A5029928792- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Polysaccharides and Plant Cell Walls
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Plant Micronutrient Interactions and Effects
- Plant Gene Expression Analysis
- Ubiquitin and proteasome pathways
- Plant responses to water stress
- Seed Germination and Physiology
- Magnetic and Electromagnetic Effects
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Studies on Chitinases and Chitosanases
- Light effects on plants
- Plant Surface Properties and Treatments
- Insect Pest Control Strategies
Purdue University West Lafayette
2012-2022
King Abdulaziz University
2012-2015
King Abdullah University of Science and Technology
2009-2014
Gyeongsang National University
2004-2014
University of Illinois Urbana-Champaign
2004-2012
Purdue University System
2011-2012
U.S. Arid Land Agricultural Research Center
2011
United States Department of Agriculture
2011
Agricultural Research Service
2011
University of Arizona
2001-2010
Plant responses to salinity stress are reviewed with emphasis on molecular mechanisms of signal transduction and the physiological consequences altered gene expression that affect biochemical reactions downstream sensing. We make extensive use comparisons model organisms, halophytic plants, yeast, which provide a paradigm for many exhibited by stress-sensitive plants. Among responses, we emphasize osmolyte biosynthesis function, water flux control, membrane transport ions maintenance...
Homeostasis can be defined as the tendency of a cell or an organism to maintain internal steady state, even in response any environmental perturbation stimulus tending disturb normality, because coordinate responses its constituent components. Typically, ions constantly flux and out cells controlled fashion with net adjusted accommodate cellular requirements, thus creating ionic homeostasis. When plant are exposed salinity, mediated by high NaCl concentrations, kinetic states ion transport...
Abstract SIZ1 is a SUMO E3 ligase that facilitates conjugation of to protein substrates. siz1-2 and siz1-3 T-DNA insertion alleles caused freezing chilling sensitivities were complemented genetically by expressing SIZ1, indicating the controller low temperature adaptation in plants. Cold-induced expression CBF/DREB1, particularly CBF3/DREB1A, regulon genes was repressed siz1. siz1 did not affect ICE1, which encodes MYC transcription factor CBF3/DREB1A. A K393R substitution ICE1 [ICE1(K393R)]...
Plants sense phosphate (Pi) deficiency and initiate signaling that controls adaptive responses necessary for Pi acquisition. Herein, evidence establishes AtSIZ1 is a plant small ubiquitin-like modifier (SUMO) E3 ligase focal controller of starvation-dependent responses. T-DNA insertional mutated alleles (At5g60410) cause Arabidopsis to exhibit exaggerated prototypical starvation responses, including cessation primary root growth, extensive lateral hair development, increase in root/shoot...
Significance We identified transgenic plants that are extremely resistant to drought from a large-scale screening of overexpressing the pyrabactin resistance 1-like (PYL) family abscisic acid (ABA) receptors. explored how these resist by examining both short-term responses, such as stomatal closure, and long-term senescence. The physiological roles ABA-induced senescence under stress conditions underlying molecular mechanism unclear. Here, we found ABA induces activating ABA-responsive...
Summary The Arabidopsis thaliana vacuolar Na + /H antiporter AtNHX1 is a salt tolerance determinant. Predicted amino acid sequence similarity, protein topology and the presence of functional domains conserved in prototypical mammalian NHE exchangers led to identification five additional AtNHX genes (AtNHX2–6). AtNHX2 mRNAs are most prevalent transcripts among this family seedling shoots roots. A lower‐abundance AtNHX5 mRNA present both roots, whereas AtNHX3 transcript expressed predominantly...
Combinations of ethylene and methyl jasmonate (E/MeJA) synergistically induced members both groups 1 5 the pathogenesis-related (PR) superfamily defense genes. E/MeJA caused a synergistic induction PR-1b osmotin (PR-5) mRNA accumulation in tobacco seedlings. also activated promoter fused to [beta]-glucuronidase marker gene tissue-specific manner. The responsiveness was localized on -248 +45 fragment that exhibited several other inducers. resulted protein levels similar those by osmotic...
Two Arabidopsis thaliana extragenic mutations that suppress NaCl hypersensitivity of the sos3-1 mutant were identified in a screen T-DNA insertion population genetic background Col-0 gl1 sos3-1. Analysis genome sequence region flanking left border indicated hkt1-1 and hkt1-2 plants have allelic AtHKT1. AtHKT1 mRNA is more abundant roots than shoots wild-type but not detected either mutant, indicating this gene inactivated by mutations. can to an equivalent extent Na(+) sensitivity seedlings...
Flowering of the reference plant Arabidopsis thaliana is controlled by several signaling pathways, which converge on a small set genes that function as pathway integrators. We have analyzed genomic response to one type floral inductive signal, photoperiod, dissect transducing this stimulus, including CONSTANS, thought be major output photoperiod pathway. Comparing effects CONSTANS with those FLOWERING LOCUS T, integrates inputs from and other we find expression profiles shoot apices plants...
Cultured tobacco (Nicotiana tabacum var Wisconsin 38) cells adapted to grow under osmotic stress synthesize and accumulate a 26 kilodalton protein (osmotin) which can constitute as much 12% of total cellular protein. In NaCl, osmotin occurs in two forms: an aqueous soluble form (osmotin-I) detergent (osmotin II) the approximate ratio 2:3. Osmotin-I has been purified electrophoretic homogeneity, osmotin-II 90% homogeneity. The N-terminal amino acid sequences osmotins I II are identical...
Summary An interesting observation, reported for transgenic plants that have been engineered to overproduce osmolytes, is they often exhibit impaired growth in the absence of stress. As reduction and accumulation osmolytes both typically result from adaptation, we hypothesized may actually osmolyte accumulation. To examine this possibility more closely, intracellular proline level was manipulated by expressing mutated derivatives tomPRO2 (a Δ 1 ‐pyrroline‐5‐carboxylate synthetase, P5CS,...
Abstract Salt cress (Thellungiella halophila) is a small winter annual crucifer with short life cycle. It has genome (about 2 × Arabidopsis) high sequence identity (average 92%) Arabidopsis, and can be genetically transformed by the simple floral dip procedure. capable of copious seed production. an extremophile native to harsh environments reproduce after exposure extreme salinity (500 mm NaCl) or cold −15°C. typical halophyte that accumulates NaCl at controlled rates also dramatic levels...
Transgenic potato and tobacco plants carrying the osmotin gene under control of cauliflower mosaic virus 35S promoter constitutively overexpressed to a level approximately 2% total cellular protein. Leaves transgenic exhibited delayed development disease symptoms after inoculation with spore suspensions Phytophthora infestans, which is cause late blight potato. In contrast, did not display any change in when challenged either or fungal mycelia parasitica var. nicotianae. Using vitro assays,...
Abstract Plant molecular responses to osmotic stress are complex as evidenced by the isolation of numerous OR (osmotic stress-regulated) genes. Although functions including osmolyte biosynthesis, membrane transport, signal transduction, and cellular protection have been predicted for genes, few them established. Current efforts toward isolating analyzing expression individual genes should be replaced systematic approaches analyze all simultaneously in selected plant species. Both...
Abscisic acid (ABA) is a key phytohormone that controls plant growth and stress responses. It sensed by the pyrabactin resistance 1 (PYR1)/PYR1-like (PYL)/regulatory components of ABA receptor (RCAR) family proteins. Here, we utilized CRISPR/Cas9 technology to edit group I (PYL1-PYL6 PYL12) II (PYL7-PYL11 PYL13) PYL genes in rice. Characterization combinatorial mutants suggested have more important roles stomatal movement, seed dormancy, regulation than those II. Among all single pyl...
Summary Reversible modifications of target proteins by small ubiquitin‐like modifier (SUMO) are involved in many cellular processes yeast and animals. Yet little is known about the function sumoylation plants. Here, we show that SIZ1 gene, which encodes an Arabidopsis SUMO E3 ligase, regulates innate immunity. Mutant siz1 plants exhibit constitutive systemic‐acquired resistance (SAR) characterized elevated accumulation salicylic acid (SA), increased expression pathogenesis‐related ( PR )...
Histone modification in chromatin is one of the key control points gene regulation eukaryotic cells. Protein complexes composed histone acetyltransferase or deacetylase, WD40 repeat protein, and many other components have been implicated this process. Here, we report identification functional characterization HOS15, a WD40-repeat protein crucial for repression genes associated with abiotic stress tolerance through deacetylation Arabidopsis . HOS15 shares high sequence similarity human...
NHX-type antiporters in the tonoplast have been reported to increase salt tolerance of various plants species, and are thought mediate compartmentation Na(+) vacuoles. However, all isoforms characterized so far catalyze both Na(+)/H(+) K(+)/H(+) exchange. Here, we show that AtNHX1 has a critical involvement subcellular partitioning K(+), which turn affects plant K(+) nutrition tolerance. Transgenic tomato overexpressing had larger vacuolar pools growth conditions tested, but no consistent...
Abstract The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, mRNA abundance higher than Arabidopsis. Ectopic expression homolog ThSOS1 suppressed salt-sensitive phenotype Saccharomyces cerevisiae strain lacking sodium ion (Na+) efflux transporters increased...