A5103012219- Neuroscience and Neuropharmacology Research
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- Connective tissue disorders research
- Renin-Angiotensin System Studies
- Organ Transplantation Techniques and Outcomes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiac Valve Diseases and Treatments
- Amyotrophic Lateral Sclerosis Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Epilepsy research and treatment
- Cell Image Analysis Techniques
- RNA regulation and disease
- Barrier Structure and Function Studies
- Molecular Biology Techniques and Applications
- Linguistics and Discourse Analysis
- Ion channel regulation and function
- Glaucoma and retinal disorders
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Williams Syndrome Research
- Olfactory and Sensory Function Studies
- Blood Coagulation and Thrombosis Mechanisms
- Advanced X-ray and CT Imaging
- Neuropeptides and Animal Physiology
Waseda University
2021-2024
University of Tsukuba
2019-2024
Seinan Gakuin University
2022
Tokyo Medical and Dental University
2017-2021
The University of Tokyo
2021
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA)
2020
Kobe Gakuin University
2006-2008
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM cleared specimens), low-cost ($20,000-50,000), low-expertise (one-day installation by non-expert), yet practical do-it-yourself microscope as solution this bottleneck. Even the most fundamental configuration enables multi-color imaging whole mouse brains and...
Abnormal mechanosensing of smooth muscle cells (SMCs) resulting from the defective elastin-contractile units has been suggested to drive formation thoracic aortic aneurysms; however, precise molecular mechanism not elucidated.
Glutamate-mediated excitotoxicity induces neuronal death by altering various intracellular signaling pathways and is implicated as a common pathogenic pathway in many neurodegenerative diseases. In the case of motor neuron disease, there significant evidence to suggest that overactivation AMPA receptors due deficiencies expression function glial glutamate transporters GLT1 GLAST plays an important role mechanisms death. However, causal for transporter dysfunction remains unknown. Here, we...
Abstract Glial glutamate transporter GLT1 plays a key role in the maintenance of extracellular homeostasis. Recent human genetic studies have suggested that de novo mutations (EAAT2) cause early‐onset epilepsy with multiple seizure types. Consistent these findings, global null mice show lethal spontaneous seizures. The consequences dysfunction vary between different brain regions, suggesting may also regions. In this study, we generated region‐specific knockout by crossing floxed‐GLT1...
Abstract Cortical spreading depression (CSD) is a pathological neural excitation that underlies migraine pathophysiology. Since glutamate receptor antagonists impair CSD propagation, susceptibility to might be determined by any of the neuronal (excitatory amino acid carrier 1 [EAAC1]) and glial (GLutamate ASpartate Transporter [GLAST] transporter [GLT‐1]) transporters, which are responsible for clearing extracellular glutamate. To investigate this hypothesis, we performed...
Hereditary aortic diseases (hADs) increase the risk of dissections and ruptures. Recently, we have established an objective approach to measure rupture force murine aorta, thereby explaining outcomes clinical studies assessing added value approved drugs in vascular Ehlers-Danlos syndrome (vEDS). Here, applied our six additional mouse hAD models. We used two models (Fbn1C1041G Fbn1mgR ) Marfan (MFS) as well one smooth-muscle-cell-specific knockout (SMKO) Efemp2 three CRISPR/Cas9-engineered...
Angiotensin-converting enzyme (ACE) inhibitor enhances the liver regeneration in rats after partial hepatectomy (PH), though precise mechanisms are unknown. To determine roles of bradykinin and angiotensin II ACE inhibitor-induced enhancement regeneration, we investingated effects lisinopril (ACE inhibitor), candesartan losartan (angiotensin type 1 (AT1) receptor antagonists) icatibant (bradykinin B2 antagonist) on hepatic regenerative response to 70% PH rat. The was evaluated by measuring...
Angiotensin II (Ang II) is an important mediator stimulating liver fibrosis after injury. However, it not known whether Ang plays a role in regeneration. Here, we investigate the effects of type 1 (AT1) receptor blocker (ARB), angiotensin-converting enzyme inhibitor (ACEI), systemic infusion II, and genetic deficiency AT1a (AT1a-KO) on hepatic regenerative response to partial hepatectomy (PH) mice. Administration ARB (candesartan cilexetil losartan) or ACEI (enarapril lisinopril) enhanced...
Background Homeostasis of the vessel wall is cooperatively maintained by endothelial cells (ECs), smooth muscle cells, and adventitial fibroblasts. The genetic deletion fibulin-4 (Fbln4) in (SMKO) leads to formation thoracic aortic aneurysms with disruption elastic fibers. Although Fbln4 expressed entire wall, its function ECs relevance maintenance valvulo-arterial integrity are not fully understood. Methods Results Gene silencing FBLN4 was conducted on human evaluate morphological changes...
Our present study aimed to characterize the effects of lipopolysaccharide (LPS) on expression bradykinin B2-receptor in mouse heart, which may have a role cardiac depression during sepsis. We found that LPS induced up-regulation mRNA heart vivo and cultured myocytes vitro. Like LPS, tumor necrosis factor-α (TNF-α) but not interleukin (IL)-1-β, IL-6 or endothelin-1 stimulated myocytes. The effect was also mimicked by Ang II via type 1 (AT1-) receptor. Losartan, an AT1-receptor antagonist,...
Abstract Background Supravalvular aortic stenosis (SVAS) is one of the congenital cardiovascular diseases characterized by aorta. The stenotic lesions occur anywhere above valve in tree as well pulmonary arteries and eventually leads to circulatory failure. disease gene has been identified on elastin ( ELN ) two types SVAS have categorized; a familial type an isolated with de novo mutation. Methods Fluorescent In situ hybridization (FISH) analysis sequencing were performed two‐generation...
ABSTRACT OBJECTIVE Hereditary aortic diseases (hADs) increase the risk of dissections and ruptures. Recently, we have established an objective approach to measure rupture force murine aorta, thereby explaining outcomes clinical studies assessing added value approved drugs in vascular Ehlers-Danlos syndrome (vEDS). Here, applied our six additional mouse hAD models. APPROACH AND RESULT We used two models Marfan (MFS) as well one smooth-muscle-cell-specific knockout (SMKO) Efemp2 three...
Background: Marfan syndrome (MFS) is a genetic disorder caused by mutations in the gene encoding theextracellular matrix Fibrillin-1 (FBN1), resulting systemic connective tissue abnormalities. The mostcommon life-threatening conditions are aortic rupture and/or dissection. Aortic dissections show highmortality rate, but details of molecular triggers unknown, particularly acute dissection(AAD). Raman spectroscopy technique for analyzing structures from spectra. It hasrecently attracted...
Aortic rupture and dissection are life-threatening complications of ascending thoracic aortic aneurysms (aTAAs) the risk assessment has been largely based on monitoring a lumen size enlargement. Temporal changes extracellular matrix (ECM), which critical influence remodeling, not routinely evaluated nor cardiovascular biomarkers exist to predict aTAA formation. Here, we performed Raman microspectroscopy imaging identified marker spectra specific for aTAAs in mice humans by multivariate data...
Morphological inflection is known to be difficult master for L2 learners. In this paper, we examine the state of use in verbal tense system among learners French, and contrast it with FFL textbooks. The objectives our study are threefold: 1) To establish distribution tenses on French textbooks an automatic way, order obtain first fully empirical extensive resource tenses; 2) objectively describe by different CEFR levels; 3) identify that struggle with. Through description learners, found...