A5022189088- Spectroscopy Techniques in Biomedical and Chemical Research
- 3D Printing in Biomedical Research
- Spectroscopy and Chemometric Analyses
- Molecular Biology Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Proteoglycans and glycosaminoglycans research
- Tissue Engineering and Regenerative Medicine
- Microfluidic and Bio-sensing Technologies
- Retinal Diseases and Treatments
- Infective Endocarditis Diagnosis and Management
- Signaling Pathways in Disease
- Transplantation: Methods and Outcomes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Retinal and Optic Conditions
- Aortic aneurysm repair treatments
- Cardiac Valve Diseases and Treatments
- Cancer Cells and Metastasis
- Diabetes and associated disorders
- Retinal Development and Disorders
- Muscle Physiology and Disorders
- Collagen: Extraction and Characterization
- Cerebrospinal fluid and hydrocephalus
- Organic Light-Emitting Diodes Research
- Cardiac Fibrosis and Remodeling
University of Tübingen
2016-2025
Natural and Medical Sciences Institute
2019-2024
University Children's Hospital Tübingen
2021
Women's Health Research Institute
2021
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2017
Institut für Frauengesundheit
2016
The increasing prevalence of diabetes, its heterogeneity, and the limited number treatment options drive need for physiologically relevant assay platforms with human genetic background that have potential to improve mechanistic understanding e\xpedite diabetes-related research treatment. In this study, we developed an endocrine pancreas-on-a-chip model based on a tailored microfluidic platform, which enables self-guided trapping single pseudo-islets. Continuous, low-shear perfusion provides...
(1) Background: Cervical intraepithelial neoplasia (CIN) of long-term persistence or associated with individual treatment indications often requires highly invasive treatments. These are risks bleeding, infertility, and pregnancy complications. For low- middle-income countries (LMICs), standard procedures difficult to implement manage. We characterized the application energized gas "noninvasive physical plasma" (NIPP) for tissue devitalization CIN. (2) Methods: report establishment a...
Abstract Luminescence thermometry represents a technique of choice for measurements in small objects and imaging temperature distribution. However, most state‐of‐the‐art luminescent probes are limited spectral characteristics, brightness, photostability, sensitivity. Molecular thermometers the new generation utilizing air moisture‐stable zirconium(IV) pyridinedipyrrolide complexes can address all these limitations. The dyes emit pure thermally activated delayed fluorescence without any...
Fibrosis is a consequence of the pathological remodeling extracellular matrix (ECM) structures in connective tissue an organ. It often caused by chronic inflammation, which over time, progressively leads to excess deposition collagen type I (COL I) that replaces healthy structures, many cases leaving stiff scar. Increasing fibrosis can lead organ failure and death; therefore, developing methods potentially allow real-time monitoring early onset or progression are highly valuable. In this...
Significance Macrophage plasticity and activation dynamics are under intense investigation because their full complexity cannot be captured by the few predefined markers that commonly used. Macrophages highly adherent respond to surface microstructures in a sensitive manner. Here, we use Raman microspectroscopy imaging study macrophage response defined stimuli transfer these findings macrophages cultured on titanium with varying roughness. We show Raman-based methods can discriminate between...
Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved tissue reorganization. However, contrast to genomic analysis, the actual biomolecular composition sample has fallen behind, leaving a gap potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable filling this gap. In study we demonstrate spatially...
In the past decades, vaccine development has made great strides. Nevertheless, more often than not, candidates fail in advanced stages of and clinical trials. A key reason is poor predictive value non-clinical vivo vitro models, due to either species-specific differences immune response or insufficient reflection physiological mechanisms. Reliable modeling human adaptive responses a prerequisite understand processes leading vaccine-induced protective immunization drive informed decisions...
Smooth muscle cell (SMC) diversity and plasticity are limiting factors in their characterization application cardiovascular tissue engineering. This work aimed to evaluate the potential of Raman microspectroscopy imaging distinguish SMCs different origins phenotypes. Cultured human isolated from vascular non-vascular tissues as well fixed SMC-containing were analyzed. In addition, spectra images tissue-engineered SMC constructs acquired. Routine techniques such qPCR, histochemistry,...
Abstract Ischemia impacts multiple organ systems and is the major cause of morbidity mortality in developed world. disrupts tissue homeostasis, driving cell death, damages structure integrity. Strategies to heal organs, like infarcted heart, or replace cells, as done pancreatic islet β ‐cell transplantations, are often hindered by ischemic conditions. Here, it discovered that basement membrane glycoprotein nidogen‐1 attenuates apoptotic effect hypoxia cardiomyocytes ‐cells via α v 3 integrin...
In situ heart valve tissue engineering is an emerging approach in which resorbable, off-the-shelf available scaffolds are used to induce endogenous restoration. Such designed recruit cells vivo, subsequently resorb polymer and produce remodel new valvular situ. Recently, preclinical studies using electrospun supramolecular elastomeric grafts have shown that this enables regeneration of pulmonary valves with long-term functionality vivo. However, the evolution mechanisms inflammation,...
Three-dimensional (3D) organoid culture recapitulating patient-specific histopathological and molecular diversity offers great promise for precision medicine in cancer. In this study, we established label-free imaging procedures, including Raman microspectroscopy (RMS) fluorescence lifetime microscopy (FLIM), situ cellular analysis metabolic monitoring of drug treatment efficacy. Primary tumor urine specimens were utilized to generate bladder cancer organoids, which further treated with...
3D bioprinting is an emerging biofabrication strategy using bioinks, comprising cells and biocompatible materials, to produce functional tissue models. Despite progress in building increasingly complex objects, biological analyses printed constructs remain challenging. Especially, methods that allow non-invasive non-destructive evaluation of embedded are largely missing. Here, we implemented Raman imaging for molecular-sensitive investigations on bioprinted objects. Different aspects such as...
Electrospun collagen mats were analyzed using circular dichroism, enzymatic digestion, SDS-PAGE, western blotting, and Raman spectroscopy compared to heat-denaturated electrospun in HFIP.
The use of biomaterials and biomaterial functionalization is a promising approach to support pancreatic islet viability posttransplantation in an effort reduce insulin dependence for patients afflicted with diabetes mellitus type 1. Extracellular matrix (ECM) proteins are known impact numerous reparative functions the body. Assessing how endogenously expressed ECM affected by posttransplant-like hypoxic conditions may provide significant insights toward development tissue-engineered...
Two of the greatest challenges for successful application small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing inflammatory response to graft guide functional tissue regeneration. This study evaluates vivo performance electrospun resorbable elastomeric grafts, dual-functionalized with anti-thrombogenic heparin (hep) anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach. The regenerative capacity IL-4/hep, hep-only,...
Cardiomyopathies are associated with severe fibrotic remodeling of the heart, which is characterized by excessive accumulation collagen type I (COL I). In this study, we used marker-independent Raman microspectroscopy (RMS) to gain a deeper understanding disease’s underlying molecular and cellular mechanisms.
Cardiac hypertrophy is characterized by remodeling of the myocardium, which involves alterations in ECM (extracellular matrix) and cardiomyocyte structure. These critically contribute to impaired contractility relaxation, ultimately leading heart failure. Emerging evidence implicates that extracellular signaling molecules are involved pathogenesis cardiac remodeling. The immunophilin CyPA (cyclophilin A) has been identified as a potential culprit. In this study, we aimed unravel interplay...
Frozen cryopreservation (FC) with the vapour phase of liquid nitrogen storage (−135°C) is a standard biobank technique to preserve allogeneic heart valves enable preferable allograft valve replacement in clinical settings. However, their long-term function limited by immune responses, inflammation and structural degeneration. Ice-free cryopreserved (IFC) warmer possibilities at −80°C showed better matrix preservation decreased immunological response preliminary short-term vivo studies. Our...
Postoperative abdominal adhesions are responsible for serious clinical disorders. Administration of plasma-activated media (PAM) to cell type-specific modulated proliferation and protein biosynthesis is a promising therapeutic strategy prevent pathological responses in the context wound healing We analyzed PAM as option based on anti-adhesive responses. Primary human peritoneal fibroblasts mesothelial cells were isolated, characterized exposed different dosages. Cell effects components...