A5044740619- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Genetic and phenotypic traits in livestock
- Fish Ecology and Management Studies
- Photosynthetic Processes and Mechanisms
- Reproductive Biology and Fertility
- Mass Spectrometry Techniques and Applications
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Advanced Proteomics Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Epigenetics and DNA Methylation
- Biochemical and Molecular Research
- Polyamine Metabolism and Applications
- Algal biology and biofuel production
- Lipid metabolism and biosynthesis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biochemical and Structural Characterization
- Reproductive biology and impacts on aquatic species
- Protist diversity and phylogeny
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Aquaculture Nutrition and Growth
- Plant Gene Expression Analysis
Donald Danforth Plant Science Center
2014-2024
University of Illinois Urbana-Champaign
2009-2023
University of Sussex
2023
Ernst Strüngmann Institute for Neuroscience
2023
Agriculture and Agri-Food Canada
2022
London Research and Development Centre
2022
Western University
2022
Agricultural Research Service
2022
Vanderbilt University
2022
Vanderbilt Health
2022
Relative to the atmosphere, much of aerobic ocean is supersaturated with methane; however, source this important greenhouse gas remains enigmatic. Catabolism methylphosphonic acid by phosphorus-starved marine microbes, concomitant release methane, has been suggested explain phenomenon, yet methylphosphonate not a known natural product, nor it detected in systems. Further, its synthesis from products would require unknown biochemistry. Here we show that archaeon Nitrosopumilus maritimus...
Significance The discovery of natural products, an important source human medicines, is critical for the development new therapeutics against health threats, including cancer and multidrug-resistant pathogens. Yet, in recent years, industrial pharmaceuticals from products has been stymied due to a variety reasons, repeated previously known compounds. Here, we demonstrate large-scale genomics as one potential solution this problem by mining collection 10,000 actinomycetes novel phosphonic...
Many species possess an endogenous circadian clock to synchronize internal physiology with oscillating external environment. In plants, the coordinates growth, metabolism and development over daily seasonal time scales. proteins in network form complexes that temporally regulate myriad processes, including signal transduction, transcription, protein degradation post-translational modification. Arabidopsis thaliana, a tripartite complex composed of EARLY FLOWERING 4 (ELF4), 3 (ELF3), LUX...
Engineering metabolic biosynthetic pathways has enabled the microbial production of many useful chemicals. However, pathway productivities and yields are often limited by imbalances. Synthetic regulatory circuits have been shown to be able balance engineered pathways, improving titers productivities. Here we developed a negative feedback circuit based on malonyl-CoA-based sensor-actuator. Malonyl-CoA is biosynthesized from acetyl-CoA carboxylase, which rate-limiting step for fatty acid...
Cancer cells are characterized by elevated levels of reactive oxygen species, which produced mainly the mitochondria. The dismutase SOD2 localizes in matrix and is a major antioxidant. activity regulated deacetylase SIRT3. Recent studies indicated that SIRT3 decreased 87% breast cancers, implying compromised. resulting elevation species was shown to be essential for metabolic reprograming toward glycolysis. Here, we show itself down-regulated cancer cell lines. Further, activation oncogenes,...
The networks that govern carbon metabolism and control intracellular partitioning in photosynthetic cells are poorly understood. Target of Rapamycin (TOR) kinase is a conserved growth regulator integrates nutrient signals modulates cell eukaryotes, though the TOR signaling pathway plants algae has yet to be completely elucidated. We screened unicellular green alga Chlamydomonas reinhardtii using insertional mutagenesis find mutants conferred hypersensitivity inhibitor rapamycin....
Plants react to seasonal change in day length through altering physiology and development. Factors that function harmonize growth with photoperiod are poorly understood. Here we characterize a new protein associates both circadian clock photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens independent length. peaks at dusk, binds phytochrome B...
Methods used to quantify protein from biological samples are often inaccurate with significant variability that requires care minimize. The errors result losses during preparation and purification false detection of interfering compounds or elements. Amino acid analysis (AAA) involves a series chromatographic techniques can be measure levels, avoiding some difficulties providing specific compositional information. However, unstable derivatives, toxic costly, incomplete reactions, inadequate...
Synthetic mRNA technology is a promising avenue for treating and preventing disease. Key to the incorporation of modified nucleotides such as N1-methylpseudouridine (m1Ψ) decrease immunogenicity RNA. However, relatively few studies have addressed effects on decoding process. Here, we investigate effect m1Ψ related modification pseudouridine (Ψ) translation. In reconstituted system, find that does not significantly alter accuracy. More importantly, do detect an increase in miscoded peptides...
Summary Steroid alkaloids have been shown to elicit a wide range of pharmacological effects that include anticancer and antifungal activities. Understanding the biosynthesis these molecules is essential bioengineering for sustainable production. Herein, we investigate biosynthetic pathway cyclopamine, steroid alkaloid shows promising antineoplastic Supply cyclopamine limited, as current source solely derived from wild collection plant Veratrum californicum . To elucidate early stages...
Abstract Different intensities of high temperatures affect the growth photosynthetic cells in nature. To elucidate underlying mechanisms, we cultivated unicellular green alga Chlamydomonas reinhardtii under highly controlled photobioreactor conditions and revealed systems-wide shared unique responses to 24-hour moderate (35°C) acute (40°C) subsequent recovery at 25°C. We identified previously overlooked elements response temperature. Heat 35°C transiently arrested cell cycle followed by...
Fosfomycin is a wide-spectrum antibiotic that used clinically to treat acute cystitis in the United States. The compound produced by several strains of streptomycetes and pseudomonads. We sequenced biosynthetic gene cluster responsible for fosfomycin production Pseudomonas syringae PB-5123. Surprisingly, pathway this organism very different from Streptomyces fradiae wedmorensis. pathways share first last steps, involving conversion phosphoenolpyruvate phosphonopyruvate (PnPy)...
Nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) are large enzymes responsible for the biosynthesis of medically ecologically important secondary metabolites. In a previous report, we described proteomics approach to screen expressed NRPSs or PKSs from bacteria with without sequenced genomes. Here used this proteome mining discover novel natural product arising rare adenylation (A) reductase (Red) domains in its biosynthetic machinery. We also cloned entire gene...
Endoplasmic reticulum (ER) stress and disrupted proteostasis contribute to the pathogenesis of a variety glomerular tubular diseases. Thus, it is imperative develop noninvasive biomarkers for detecting ER in podocytes or cells incipient stage disease, when kidney biopsy not yet clinically indicated. Mesencephalic astrocyte–derived neurotrophic factor (MANF) localizes lumen secreted response several cell types. Here, using mouse models human nephrotic syndrome caused by mutant laminin β 2...
Farmed Atlantic salmon (Salmo salar) is a globally important production species, including in Australia where breeding and selection has been progress since the 1960s. The recent development of SNP genotyping platforms means genome-wide association genomic prediction can now be implemented to speed genetic gain. As precursor, this study collected genotypes at 218 132 SNPs 777 fish from Tasmanian population assess levels diversity, strength linkage disequilibrium (LD) imputation accuracy....
Abstract Teleost fish exhibit a remarkable diversity in the control of sex determination, offering opportunity to identify novel differentiation mechanisms and their ecological consequences. Here, we perform GWAS using 4715 46,501 SNP map determination three separate genomic locations Atlantic salmon ( Salmo salar ). To characterize each, whole genome sequencing was performed 30-fold depth coverage 20 representing each identified lineages. polymorphism reveals male carry single copy specific...
Previous studies on capsaicin, the bioactive compound in chili peppers, have shown that it may a beneficial effect vivo when part of regular diet. These positive health benefits, including an anti-inflammatory potential and protective effects against obesity, are often attributed to gut microbial community response capsaicin. However, there is no consensus mechanism behind In this study, we used vitro model human microbiota determine how consumption capsaicin impacts microbiota. Using...
The human pathogen Aspergillus fumigatus makes a series of fumiquinazoline (FQ) peptidyl alkaloids increasing scaffold complexity using L-Trp, 2 equiv L-Ala, and the non-proteinogenic amino acid anthranilate as building blocks. FQ gene cluster encodes two non-ribosomal peptide synthetases (NRPS) flavoproteins. trimodular NRPS Af12080 assembles FQF (the first level complexity) while next enzymes, Af12060 Af12050, act in tandem an oxidative annulation sequence to couple alanine indole side...
A key developmental transformation in the life of all vertebrates is transition to sexual maturity, whereby individuals are capable reproducing for first time. In farming Atlantic salmon, early maturation prior harvest size has serious negative production impacts.We report genome wide association studies (GWAS) using fish measured freshwater or marine environment. Genotypic data from a custom 50 K single nucleotide polymorphism (SNP) array was used identify 13 significantly associated SNP...