A5015076620- Metabolomics and Mass Spectrometry Studies
- Plant nutrient uptake and metabolism
- Lipid metabolism and biosynthesis
- Soybean genetics and cultivation
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Plant biochemistry and biosynthesis
- Metabolism and Genetic Disorders
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Mass Spectrometry Techniques and Applications
- Plant Gene Expression Analysis
- Sphingolipid Metabolism and Signaling
- Seed Germination and Physiology
- Adipose Tissue and Metabolism
- Food composition and properties
- Rice Cultivation and Yield Improvement
- Isotope Analysis in Ecology
- Plant responses to water stress
- Plant Disease Resistance and Genetics
- Phytase and its Applications
- Plant-Microbe Interactions and Immunity
Donald Danforth Plant Science Center
2019-2024
Salk Institute for Biological Studies
2023-2024
Agriculture and Agri-Food Canada
2017-2022
London Research and Development Centre
2017-2022
Agricultural Research Service
2022
Vanderbilt University
2022
Vanderbilt Health
2022
Western University
2017-2022
Trent University
2020-2021
Methods used to quantify protein from biological samples are often inaccurate with significant variability that requires care minimize. The errors result losses during preparation and purification false detection of interfering compounds or elements. Amino acid analysis (AAA) involves a series chromatographic techniques can be measure levels, avoiding some difficulties providing specific compositional information. However, unstable derivatives, toxic costly, incomplete reactions, inadequate...
Protein and oil levels measured at maturity are inversely correlated across soybean lines; however, carbon is in limited supply during maturation resulting tradeoffs for the production of other reserves including oligosaccharides. During late stages seed development, allocation storage changes. Lipid protein decline while concentrations indigestible raffinose family oligosaccharides (RFOs) increase, leading to a decreased crop value. Since maternal source diminished supplied RFO synthesis...
The negative association between protein and oil production in soybean (Glycine max) seed is well-documented. However, this inverse relationship based primarily on the composition of mature seed, which reflects cumulative result events over course development therefore does not convey information specific to metabolic fluctuations during developmental growth regimes. In study, we assessed maternal nutrient supply via measurement coat exudates metabolite levels within cotyledon throughout...
The metabolic plasticity of tobacco leaves has been demonstrated via the generation transgenic plants that can accumulate over 30% dry weight as triacylglycerols. In investigating changes in carbon partitioning these high lipid-producing (HLP) leaves, foliar lipids accumulated stepwise development. Interestingly, non-transient starch was observed to with plant age WT but not HLP a drop concurrent an increase lipid content. tradeoff between and studied using 13CO2-labeling experiments...
In developing soybean seeds, carbon is partitioned between oil, protein and carbohydrates. Here, we demonstrate that suppression of lipase-mediated turnover triacylglycerols (TAG) during late seed development increases fatty acid content decreases the presence undigestible oligosaccharides. During stages embryo development, while levels oligosaccharides raffinose stachyose increase. Three genes orthologous to Arabidopsis lipase gene SUGAR-DEPENDENT1 ( SDP1 ) are upregulated at this time....
Cytokinins (CKs) are adenine derivatives that act as phytohormones. These signaling molecules control plant cell division and differentiation, organ growth, senescence, they orchestrate interactions with biotic abiotic environments. While CKs predominately recognized plant-based substances, have been found across different domains of life, including microorganisms, insects, mammals, humans. In plants, at trace, often low femtomolar concentrations; therefore, sensitive precise analytical...
Abstract Cytokinins (CKs) play a fundamental role in regulating dynamics of organ source/sink relationships during plant development, including flowering and seed formation stages. As result, CKs are key drivers yield. The cytokinin oxidase/dehydrogenase (CKX) is one the critical enzymes responsible for CK levels by causing their irreversible degradation. Variation CKX activity significantly correlated with yield many crop species while soybean ( Glycine max L.), possible associations...
Targeted metabolite analysis in combination with 13C-tracing is a convenient strategy to determine pathway activity biological systems; however, limited by challenges separating and detecting intermediates current chromatographic methods. Here, hydrophilic interaction chromatography tandem mass spectrometry approach was developed for improved separation, isotopologue analysis, quantification. The physiological responses of Yarrowia lipolytica strain engineered produce ∼400 mg/L α-ionone...
The combination of 13C-isotopic labeling and mass spectrometry imaging (MSI) offers an approach to analyze metabolic flux in situ. However, combining isotopic MSI presents technical challenges ranging from sample preparation, label incorporation, data collection, analysis. Isotopic individually create large, complex sets, this is compounded when both methods are combined. Therefore, analyzing isotopically labeled requires streamlined procedures support biologically meaningful...
The capacity to leverage high resolution mass spectrometry (HRMS) with transient isotope labeling experiments is an untapped opportunity derive insights on context-specific metabolism, that difficult assess quantitatively. Tools are needed comprehensively mine isotopologue information in automated, high-throughput way without errors. We describe a tool, Stable Isotope-assisted Metabolomics for Pathway Elucidation (SIMPEL), simplify analysis and interpretation of isotope-enriched HRMS...
Pollen germination is an essential process for pollen tube growth, pollination, and therefore seed production in flowering plants, it requires energy either from remobilization of stored carbon sources, such as lipids starches, or secreted exudates the stigma. Transcriptome analysis vitro previously showed that 14 GO terms, including metabolism energy, were overrepresented Arabidopsis . However, little understood about global changes carbohydrate energy-related metabolites during transition...
The analysis of stable isotope labeling experiments requires accurate, efficient, and reproducible quantification mass isotopomer distributions (MIDs), which is not a core feature general-purpose metabolomics software tools that are optimized to quantify metabolite abundance. Here, we present PIRAMID (Program for Integration Rapid Analysis Mass Isotopomer Distributions), MATLAB-based tool addresses this need by offering user-friendly, graphical user interface-driven program automate the...
ACYL CARRIER PROTEIN4 (ACP4) is the most abundant ACP isoform in Arabidopsis (Arabidopsis thaliana) leaves and acts as a scaffold for de novo fatty acid biosynthesis substrate acyl-ACP-utilizing enzymes. Recently, ACP4 was found to interact with protein-designated plastid RHOMBOID LIKE10 (RBL10) that affects chloroplast monogalactosyldiacylglycerol (MGDG) biosynthesis, but cellular function of this interaction remains be explored. Here, we generated characterized acp4 rbl10 double mutants...
Abstract Plant leaf biomass is composed predominantly of carbohydrate and protein with less than 5% dry weight allocated to lipid 1% total in the form triacylglycerols (TAGs). The combined overexpression multiple genes involved different aspects TAG synthesis stabilization can result accumulation over 30% tobacco leaves, presumably requiring many metabolic adjustments within plant cells. consequences source sink capacities high oil accumulating transgenic leaves compared wild-type were...
Lipids represent the most diverse pool of metabolites found in cells, facilitating compartmentation, signaling, and other functions. Dysregulation lipid metabolism is linked to disease states such as cancer neurodegeneration. However, limited tools are available for quantifying metabolic fluxes across lipidome. To directly measure reaction encompassing compound homeostasis, we applied stable isotope tracing, liquid chromatography-high-resolution mass spectrometry, network-based isotopologue...
Abstract C 4 photosynthesis is an adaptive photosynthetic pathway which concentrates CO 2 around Rubisco in specialized bundle sheath cells to reduce photorespiration. Historically, the has been characterized into three different subtypes based on decarboxylase involved, although recent work provided evidence that some plants can use multiple decarboxylases, with maize particular using both NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PEPCK) pathway. Parallel pathways...
ABSTRACT The inverse correlation between protein and oil production in soybeans is well-documented; however, it has been based primarily on the composition of mature seeds. Though this cumulative result events over course soybean seed development, does not convey information specific to metabolic fluctuations during developmental growth regimes. Maternal nutrient supply via coat exudate measurements metabolite levels within cotyledon were assessed across development identify trends...
Root exudation refers to the processes by which plants release compounds called root exudates into soil. These are primarily carbon-containing that interact with microbial communities in rhizosphere. Microbial consumption of reduces concentration exudated soil, causing plant exude more those compounds. Currently, there is limited understanding interaction between plant-root mechanisms and surrounding communities. Among Sorghum Association Panel (SAP), an established genetically characterized...
Abstract Cytokinins (CKs) play a fundamental role in regulating dynamics of organ source/sink relationships during plant development, including flowering and seed formation stages. As result, CKs are key drivers yield. The cytokinin oxidase/dehydrogenase (CKX) is one the critical enzymes responsible for CK levels by causing their irreversible degradation. Variation CKX gene activity significantly correlated with yield many crop species while soybean ( Glycine max L.), possible associations...