A5031897194- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Plant responses to water stress
- Protein purification and stability
- Plant responses to elevated CO2
- Virus-based gene therapy research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Bioenergy crop production and management
- Fungal and yeast genetics research
Donald Danforth Plant Science Center
2019-2021
United States Department of Agriculture
2020-2021
Agricultural Research Service
2019-2021
Bristol-Myers Squibb (United States)
2020
The metabolic plasticity of tobacco leaves has been demonstrated via the generation transgenic plants that can accumulate over 30% dry weight as triacylglycerols. In investigating changes in carbon partitioning these high lipid-producing (HLP) leaves, foliar lipids accumulated stepwise development. Interestingly, non-transient starch was observed to with plant age WT but not HLP a drop concurrent an increase lipid content. tradeoff between and studied using 13CO2-labeling experiments...
Abstract Sorghum (Sorghum bicolor) and its relatives in the grass tribe Andropogoneae bear their flowers pairs of spikelets which one spikelet (seed-bearing or sessile [SS]) pair produces a seed other is sterile male (staminate). This division function does not occur major cereals such as wheat (Triticum aestivum) rice (Oryza sativa). Additionally, bract SS often long extension, awn, that same position as, but independently derived from, rice. The unknown awn has been tested Andropogoneae....
Acyl carrier proteins (ACPs) are the scaffolds for fatty acid biosynthesis in living systems, rendering them essential to a comprehensive understanding of lipid metabolism. However, accurate quantitative methods assess individual acyl-ACPs do not exist. We developed robust method quantify picogram level. successfully identified acyl-ACP elongation intermediates (3-hydroxyacyl-ACPs and 2,3-trans-enoyl-ACPs) unexpected medium-chain (C10:1, C14:1) polyunsaturated long-chain (C16:3) acyl-ACPs,...
Abstract Viral safety is required for biological products to treat human diseases, and the burden of inactivation or virus removal lies on downstream purification process. Minute mice (MVM) a nonenveloped parvovirus commonly used as worst‐case model in validation studies because its small size high chemical stability. In this study, we investigated use MVM‐mock particle (MVP) bacteriophage ΦX174 surrogates MVM mimic viral clearance studies, with focus chromatography operations. Based...
Abstract Plant leaf biomass is composed predominantly of carbohydrate and protein with less than 5% dry weight allocated to lipid 1% total in the form triacylglycerols (TAGs). The combined overexpression multiple genes involved different aspects TAG synthesis stabilization can result accumulation over 30% tobacco leaves, presumably requiring many metabolic adjustments within plant cells. consequences source sink capacities high oil accumulating transgenic leaves compared wild-type were...
Abstract C 4 photosynthesis is an adaptive photosynthetic pathway which concentrates CO 2 around Rubisco in specialized bundle sheath cells to reduce photorespiration. Historically, the has been characterized into three different subtypes based on decarboxylase involved, although recent work provided evidence that some plants can use multiple decarboxylases, with maize particular using both NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PEPCK) pathway. Parallel pathways...
ABSTRACT Acyl carrier proteins (ACPs) are the scaffolds for fatty acid biosynthesis in living systems, rendering them essential to a comprehensive understanding of lipid metabolism; however, accurate quantitative methods assess individual acyl-ACPs do not exist. A robust method was developed quantify at picogram levels. Acyl-ACP elongation intermediates (3-hydroxyacyl-ACPs and 2, 3- trans -enoyl-ACPs), unexpected medium chain (C10:1, C14:1) polyunsaturated long (C16:3) were also identified,...