A5059777922- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Marine Sponges and Natural Products
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Fungal Biology and Applications
- Chemical Synthesis and Analysis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Synthesis and Biological Activity
- Cancer therapeutics and mechanisms
- Bioactive Compounds and Antitumor Agents
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- Natural product bioactivities and synthesis
- Synthetic Organic Chemistry Methods
- Biochemical and Structural Characterization
- Plant-Microbe Interactions and Immunity
- Mycobacterium research and diagnosis
- Plant Pathogens and Fungal Diseases
- Actinomycetales infections and treatment
Microbial Chemistry Research Foundation
2016-2025
Kyoto University
2015
Institute of Molecular Biology and Biophysics
2013
Tokyo University of Science
2010
Keio University
1958-2008
Renesas Electronics (United States)
2008
The University of Tokyo
1997
Jichi Medical University
1996
Hokkaido University
1993
Central Research Laboratories (United Kingdom)
1993
Abstract Terpene cyclization reactions are fascinating owing to the precise control of connectivity and stereochemistry during catalytic process. Cyclooctat‐9‐en‐7‐ol synthase (CotB2) synthesizes an unusual 5‐8‐5 fused‐ring structure with six chiral centers from universal diterpene precursor, achiral C 20 geranylgeranyl diphosphate substrate. An new mechanism for exquisite CotB2‐catalyzed that involves a carbon–carbon backbone rearrangement three long‐range hydride shifts is proposed, based...
Cyclopropanol rings are highly reactive and may function as molecular "warheads" that affect natural product bioactivity. Yet, knowledge on their biosynthesis is limited. Using gene cluster analyses, isotope labeling, in vitro enzyme assays, we shed first light the of cyclopropanol-substituted amino acid cleonine, a residue antimicrobial depsipeptide valgamicin C cytotoxic glycopeptide cleomycin A2. We decipher biosynthetic origin show cleonine cyclopropanol ring derived from...
Two-component signal transduction systems (TCSs) in prokaryotes often regulate gene clusters that induce pathogenicity, and thus they have frequently been proposed as potential drug targets for attenuating the virulence of pathogens. The pathogenic Streptococcus mutans, major etiological pathogen dental caries, is also regulated by its TCSs. object this study was to evaluate effect a histidine kinase (HK) inhibitor against two factors S. mutans: biofilm formation acid tolerance. Walkmycin C...
The total synthesis of the 20 homogeneous members mannosylerythritol lipids (MELs) with different alkyl chain lengths was effectively and systematically accomplished from a strategically designed common key intermediate that stereoselectively constructed by borinic acid catalyzed β-mannosylation reaction. In addition, their antibacterial activities against Gram-positive bacteria were evaluated. Our results demonstrated not only length chains but also pattern Ac groups on mannose moiety...
Non-tuberculous mycobacteria (NTM) is gaining clinical recognition as a recently emerging pulmonary pathogen. Mycobacterium avium complex (MAC), the most common NTM, cause of MAC disease. Currently, macrolide azithromycin (AZM) standard first-line antibiotic for treatment However, rise drug-resistant necessitates development alternative therapeutics. Here, we present late-stage boron-mediated aglycon delivery strategy selective modification AZM, generating library potential anti-MAC drugs...
Anti-apoptotic oncoproteins Bcl-2 and Bcl-xL are overexpressed in many cancers play a crucial role cancer initiation, progression, resistance to chemotherapy. Therefore, the discovery of functional inhibitor for these proteins improved understanding molecular mechanisms will be an aid novel anti-tumor therapies. Here, using chemical−genetic cell-based screening, we have discovered chemically biologically unique substance, incednine, as modulator Bcl-2/Bcl-xL from fermentation broth...
By screening cultures of soil bacteria, we re-discovered an old antibiotic (nybomycin) as with a novel feature. Nybomycin is active against quinolone-resistant Staphylococcus aureus strains mutated gyrA genes but not those intact which quinolone antibiotics are effective. Nybomycin-resistant mutant were generated from quinolone-resistant, nybomycin-susceptible, vancomycin-intermediate S. (VISA) strain Mu 50. The mutants, occurring at extremely low rate (<1 × 10(-11)/generation), found to...
ABSTRACT The WalK (histidine kinase)/WalR (response regulator) two-component signal transduction system is a master regulatory for cell wall metabolism and growth. This conserved in low G+C Gram-positive bacteria, including Bacillus subtilis , Staphylococcus aureus Enterococcus faecalis Streptococcus mutans . In this study, we found the first antibiotic that functions as inhibitor (signermycin B) by screening 10,000 Streptomyces extracts. chemical structure (C 23 H 35 NO 4 ; molecular...
Abstract Terpene cyclization reactions are fascinating owing to the precise control of connectivity and stereochemistry during catalytic process. Cyclooctat‐9‐en‐7‐ol synthase (CotB2) synthesizes an unusual 5‐8‐5 fused‐ring structure with six chiral centers from universal diterpene precursor, achiral C 20 geranylgeranyl diphosphate substrate. An new mechanism for exquisite CotB2‐catalyzed that involves a carbon–carbon backbone rearrangement three long‐range hydride shifts is proposed, based...
Tripropeptin C (TPPC) is a naturally occurring cyclic lipodepsipeptide antibiotic produced by Lysobacter sp. TPPC exhibits potent antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and penicillin-resistant Streptococcus pneumoniae. This also inhibits the incorporation of N-acetylglucosamine into peptidoglycan S. at 50% inhibitory concentration (IC(50)) 0.7 μM, which proportional to its MIC (0.87 μM; equivalent 1.0...
Abstract The abuse of antibacterial drugs imposes a selection pressure on bacteria that has driven the evolution multidrug resistance in many pathogens. Our efforts to discover novel classes antibiotics combat these pathogens resulted discovery amycolamicin (AMM). absolute structure AMM was determined by NMR spectroscopy, X‐ray analysis, chemical degradation, and modification its functional groups. consists trans ‐decalin, tetramic acid, two unusual sugars (amycolose amykitanose),...