A5066652500- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Innovation and Socioeconomic Development
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- GABA and Rice Research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Seed and Plant Biochemistry
- Seed Germination and Physiology
- RNA regulation and disease
- Plant Reproductive Biology
- Soybean genetics and cultivation
- Photosynthetic Processes and Mechanisms
Chinese Academy of Sciences
2013-2021
Center for Excellence in Molecular Plant Sciences
2013-2021
University of Chinese Academy of Sciences
2013-2018
Shanghai Institutes for Biological Sciences
2013-2017
The CRISPR/Cas9 system has been demonstrated to efficiently induce targeted gene editing in a variety of organisms including plants. Recent work showed that CRISPR/Cas9-induced mutations Arabidopsis were mostly somatic the early generation, although some could be stably inherited later generations. However, it remains unclear whether this will similarly crops such as rice. In study, we tested two rice subspecies 11 target genes for their amenability and determined patterns, specificity...
Significance The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been used to generate targeted gene editing in plants. However, it is not known whether CRISPR/Cas-induced modifications plants occur somatic cells only or some all of the can enter germ line become heritable. Through systematic and multigenerational analysis, this study demonstrates that although majority detected first generation CRISPR/Cas transgenic Arabidopsis were...
Significance Seed dormancy is a critical step in the lifecycle of plants, and it crucial to survival plant species; this process also important for agricultural practice prevent preharvest sprouting when humid conditions persist before harvest. This study uncovers previously unrecognized action auxin maintaining seed through AUXIN RESPONSE FACTOR 10/16-mediated expression ABSCISIC ACID INSENSITIVE 3 , key regulator abscisic acid-mediated dormancy.
Abstract Homologous recombination-based gene targeting is a powerful tool for precise genome modification and has been widely used in organisms ranging from yeast to higher such as Drosophila mouse. However, plants, including the most model plant Arabidopsis thaliana , remains challenging. Here we report sequential transformation method . We find that parental lines expressing bacterial endonuclease Cas9 egg cell- early embryo-specific DD45 promoter can improve frequency of single-guide...
RNA interference (RNAi) in plants can move from cell to cell, allowing for systemic spread of an antiviral immune response. How this cell-to-cell silencing is regulated currently unknown. Here, we describe that the C4 protein Tomato yellow leaf curl virus inhibit intercellular RNAi. Using viral as a probe, have identified receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1) positive regulator movement RNAi, and determined BAM1 its closest homolog, BAM2, play redundant role process....
Summary The Streptococcus ‐derived CRISPR /Cas9 system is being widely used to perform targeted gene modifications in plants. This customized endonuclease has two components, the single‐guide RNA (sg ) for target DNA recognition and ‐associated protein 9 (Cas9) cleavage. Ubiquitously expressed systems ( UC generate with high efficiency but only those produced reproductive cells are transmitted next generation. We report design characterization of a germ‐line‐specific Cas9 GSC Arabidopsis...
Plants flower in response to many varied cues, such as temperature, photoperiod, and age. The floral transition of Cardamine flexuosa, a herbaceous biennial-to-perennial plant, requires exposure cold treatment known vernalization. C. flexuosa younger than 5 weeks old are not fully responsive treatment. We demonstrate that the levels two age-regulated microRNAs, miR156 miR172, regulate timing sensitivity Age vernalization pathways coordinately flowering through modulating expression CfSOC1,...
The CRISPR/Cas9 system has been widely used for targeted genome editing in numerous plant species. In Arabidopsis, constitutive promoters usually result a low efficiency of heritable mutation the T1 generation. this work, gene efficiencies using different to drive Cas9 expression were evaluated. Expression under CaMV 35S promoter resulted 2.3% rate plants and failed produce homozygous mutations T2 generations. contrast, two cell division-specific promoters, YAO CDC45, produced rates 80.9%...
It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair Arabidposis. However, important questions remain regarding biogenesis of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs Arabidopsis rice. We found 21-nt were from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9. Unexpectedly, Pol II transcription was required for efficient...
DNA cytosine methylation confers stable epigenetic silencing in plants and many animals. However, the mechanisms underlying methylation-mediated genomic are not fully understood. We conducted a forward genetic screen for cellular factors required of heavily methylated p35S:NPTII transgene Arabidopsis thaliana rdm1-1 mutant background, which led to identification Hsp20 family protein, RDS1 (rdm1-1 suppressor 1). Loss-of-function mutations released p35S::NPTII plants, without changing state...
To clone and characterize the DNA cDNA sequences of phenylalanine ammonia-lyase gene (PAL) from Fagopyrum dibotrys, investigate biological activity obtained PAL.Using homology cloning RT-PCR techniques, full-length PAL were amplified F. dibotrys. The analyzed by bioinformatics software. ORF was cloned into expression vector pET-30b(+) transformed Escherichia coli BL21 (DE3) for recombined protein. catalytic protein determined Spectrophotometer thin layer chromatography (TLC) methods.The...
ABSTRACT RNA interference (RNAi) in plants can move from cell to cell, allowing for systemic spread of an anti-viral immune response. How this cell-to-cell silencing is regulated currently unknown. Here, we describe that the C4 protein Tomato yellow leaf curl virus has ability inhibit intercellular RNAi. Using viral as a probe, have identified receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1) positive regulator movement RNAi, and determined BAM1 its closest homologue, BAM2, play...