A5015611641- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Health disparities and outcomes
- Aging and Gerontology Research
- Cancer Genomics and Diagnostics
- Migration, Aging, and Tourism Studies
- Cancer Cells and Metastasis
- Metastasis and carcinoma case studies
- Microfluidic and Capillary Electrophoresis Applications
- Health and Wellbeing Research
- Gastric Cancer Management and Outcomes
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- Anodic Oxide Films and Nanostructures
- Aortic Disease and Treatment Approaches
- Genetic factors in colorectal cancer
- Geriatric Care and Nursing Homes
- Identity, Memory, and Therapy
- Genetic and Kidney Cyst Diseases
- Intergenerational Family Dynamics and Caregiving
- Hepatocellular Carcinoma Treatment and Prognosis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Glycosylation and Glycoproteins Research
Tokyo Metropolitan Institute of Gerontology
2018-2024
Waseda University
2020-2024
Hitachi (Japan)
1996-2020
Kindai University
2011-2019
Aichi Health Promotion Foundation
2019
Tokyo Medical and Dental University
2014
Kumamoto University
2012-2013
Tokushima University
1997-2003
Iowa State University
2002-2003
Ames National Laboratory
2002
ABSTRACT Background: Social isolation and homebound statuses are possible risk factors for increased mortality among older adults. However, no study has addressed the impact of accumulation these two on mortality. The aim this was to examine whether such all-cause Methods: analyzed sample drawn from a mail survey 1,023 adults without instrumental activities daily living disability. Participants were classified into four groups according frequency both face-to-face non-face-to-face...
Two-component signal transduction systems (TCSs) in prokaryotes often regulate gene clusters that induce pathogenicity, and thus they have frequently been proposed as potential drug targets for attenuating the virulence of pathogens. The pathogenic Streptococcus mutans, major etiological pathogen dental caries, is also regulated by its TCSs. object this study was to evaluate effect a histidine kinase (HK) inhibitor against two factors S. mutans: biofilm formation acid tolerance. Walkmycin C...
Abstract Background Small cell carcinoma of the uterine cervix (SCCC) is a rare and highly malignant human papillomavirus (HPV)‐associated cancer in which genes related to integration site can serve as target for precision medicine. The aim our study was establish workflow medicine HPV‐associated using patient‐derived organoid. Methods Organoid established from biopsy patient diagnosed with HPV18‐positive SCCC. Therapeutic targets were identified by whole exome sequencing (WES) RNA‐seq...
This study aims to identify the independent influence of face-to-face contact (FFC) and non-face-to-face (NFFC) on subsequent decline in self-rated health mental status by age. A total 12,000 participants were randomly selected among residents area, 1751 them responded both 2016 2018 mail surveys. The subsequently classified into three age groups (25–49: Young adults; 50–64: Mid-aged 65–84: Older adults). Social was assessed computing frequencies FFC NFFC. Multiple logistic regression...
We have developed a novel high-performance quantitative assay for unamplified nucleic acids that is based on single-molecule imaging. The apparatus simple but highly sensitive detection system uses normal CCD camera instead of an image-intensified camera. After the DNA molecules in sample were labeled with YOYO-1, they induced to migrate electrophoretically polymer solution and imaged. No chemical or biochemical amplification was required. Direct quantitation by counting possible, because...
Analysis of single-cell gene expression promises a more precise understanding molecular mechanisms living system. Most techniques only allow studies the expressions for limited numbers species. When amplification cDNA was carried out analysing genes, biases were frequently reported. A non-biased and efficient global-amplification method, which uses library immobilized on beads, developed entire single cells. Every step in this analysis from reverse transcription to optimized. By removing...
The health benefits of paid employment in late life are understood, but they might vary according to work motives. We examined the effects among older adults, focusing on motivation.We carried out a 2-year longitudinal survey from 2013 (baseline) 2015 (follow up). Among 7608 analysis included 1069 who completed both surveys and were employed at baseline. Work motives assessed using questionnaire. Participants assigned groups based their responses: (i) financial reasons; (ii) motivation other...
Single-cell RNA-seq is a powerful tool for revealing heterogeneity in cancer cells. However, each of the current single-cell platforms has inherent advantages and disadvantages. Here, we show that combining different can be an effective approach to obtaining complete information about expression differences sufficient cellular population understand transcriptional cancers. We demonstrate it possible estimate missing information. further even cases where precise individual gene cannot...
Abstract Spatial transcriptomics is useful for understanding the molecular organization of a tissue and providing insights into cellular function in morphological context. In order to obtain reproducible results spatial transcriptomics, we have maintain morphology RNA molecule stability during image acquisition biomolecule collection processes. Here, developed processing method robust RNA-seq from microdissection samples. this method, suppressed degradation fresh-frozen specimens by...
Abstract Spatial transcriptome analysis of formalin-fixed paraffin-embedded (FFPE) tissues using RNA-sequencing (RNA-seq) provides interactive information on morphology and gene expression, which is useful for clinical applications. However, despite the advantages long-term storage at room temperature, FFPE may be severely damaged by methylene crosslinking provide less than fresh-frozen tissues. In this study, we proposed a sensitive micro-tissue RNA-seq method that combines punching tissue...
ABSTRACT Gene expression analysis at the single-cell level by next-generation sequencing has revealed existence of clonal dissemination and microheterogeneity in cancer metastasis. The current spatial technologies can elucidate heterogeneity cell–cell interactions situ. To reveal regional expressional primary tumors metastases, we performed transcriptomic microtissues dissected from a triple-negative breast (TNBC) cell line MDA-MB-231 xenograft model with our automated tissue microdissection...
Aim Identifying factors that influence participation in social group activities older adults might be helpful promoting this population. We examined related to new or continuous according type. Methods carried out a community‐based, longitudinal study with 2‐year follow‐up period. Of 7608 aged ≥65 years, 3380 completed the survey and were included analysis. private (hobbies, sports volunteering; PrG) public (neighborhood associations senior citizen clubs; PuG) activity. Results For PrG,...
OBJECTIVE To elucidate the significance of clinical and immunogenic heterogeneity in Japanese children with insulin-dependent diabetes mellitus (IDDM). RESEARCH DESIGN AND METHODS Serial changes prevalence islet cell antibodies (ICAs) ICA titer were monitored for 10 years after diagnosis 34 IDDM children, 17 abrupt onset slow onset, whose durations disease > 5 years. RESULTS In slow-onset enough β-cell function was maintained early phase within 2 diagnosis. There a high ICAs both...
The central nucleus of the amygdala (CeA) and lateral division bed stria terminalis (BNST) are two major nuclei extended that plays essential roles in threat processing, responsible for emotional states such as fear anxiety. While some studies suggested functional differences between these nuclei, others showed anatomical neurochemical similarities. Despite their complex subnuclear organization, subnuclei-specific impact on behavior underlying molecular profiles remain obscure. We here...
Abstract Human papillomavirus 18 (HPV18) is a highly malignant HPV genotype among high‐risk HPVs, characterized by the difficulty of detecting it in precancerous lesions and its high prevalence adenocarcinomas. The cellular targets molecular mechanisms underlying infection remain unclear. In this study, we aimed to identify cells targeted HPV18 elucidate replication. Initially, established lentiviral vector (HPV18LCR‐GFP vector) containing long control region promoter located upstream EGFP ....
A blood assay for detection of lung cancer biomarkers could significantly improve patient prognosis and survival rates. Amplified fragment length polymorphism-differential display (AFLP-DD) was used to identify gene transcripts found in tissue the peripheral patients. The clones were evaluated expression tissue, patients healthy volunteers' blood. isolated transcript be from syndecan 1 gene, collagen 2 novel genes. All 4 expressed normal cultured primary cells 6 cell lines. RNA samples 69...
Background Homeostasis of the vessel wall is cooperatively maintained by endothelial cells (ECs), smooth muscle cells, and adventitial fibroblasts. The genetic deletion fibulin-4 (Fbln4) in (SMKO) leads to formation thoracic aortic aneurysms with disruption elastic fibers. Although Fbln4 expressed entire wall, its function ECs relevance maintenance valvulo-arterial integrity are not fully understood. Methods Results Gene silencing FBLN4 was conducted on human evaluate morphological changes...
Abstract: An indocyanine green (ICG) derivative (ICG N‐hydroxy sulfosuc‐cinimide ester; ICG‐sulfo‐OSu) has been developed as an antibody labeling substance suitable for vital immunohistochemical staining. However, appropriate fluorescence imaging system ICG‐sulfo‐OSu using infrared rays not yet reported. Therefore, we such a system. The absorption maxima of ICG and in buffer solution are both at 795 nm, the excitation their spectra 768 emission 807 nm. filter with transmission 710‐790 nm...
We studied the fundamental instrumental issues relevant to a capillary-based integrated system measure expression of specific gene directly from cells. Samples were introduced into capillary by use syringe pump. All reactions carried out in microthermocycler, where part having 1 μL inner volume was used as reaction vessel. First, cells lysed heating release RNA, followed deoxyribonuclease (DNase) treatment. Then, reverse transcription-polymerase chain (RT-PCR) performed obtain amplified...