A5100606026- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Insect symbiosis and bacterial influences
- Bacterial Genetics and Biotechnology
- Microbial bioremediation and biosurfactants
- Vibrio bacteria research studies
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Pharmacological Effects of Natural Compounds
- Innovation and Socioeconomic Development
- Pesticide Residue Analysis and Safety
- Biosensors and Analytical Detection
- Pluripotent Stem Cells Research
- Microbial Community Ecology and Physiology
- Phenothiazines and Benzothiazines Synthesis and Activities
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Nutrition, Genetics, and Disease
- Antimicrobial Peptides and Activities
- Chemical Analysis and Environmental Impact
Chinese Academy of Sciences
2015-2025
Dezhou University
2024-2025
Chongqing Medical University
2023-2025
Children's Hospital of Chongqing Medical University
2023-2025
Institute of Biophysics
2025
Tianjin Institute of Industrial Biotechnology
2025
Xinyang College of Agriculture and Forestry
2025
Zhejiang Normal University
2023-2025
Henan Agricultural University
2025
Peking University
2013-2025
The genomes of five Cochliobolus heterostrophus strains, two sativus three additional species (Cochliobolus victoriae, carbonum, miyabeanus), and closely related Setosphaeria turcica were sequenced at the Joint Genome Institute (JGI). datasets used to identify SNPs between strains species, unique genomic regions, core secondary metabolism genes, small secreted protein (SSP) candidate effector encoding genes with a view towards pinpointing structural elements gene content associated...
Abstract The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system mediates adaptive immunity against foreign nucleic acids in prokaryotes. However, efficient adaptation of a native CRISPR to purified viruses has only been observed for the type II-A from Streptococcus thermophilus industry strain, and rarely reported laboratory strains. Here, we provide second showing adaptation. Infected by newly isolated virus HHPV-2, Haloarcula hispanica I-B acquired spacers...
Summary Previous studies revealed that the promoters for driving both Cas9 and sg RNA s are quite important efficient genome editing by CRISPR /Cas9 in plants. Here, we report our results of targeted using maize dmc1 gene promoter combined with U3 , respectively. Three loci were selected targeting. The T0 plants regenerated highly efficiently edited at target sites homozygous or bi‐allelic mutants accounting about 66%. mutations could be stably transmitted to T1 generation, new generated...
We tested the effects of various putative efflux pump inhibitors on colistin resistance in multidrug-resistant Gram-negative bacteria. Addition 10 mg/liter cyanide 3-chlorophenylhydrazone (CCCP) to test medium could significantly decrease MICs colistin-resistant strains. Time-kill assays showed CCCP reverse and inhibit regrowth resistant subpopulation, especially Acinetobacter baumannii Stenotrophomonas maltophilia These results suggest bacteria can be suppressed reversed by CCCP.
Squalene epoxidase (SQLE), also known as squalene monooxygenase, catalyzes the stereospecific conversion of to 2,3(S)-oxidosqualene, a key step in cholesterol biosynthesis. SQLE inhibition is targeted for treatment hypercholesteremia, cancer, and fungal infections. However, lack structure-function understanding has hindered further progression its inhibitors. We have determined first three-dimensional high-resolution crystal structures human catalytic domain with small molecule inhibitors...
Cyclic GMP-AMP (cGAMP) synthase (cGAS, a.k.a. MB21D1), a cytosolic DNA sensor, catalyzes formation of the second messenger 2'3'-cGAMP that activates stimulator interferon genes (STING) signaling. How cGAS activity is modulated remains largely unknown. Here, we demonstrate sentrin/SUMO-specific protease 7 (SENP7) interacted with and potentiated activation. The small ubiquitin-like modifier (SUMO) was conjugated onto lysine residues 335, 372 382 cGAS, which suppressed its DNA-binding,...
Small RNAs guard CRISPR-Cas The microbial adaptive immunity system benefits microbes by warding off genetic invaders, but it also inflicts a fitness cost because of occasional autoimmune reactions, rendering CRISPR loci evolutionarily unstable. Li et al. identified previously unnoticed toxin-antitoxin RNA pairs embedded within diverse loci. antitoxin mimics and repurposes the effector to transcriptionally repress toxin that would otherwise arrest cell growth sequestering rare transfer RNA....
The resuscitation promoting factor (Rpf) of Micrococcus luteus has been reported to resuscitate and stimulate the growth functional microorganisms that are involved in aerobic degradation polychlorinated biphenyls (PCBs). However, few studies have conducted investigate role Rpf on anaerobic microbial populations.
Abstract CreTA, CRISPR-regulated toxin-antitoxin (TA), safeguards CRISPR-Cas immune systems by inducing cell dormancy/death upon their inactivation. Here, we characterize a bacterial CreTA associating with the I-F in Acinetobacter . CreT is distinct bactericidal small RNA likely targeting several essential molecules that are required to initiate protein synthesis. CreA guides CRISPR effector transcriptionally repress CreT. We further demonstrate proof-of-concept antimicrobial strategy named...
The ability of honey to kill bacterial pathogens in vitro and quickly clear even chronic or drug-resistant infections has been demonstrated by several studies. Most current research is focused on identifying the bactericidal compounds honey, but action discovered not sufficient explain honey's activity. By diluting sub-inhibitory levels, we were able study its impact behavior, that inhibits quorum sensing (QS). Experiments characterize quantify effect QS networks Pseudomonas aeruginosa...
DegP (also designated as HtrA) and its homologs are found in prokaryotic cells such eukaryotic organelles mitochondria chloroplasts. has been to be essential for the growth of Gram-negative bacteria under heat shock conditions arguably considered possess both protease chaperone activities. The function not clearly defined. Using genetically incorporated non-natural amino acids photo-crosslinkers, here we identified β-barrel outer membrane proteins (OMPs) major natural substrates Escherichia...
The treatment of diseases caused by drug-resistant bacterial infections urgently requires new types broad-spectrum antimicrobial materials. Herein, we introduce a supramolecular self-assembly, NanoPcN, which realizes the combination type I photodynamic activity and photothermal effects modifying zinc(II) phthalocyanine with 3-(dimethylamino) phenoxy group. Antibacterial experiments demonstrate that this "one-for-two" property endows NanoPcN excellent efficacy, not only against Gram-positive...
The prokaryotic immune system CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated genes) adapts to foreign invaders by acquiring their short deoxyribonucleic acid (DNA) fragments as spacers, which guide subsequent interference nucleic acids based on sequence matching. adaptation mechanism avoiding 'self' DNA is poorly understood. In Haloarcula hispanica, we previously showed that CRISPR requires being primed a pre-existing spacer partially matching the...
It has been found that the Shuttle Radar Topography Mission (SRTM) digital elevation model (DEM) is systematically higher than actual land surface in vegetated areas. This study developed a new globally corrected SRTM DEM through reducing its systematic bias Over 150, 000 km2 airborne light detection and ranging (LiDAR) data along with spaceborne LiDAR, global canopy height data, cover were collected to correct DEM. A linear regression based method was used estimate original error therefore...
Acinetobacter baumannii is undoubtedly one of the most clinically significant pathogens. The multidrug resistance and virulence potential A. are responsible for hospital-acquired nosocomial infections. Unlike numerous investigations on drug-resistant epidemiology baumanni, molecular less studied. Here, we collected 88 clinical isolates, tested their antimicrobial susceptibility to 10 commonly used antibiotics analyzed distribution 9 selected virulence-associated genes, aims investigate...