A5031974129- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Lipid metabolism and biosynthesis
- Plant nutrient uptake and metabolism
- Pharmacological Effects of Natural Compounds
- Biofuel production and bioconversion
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
- Ginseng Biological Effects and Applications
- Enzyme Catalysis and Immobilization
- Molecular Biology Techniques and Applications
- Fungal Infections and Studies
- Plant Virus Research Studies
- Biochemical and Molecular Research
- Light effects on plants
- Natural product bioactivities and synthesis
- Pancreatitis Pathology and Treatment
- Plant Parasitism and Resistance
- CRISPR and Genetic Engineering
University of Kentucky
2016-2025
Nanjing Medical University
2023-2025
South China Botanical Garden
2017-2023
Chinese Academy of Sciences
2017-2023
General Hospital of Central Theater Command
2023
Shanghai Traditional Chinese Medicine Hospital
2023
Huazhong Agricultural University
2022
University of Chinese Academy of Sciences
2021
First People's Hospital of Jingzhou
2021
Sichuan University
2017-2020
Abstract Catharanthus roseus produces a large array of terpenoid indole alkaloids (TIAs) that are an important source natural or semisynthetic anticancer drugs. The biosynthesis TIAs is tissue specific and induced by certain phytohormones fungal elicitors, indicating the involvement complex transcriptional control network. However, regulation TIA pathway poorly understood. Here, we describe C. WRKY transcription factor, CrWRKY1, preferentially expressed in roots jasmonate, gibberellic acid,...
Catharanthus roseus produces bioactive terpenoid indole alkaloids (TIAs), including the chemotherapeutics, vincristine and vinblastine. Transcriptional regulation of TIA biosynthesis is not fully understood. The jasmonic acid (JA)-responsive AP2/ERF transcription factor (TF), ORCA3, its regulator, CrMYC2, play key roles in biosynthesis. ORCA3 forms a physical cluster with two uncharacterized AP2/ERFs, ORCA4 5. Here, we report that (1) ORCA gene differentially regulated; (2) ORCA4, while...
CRISPR/Cas9-mediated genome editing is a powerful technology that has been used for the genetic modification of number crop species. In order to evaluate efficacy CRISPR/Cas9 in root crop, sweet potato (Ipomoea batatas), two starch biosynthetic pathway genes, IbGBSSI (encoding granule-bound synthase I), and IbSBEII branching enzyme II), were targeted starch-type cultivar Xushu22 carotenoid-rich Taizhong6. I. batatas was transformed using binary vector, which Cas9 gene driven by Arabidopsis...
To combat infection to biotic stress plants elicit the biosynthesis of numerous natural products, many which are valuable pharmaceutical compounds. Jasmonate is a central regulator defense response pathogens and accumulation specialized metabolites. Catharanthus roseus produces large number terpenoid indole alkaloids (TIAs) an excellent model for understanding regulation this class Recent work illustrates possible role WRKY transcription factors (TFs) in regulating TIA biosynthesis. In...
Flavonols as plant secondary metabolites with vital roles in development and defense against UV light, have been demonstrated to be the main bioactive components genus Epimedium plants, several species of which are used materials for Herba Epimedii, an important traditional Chinese medicine. The flavonol biosynthetic pathway genes had already isolated from E. sagittatum, but a R2R3-MYB transcription factor regulating synthesis has not functionally characterized so far plants. In this study,...
Catharanthus roseus is the exclusive source of an array terpenoid indole alkaloids including anticancer drugs vincristine and vinblastine, derived from coupling catharanthine vindoline. Leaf-synthesized vindoline regulated by light. A seven-step enzymatic process involved in sequential conversion tabersonine to vindoline; however, regulatory mechanism controlling expression genes encoding these enzymes has not been elucidated. Here, we identified CrGATA1, Leu-Leu-Met domain GATA...
We searched for novel imprinted genes in a region of human chromosome 11p15.5, which contains several known genes. Here we describe the cloning and characterization IPL ( I mprinted P lacenta L iver) gene, shows tissue-specific expression functional imprinting, with maternal allele active paternal relatively inactive, many mouse tissues. Human is highly expressed placenta low but detectable fetal adult liver lung. Mouse Ipl maps to 7 syntenic 11p15.5 this gene at higher levels extraembryonic...
Two cDNA clones with homology to known desaturase genes were isolated from the fungus Mortierella alpina. The open reading frame in one clone encoded 399 amino acids and exhibited delta12-desaturase activity when expressed Saccharomyces cerevisiae presence of endogenous fatty acid substrate oleic acid. insert another contained an encoding 457 delta6-desaturase S. exogenous linoleic Expression gene under appropriate media temperature conditions led production at levels up 25% total yeast....
The maize R2R3-MYB regulator C1 cooperates with the basic helix–loop–helix (bHLH) factor R to activate expression of anthocyanin biosynthetic genes coordinately. As is case for other bHLH factors, harbors several protein–protein interaction domains. Here we show that not classical but rather a briefly extended region forms homodimers bind canonical G-box DNA motifs. This DNA-binding activity abolished if C-terminal ACT (aspartokinase, chorismate, and TyrA) domain licensed homodimerize. Then...
Ubiquitin/26S proteasome (UPS)-dependent proteolysis of a variety cellular proteins plays an essential role in many basic processes. UPS impacts transcriptional regulation by controlling the stability, and thus activity, numerous transcription factors (TFs). In Arabidopsis, trichome development flavonoid metabolism are intimately connected, several TFs have been identified that simultaneously control both Here we show UPS-dependent two these TFs, GLABROUS 3 (GL3) ENHANCER OF GL3 (EGL3), is...
Summary The pharmaceutically valuable monoterpene indole alkaloids ( MIA s) in Catharanthus roseus are derived from the and iridoid pathways that respond to jasmonate JA ) signaling. Two classes of ‐responsive bHLH transcription factor TF ), Cr MYC 2 BIS 1/ 2, known regulate pathways, respectively. However, upregulation either one genes does not lead increased accumulation. Moreover, little is about interconnection between transcriptional cascades hierarchical position Here, we report a...
Herba epimedii (Epimedium), a traditional Chinese medicine, has been widely used as kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba are mainly prenylated flavonol glycosides, end-products the flavonoid pathway. Epimedium species also garden plants due to colorful flowers leaves. Many R2R3-MYB transcription factors (TFs) have identified regulate anthocyanin biosynthetic pathways. However, little is known about TFs involved regulation pathway...