A5029481202- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Natural product bioactivities and synthesis
- Antioxidant Activity and Oxidative Stress
- Carbohydrate Chemistry and Synthesis
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Enzyme Production and Characterization
- Synthetic Organic Chemistry Methods
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Microbial Metabolism and Applications
- Peptidase Inhibition and Analysis
- Marine Sponges and Natural Products
- Chemical Synthesis and Analysis
- Enzyme Catalysis and Immobilization
- Polyamine Metabolism and Applications
- Biochemical and biochemical processes
- Crystallization and Solubility Studies
The University of Tokyo
2016-2025
Biotechnology Research Center
2004-2016
Howard Hughes Medical Institute
2012
University of Illinois Urbana-Champaign
2012
Hokkaido University
2011
Salk Institute for Biological Studies
2005
Tokyo University of Agriculture
1990-2003
Kirin (Japan)
2001
Tokyo Institute of Technology
2001
University of Minnesota
2001
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded biosynthetic gene clusters. Information about these clusters, is currently dispersed throughout the literature, making it difficult exploit. To facilitate consistent systematic deposition retrieval data on we propose Minimum a Biosynthetic Gene cluster (MIBiG) standard.
Significance Terpenes are generally considered to be plant or fungal metabolites, although a small number of odoriferous terpenes bacterial origin have been known for many years. Recently, extensive genome sequencing and bioinformatic analysis deduced proteins using profile based on hidden Markov model revealed 262 distinct predicted terpene synthases. Although these presumptive synthase genes seem silent in their parent microorganisms, controlled expression an engineered heterologous...
Several eubacteria including Esherichia coli use an alternative nonmevalonate pathway for the biosynthesis of isopentenyl diphosphate instead ubiquitous mevalonate pathway. In pathway, 2- C -methyl- d -erythritol or its 4-phosphate, which is proposed to be formed from 1-deoxy- -xylulose 5-phosphate via intramolecular rearrangement followed by reduction process, one biosynthetic precursors diphosphate. To clone gene(s) responsible synthesis we prepared and selected E. mutants with obligatory...
Gibberellins (GAs) are diterpene plant hormones essential for many developmental processes. Although the GA biosynthesis pathway has been well studied, our knowledge on its early stage is still limited. There two possible routes of isoprenoids leading to GAs, mevalonate (MVA) in cytosol and methylerythritol phosphate (MEP) plastids. To distinguish these possibilities, metabolites from each isoprenoid were selectively labeled with<sup>13</sup>C <i>Arabidopsis</i> seedlings. Efficient...
Abstract The discovery of the 2-C-methyl-d-erythritol-4-phosphate pathway for biosynthesis isoprenoids raises important question nature and regulation enzymes involved in this pathway.CLA1, a gene previously isolated from Arabidopsis, encodes first enzyme pathway, 1-deoxy-d-xylulose-5-phosphate synthase. We demonstrate activity by complementation cla1-1mutant phenotype direct enzymatic assays. Based on mRNA protein expression patterns is expressed mainly developing photosynthetic...
Genome mining is a promising method to discover novel secondary metabolites in the postgenomic era. We applied Aspergillus oryzae heterologous expression system functionally characterize cryptic bifunctional terpene synthase genes found fungal genomes and identified sesterfisherol gene (NfSS) from Neosartorya fischeri. Sesterfisherol contains characteristic 5-6-8-5 tetracyclic ring modified by cytochrome P450 monooxygenase (NfP450) sesterfisheric acid. The cyclization mechanism was proposed...
Versipelostatin (VST) is an unusual 17-membered macrocyclic polyketide product that contains a spirotetronate skeleton. In this study, the entire VST biosynthetic gene cluster (vst) spanning 108 kb from Streptomyces versipellis 4083-SVS6 was identified by heterologous expression using bacterial artificial chromosome vector. Here, we demonstrate enzyme, VstJ, catalyzes stereoselective [4+2]-cycloaddition between conjugated diene and exocyclic olefin of newly tetronate-containing intermediate...
Abstract Terpene cyclization reactions are fascinating owing to the precise control of connectivity and stereochemistry during catalytic process. Cyclooctat‐9‐en‐7‐ol synthase (CotB2) synthesizes an unusual 5‐8‐5 fused‐ring structure with six chiral centers from universal diterpene precursor, achiral C 20 geranylgeranyl diphosphate substrate. An new mechanism for exquisite CotB2‐catalyzed that involves a carbon–carbon backbone rearrangement three long‐range hydride shifts is proposed, based...
A gene cluster encoding five enzymes of the mevalonate pathway had been cloned from Streptomyces sp. strain CL190. This contained an additional ORF, orfD , unknown protein that was detected in some archaebacteria and Gram-positive bacteria including Staphylococcus aureus . The recombinant product purified as a soluble characterized. molecular mass enzyme estimated to be 37 kDa by SDS-polyacrylamide gel electrophoresis 155 filtration chromatography, suggesting is most likely tetramer. flavin...
Many waterbodies across the United States do not meet water quality standards. To help determine where and to what extent improvements should be sought, policymakers must consider costs of regulations with their monetized values. We ...Scientific knowledge related quantifying benefits for landscape-wide does current regulatory benefit–cost analysis needs in States. In this study we addressed gap by ...
Recent studies described several different routes that facilitate nitrogen-nitrogen bond formation in natural product biosynthesis. We report herein the identification of unprecedented machinery for hydrazine involved biosynthesis s56-p1, a dipeptide with unique hydrazone unit. The gene cassette comprising this is widespread across bacterial phyla, highlighting overlooked potential bacteria to synthesize hydrazine.
ABSTRACT In addition to the ubiquitous mevalonate pathway, Streptomyces sp. strain CL190 utilizes nonmevalonate pathway for isopentenyl diphosphate biosynthesis. The initial step of this is formation 1-deoxy- d -xylulose 5-phosphate (DXP) by condensation pyruvate and glyceraldehyde 3-phosphate catalyzed DXP synthase. corresponding gene, dxs , was cloned from using PCR with two oligonucleotide primers synthesized on basis highly conserved regions among homologs six genera. gene encodes 631...