A5051930466- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Insect symbiosis and bacterial influences
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Organometallic Complex Synthesis and Catalysis
- Animal Genetics and Reproduction
- Asymmetric Hydrogenation and Catalysis
- Carbon dioxide utilization in catalysis
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Mapping and Diversity in Plants and Animals
- Virus-based gene therapy research
- Plant Physiology and Cultivation Studies
- Innovation and Socioeconomic Development
- Banana Cultivation and Research
- GABA and Rice Research
- Cytomegalovirus and herpesvirus research
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Agricultural Practices and Plant Genetics
- Seed Germination and Physiology
Shanghai Chenshan Plant Science Research Center
2024
Center for Excellence in Molecular Plant Sciences
2017-2021
Chinese Academy of Sciences
2014-2021
University of Chinese Academy of Sciences
2014-2021
State Key Laboratory of Plant Genomics
2014-2021
Institute of Genetics and Developmental Biology
2014-2021
Shanghai Advanced Research Institute
2020
Abscisic acid (ABA) is a key phytohormone that controls plant growth and stress responses. It sensed by the pyrabactin resistance 1 (PYR1)/PYR1-like (PYL)/regulatory components of ABA receptor (RCAR) family proteins. Here, we utilized CRISPR/Cas9 technology to edit group I (PYL1-PYL6 PYL12) II (PYL7-PYL11 PYL13) PYL genes in rice. Characterization combinatorial mutants suggested have more important roles stomatal movement, seed dormancy, regulation than those II. Among all single pyl...
Summary Base editing is a novel genome strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine editors with engineered SpCas9 SaCas9 variants substantially expand targetable sites in rice genome. These can edit endogenous genes various efficiencies. Moreover, show be simultaneously executed rice. The described here will useful functional genomics research...
Dear editor, Making precise changes in the genomes of organisms is challenging for most genome editing tools. Recently, a search-and-replace method, also known as prime editing, was developed that can introduce user-defined sequence into target site without requiring double-stranded breaks (DSBs) or repair templates (Anzalone et al., 2019). The editor contains Moloney murine leukaemia virus reverse transcriptase (M-MLV RT) fused to C terminus SpCas9 (H840A) nickase This fusion protein guided...
Abstract The widespread agricultural problem of pre-harvest sprouting (PHS) could potentially be overcome by improving seed dormancy. Here, we report that miR156, an important grain yield regulator, also controls dormancy in rice. We found mutations one MIR156 subfamily enhance and suppress PHS with negligible effects on shoot architecture size, whereas another modify increase size but have minimal Mechanistically, mir156 suppressing the gibberellin (GA) pathway through de-represssion miR156...
Summary Adenine base editors ( ABE s) have been exploited to introduce targeted adenine (A) guanine (G) conversions in various plant genomes, including rice, wheat and Arabidopsis . However, the s reported thus far are all quite inefficient at many target sites which hampers their applications genome engineering crop breeding. Here, we show that unlike mammalian system, a simplified editor ‐P1S (Adenine Base Editor‐Plant version 1 Simplified) containing ecTadA*7.10‐ nSpC as9 (D10A) fusion...
Panicle size and grain number are important agronomic traits influence yield in rice (Oryza sativa), but the molecular genetic mechanisms underlying panicle control remain largely unknown crops. Here we report that LARGE2 encodes a HECT-domain E3 ubiquitin ligase OsUPL2 regulates rice. The loss of function large2 mutants produce large panicles with increased number, wide grains leaves, thick culms. by repressing meristematic activity. is highly expressed young grains. Biochemical analyses...
Alkaline salt stress adversely affects rice growth, productivity and grain quality. However, the mechanism underlying this process remains elusive. We characterized here an alkaline tolerant mutant, alt1 in rice. Map-based cloning revealed that harbors a mutation chromatin remodeling ATPase gene. ALT1-RNAi transgenic plants under different genetic background mimicked phenotype, exhibiting tolerance to transcript dosage-dependent manner. The predicted ALT1 protein belonged Ris1 subgroup of...
The CRISPR/Cas9 system has been widely used for targeted genome editing in numerous plant species. In Arabidopsis, constitutive promoters usually result a low efficiency of heritable mutation the T1 generation. this work, gene efficiencies using different to drive Cas9 expression were evaluated. Expression under CaMV 35S promoter resulted 2.3% rate plants and failed produce homozygous mutations T2 generations. contrast, two cell division-specific promoters, YAO CDC45, produced rates 80.9%...
Abstract Combining CO 2 and H to prepare building blocks for high-value-added products is an attractive yet challenging approach. A general selective rhodium-catalyzed hydroformylation of alkenes using /H as a syngas surrogate described here. With this protocol, the desired aldehydes can be obtained in up 97% yield 93/7 regioselectivity under mild reaction conditions (25 bar, 80 ºC). Key-to-success use bifunctional Rh/PTA catalyst (PTA: 1,3,5-triaza-7-phosphaadamantane), which facilitates...