A5034890830- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- Soybean genetics and cultivation
- Nitrogen and Sulfur Effects on Brassica
- Plant Molecular Biology Research
- Phytase and its Applications
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Agricultural pest management studies
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Lipid metabolism and biosynthesis
- Biochemical Analysis and Sensing Techniques
- Steroid Chemistry and Biochemistry
- Studies on Chitinases and Chitosanases
- Plant Stress Responses and Tolerance
- Proteins in Food Systems
- Plant Pathogenic Bacteria Studies
- Potato Plant Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- GABA and Rice Research
- Biochemical and Molecular Research
- Folate and B Vitamins Research
- Plant Micronutrient Interactions and Effects
Agriculture and Agri-Food Canada
2015-2024
London Research and Development Centre
2015-2024
Western University
2014-2023
Vanderbilt University
2022
Donald Danforth Plant Science Center
2022
Agricultural Research Service
2022
Vanderbilt Health
2022
National Association of Friendship Centres
2017
Concordia University
1997-2006
Université Laval
1995-1999
Here we demonstrate that GmMYB176 regulates CHS8 expression and affects isoflavonoid synthesis in soybean. We previously established determines the level soybean seeds by comparing transcript profiles of cultivars with different contents. In present study, a functional genomic approach was used to identify factor synthesis. Candidate genes were cloned, co-transfection assays performed Arabidopsis leaf protoplasts. The results showed can trans-activate promoter maximum activity. Transient...
The nitrogen fixing ability of common bean (Phaseolus vulgaris L.) in association with rhizobia is often characterized as poor compared to other legumes, and fertilizers are commonly used production achieve high yields, which general inhibits fixation. In addition, plants cannot take up all the applied soil a fertilizer leading runoff groundwater contamination. overall objective this work reduce use production. This would be major advance profitability for industry Canada significantly...
Although pulses are nutritious foods containing high amounts of protein, fiber and phytochemicals, their consumption use in the food industry have been limited due to formation unappealing flavors/aromas described as beany, green, grassy. Lipoxygenase (LOX) enzymes prevalent among pulse seeds, activity can lead specific volatile organic compounds (VOCs) from certain polyunsaturated fatty acids (PUFAs). As a widespread issue legumes, including soybean, these VOCs linked taste perception...
Recent discoveries from brassinosteroid-deficient mutants led to the recognition that plants, like animals, use steroids regulate their growth and development. We describe characterization of one member a <i>Brassica napus</i>sulfotransferase gene family coding for an enzyme catalyzes the<i>O</i>-sulfonation brassinosteroids mammalian estrogenic steroids. The is specific hydroxyl group at position 22 with preference 24-epicathasterone, intermediate in biosynthesis 24-epibrassinolide....
The relationship between asparagine metabolism and protein concentration was investigated in soybean seed. Phenotyping of a population recombinant inbred lines adapted to Illinois confirmed positive correlation free levels developing seeds at maturity. Analysis second Ontario associated the elevated trait with two four quantitative loci determining variation for concentration, including major one on chromosome 20 (linkage group I) which has been reported multiple populations. In seed coat,...
The levels of nitric oxide (NO) and reactive oxygen species (ROS), ATP/ADP ratios, reduction ascorbate glutathione, expression the genes encoding proteins involved in metabolism NO activities enzymes fermentation ROS were studied embryos germinating seeds two barley (Hordeum vulgare L.) cultivars differing dormancy level. level production continuously increased after imbibition while nitrosylated SH-groups increased. This corresponded to decrease free proteins. At early stage germination...
Several approaches were taken to examine the role of N-assimilate supply in control soybean (Glycine max) seed composition. In first study, developing seeds grown vitro with D-[U-14C]sucrose (Suc) and different concentrations Gln. Light stimulated carbon flux into oil protein, was required sustain Suc uptake anabolic processes under conditions elevated nitrogen supply. High Gln resulted higher transcript levels β-conglycinin oleosin. second analyses soluble amino acid pools two genetically...
Abstract The endosperm provides nutrients and growth regulators to the embryo during seed development. LEAFY COTYLEDON1 (LEC1) has long been known be essential for maturation. LEC1 is expressed in both endosperm; however, functional relevance of endosperm-expressed development unclear. Here, we provide genetic transgenic evidence demonstrating that necessary sufficient We show endosperm-synthesized capable orchestrating full maturation absence embryo-expressed . Inversely, without expression...
The comparison of the deduced amino acid sequences plant and animal sulfotransferases (ST) has allowed identification four well conserved regions, previous experimental evidence suggested that regions I IV might be involved in binding cosubstrate, 3′-phosphoadenosine 5′-phosphosulfate (PAPS). Moreover, region is homologous to glycine-rich phosphate loop (P-loop) motif known nucleotide several protein families. In this study, function residues within these two was investigated by...
Common beans (Phaseolus vulgaris) form a relationship with nitrogen-fixing rhizobia and through process termed symbiotic nitrogen fixation (SNF) which provides them source of nitrogen. However, are considered poor fixers, modern production practices involve routine use N fertilizer, leads to the down-regulation SNF. High-yielding, conventionally-bred bean varieties developed using conventional selection criteria, typically not including SNF efficiency, may have lost this trait over decades...
The contents of sulfur amino acids in seeds common bean (Phaseolus vulgaris L.) are suboptimal for nutrition. They accumulate large amounts a γ-glutamyl dipeptide S-methyl-cysteine, nonprotein acid that cannot substitute methionine or cysteine the diet. Protein accumulation and composition were characterized three genetically related lines integrating progressive deficiency major seed storage proteins, phaseolin, phytohemagglutinin, arcelin. Nitrogen, carbon, comparable among lines....
It has been hypothesized that the relatively low concentration of sulfur amino acids in legume seeds might be an ecological adaptation to nutrient poor, marginal soils. SARC1 and SMARC1N-PN1 are genetically related lines common bean (dry bean, Phaseolus vulgaris) differing seed storage protein composition. In SMARC1N-PN1, lack phaseolin major lectins is compensated by increased levels sulfur-rich proteins, resulting enhanced cysteine methionine, mostly at expense abundant non-protein acid,...
Abstract SPT6 is a conserved elongation factor that associated with phosphorylated RNA polymerase II (RNAPII) during transcription. Recent transcriptome analysis in yeast mutants revealed its potential role the control of transcription initiation at genic promoters. However, mechanism by which this achieved and how linked to remains be elucidated. Here, we present genome-wide occupancy Arabidopsis SPT6-like (SPT6L) demonstrate facilitating RNAPII across transcribed genes. We also further...
Abstract Background A key issue for implementation of CRISPR-Cas9 genome editing plant trait improvement and gene function analysis is to efficiently deliver the components, including guide RNAs (gRNAs) Cas9, into plants. Plant virus-based gRNA delivery strategy has proven be an important tool editing. However, its application in soybean which crop not been reported yet. ALSV ( apple latent spherical virus ) highly infectious could explored delivering elements Results To develop a ALSV-based...
Abstract Background A deficiency in phaseolin and phytohemagglutinin is associated with a near doubling of sulfur amino acid content genetically related lines common bean ( Phaseolus vulgaris ), particularly cysteine, elevated by 70%, methionine, 10%. This mostly takes place at the expense an abundant non-protein acid, S -methyl-cysteine. The mainly compensated increased levels 11S globulin legumin residual lectins. Legumin, albumin-2, defensin albumin-1 were previously identified as...