have successfully applied in several plant species, including Arabidopsis, rice, wheat, maize, tomato and cotton (Mao 2019). However, it is not clear whether editing will...
Summary CRISPR /Cas9 and TALEN are currently the two systems of choice for genome editing. We have studied efficiency system in rice as well nature inheritability ‐induced mutations found important features this technology. The N287C230 backbone resulted low mutation rates (0–6.6%), but truncations its C‐terminal domain dramatically increased to 25%. In most transgenic T0 plants, produced a single prevalent accompanied by variety low‐frequency mutations. For each independent plant, was...
A new deaminase, TadA8e, was recently evolved in the laboratory. TadA8e catalyzes DNA deamination over 1,000 times faster than ABE7.10. We developed a high-efficiency adenine base editor, rABE8e (rice ABE8e), combining monomeric bis-bpNLS and codon optimization. had substantially increased editing efficiencies at NG-protospacer adjacent motif (PAM) NGG-PAM target sequences compared with ABEmax. For most targets, exhibited nearly 100% efficiency high homozygous substitution rates specific...
The present study evaluated the effect of Scriptaid, a novel histone deacetylase inhibitor (HDACi), on in vitro development somatic cell nuclear transfer (SCNT) bovine embryos. Average fluorescence intensity two epigenetic markers (H3K9ac and H3K9m2) at two-cell, eight-cell, blastocyst stages, expression levels developmental important genes (Oct4 IFN-t) stage were also examined to assess influence Scriptaid reprogramming SCNT results showed that treatment with 500 nM for 14 h after...
In vertebrates, the majority of mRNAs that encode secreted, membrane-bound or mitochondrial proteins contain RNA elements activate an alternative mRNA nuclear export (ALREX) pathway. Here we demonstrate containing ALREX-promoting are trafficked through speckles. Although enhance speckle localization, additional features within largely drive this process. Depletion two TREX-associated helicases, UAP56 and its paralog URH49, inhibition transport factor, TAP, not only inhibits ALREX, but also...
Mutations in RanBP2 (also known as Nup358), one of the main components cytoplasmic filaments nuclear pore complex, contribute to overproduction acute necrotizing encephalopathy (ANE1)-associated cytokines. Here we report that represses translation interleukin 6 (IL6) mRNA, which encodes a cytokine is aberrantly up-regulated ANE1. Our data indicates soon after its production, IL6 messenger ribonucleoprotein (mRNP) recruits Argonautes bound let-7 microRNA. After this mRNP exported cytosol,...
In light of the detrimental effects global warming, exacerbated by intense sunlight and high temperatures prevalent during summer in southern China, on growth Melaleuca alternifolia (M. alternifolia) seedlings, this study sets out to examine impact various shading treatments physiological attributes M. with aim determining optimum level. The seedling phase is paramount importance cycle regeneration process alternifolia, as both excessive insufficient exposure can negatively influence...