A5026685270- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Hormonal Regulation and Hypertension
- Cancer, Hypoxia, and Metabolism
- Adrenal and Paraganglionic Tumors
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Multicomponent Synthesis of Heterocycles
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Chemical Synthesis and Analysis
- Adrenal Hormones and Disorders
- Epigenetics and DNA Methylation
- Wastewater Treatment and Nitrogen Removal
- Glioma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Aquaculture Nutrition and Growth
- Synthesis of heterocyclic compounds
- Bacteriophages and microbial interactions
- Alzheimer's disease research and treatments
- Crustacean biology and ecology
- Concrete and Cement Materials Research
- Marine Toxins and Detection Methods
Shandong University
2015-2025
Quality Research
2025
Institut Français de la Mer
2025
Ocean University of China
2025
Qingdao National Laboratory for Marine Science and Technology
2025
Henan Forestry Vocational College
2025
Shandong Center for Disease Control and Prevention
2016-2025
Liaoning Shihua University
2021-2025
Ministry of Agriculture and Rural Affairs
2025
Pingjin Hospital
2018-2025
Glioblastomas are malignant tumors of the central nervous system hallmarked by subclonal diversity and dynamic adaptation amid developmental hierarchies. The source reorganization within spatial context these remains elusive. Here, we characterized glioblastomas spatially resolved transcriptomics, metabolomics, proteomics. By deciphering regionally shared transcriptional programs across patients, infer that glioblastoma is organized segregation lineage states adapts to inflammatory and/or...
Abstract We present the first analytical approach to demonstrate in situ imaging of metabolites from formalin‐fixed, paraffin‐embedded ( FFPE ) human tissue samples. Using high‐resolution matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry MALDI‐FT‐ICR MSI ), we conducted a proof‐of‐principle experiment comparing metabolite measurements and fresh frozen sections, found an overlap 72% amongst 1700 m/z species. In particular, observed...
Abstract Excess nutrient uptake and altered hormone secretion in the gut contribute to a systemic energy imbalance, which causes obesity an increased risk of type 2 diabetes colorectal cancer. This functional maladaptation is thought emerge at level intestinal stem cells (ISCs). However, it not clear how obesogenic diet affects ISC identity fate. Here we show that induces progenitor hyperproliferation, enhances differentiation cell turnover changes regional identities ISCs enterocytes mice....
Abstract Purpose: Current systems of gastric cancer molecular classification include genomic, molecular, and morphological features. Gastric based on tissue metabolomics remains lacking. This study aimed to define metabolically distinct subtypes identify their clinicopathological characteristics. Experimental Design: Spatial by high mass resolution imaging spectrometry was performed in 362 patients with cancer. K−means clustering used tumor stroma-related metabolites. The identified were...
For the first time, catalytic stability of cobalt metal, nitride, and phosphide phases supported on MgO was compared for dry reforming methane. The initial CH4 conversions Co/MgO, Co4N/MgO, Co2P/MgO catalysts were 88.1, 87.7 86.0%, respectively. However, after 15 h testing, these values gradually decreased to 69.9, 75.0 79.4%, Co2P catalyst demonstrated superior Co Co4N catalysts. enhanced attributed its smaller particle size a lower ratio CO2 dissociation rates.
An outbreak of Streptococcus suis serotype 2 emerged in the summer 2005 Sichuan Province, and sporadic infections occurred 4 additional provinces China. In total, 99 S. strains were isolated analyzed this study: 88 isolates from human patients 11 diseased pigs. We defined 98 as pulse type I by using pulsed-field gel electrophoresis analysis SmaI-digested chromosomal DNA. Furthermore, multilocus sequence typing classified 97 members same (ST), ST-7. Isolates ST-7 more toxic to peripheral...
Cryo-electron microscopy in conjunction with advanced image analysis was used to analyze the structure of 26S proteasome and elucidate its variable features. We have been able outline boundaries ATPase module "base" part regulatory complex that can vary position orientation relative 20S core particle. This variation is consistent "wobbling" model previously proposed explain role opening gate alpha-rings In addition, a mass near mouth ring has identified as Rpn10, multiubiquitin receptor, by...
Abstract Conditions of impaired adrenal function and tissue destruction, such as in Addison’s disease, treatment resistance adrenocortical carcinoma (ACC) necessitate improved understanding the pathophysiology cell death. Due to relevant oxidative processes cortex, our study investigated role ferroptosis, an iron-dependent death mechanism found high expression glutathione peroxidase 4 (GPX4) long-chain-fatty-acid CoA ligase (ACSL4) genes, key factors initiation ferroptosis. By applying MALDI...
Abstract Progressive respiratory failure and hyperinflammatory response is the primary cause of death in coronavirus disease 2019 (COVID-19) pandemic. Despite mounting evidence disruption hypothalamus-pituitary-adrenal axis COVID-19, relatively little known about tropism severe acute syndrome 2 (SARS-CoV-2) to adrenal glands associated changes. Here we demonstrate viral replication COVID-19 patients. Adrenal showed inflammation accompanied by inflammatory cell death. Histopathologic analysis...
The response to neoadjuvant chemotherapy (NAC) differs substantially among individual patients with non-small cell lung cancer (NSCLC). Major pathological (MPR) is a histomorphological read-out used assess treatment and prognosis in NSCLC after NAC. Although spatial metabolomics promising tool for evaluating metabolic phenotypes, it has not yet been utilized therapy responses NSCLC. We evaluated the potential application of tissues NAC, using classifier that utilizes mass spectrometry...
Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved tissue reorganization. However, contrast to genomic analysis, the actual biomolecular composition sample has fallen behind, leaving a gap potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable filling this gap. In study we demonstrate spatially...
Abstract Background Trastuzumab is the only first-line treatment targeted against human epidermal growth factor receptor 2 (HER2) approved for patients with HER2-positive advanced gastric cancer. The impact of metabolic heterogeneity on trastuzumab efficacy remains unclear. Methods Spatial metabolomics via high mass resolution imaging spectrometry was performed in pretherapeutic biopsies cancer a prospective multicentre observational study. spectra, representing within tumour areas, were...
Primary aldosteronism is frequently caused by an adrenocortical aldosterone-producing adenoma (APA) carrying a somatic mutation that drives aldosterone overproduction. APAs with in KCNJ5 (APA-KCNJ5MUT) are characterized heterogeneous CYP11B2 (aldosterone synthase) expression, particular cellular composition and larger tumor diameter than those wild-type (APA-KCNJ5WT). We exploited these differences to decipher the roles of transcriptome metabolome reprogramming pathogenesis. Consecutive...
In the adrenal gland, neuroendocrine cells that synthesize catecholamines and epithelial produce steroid hormones are united beneath a common organ capsule to function as single stress-responsive organ. The functional anatomy of hormone–producing cortex catecholamine-producing medulla is ill defined at level small molecules. Here, we report comprehensive high-resolution mass spectrometry imaging (MSI) map normal human gland. A large variety biomolecules was accessible by matrix-assisted...
MS imaging (MSI) is a valuable tool for diagnostics and systems biology studies, being highly sensitive, label-free technique capable of providing comprehensive spatial distribution different classes biomolecules. The application MSI to the study endogenous compounds has received considerable attention because metabolites are result interactions biosystem with its environment. can therefore enhance understanding disease mechanisms elucidate biological variation. We present in situ...
Recent genetic examinations and multisteroid profiles have provided the basis for subclassification of aldosterone-producing adenomas (APAs). The objective current study was to produce a comprehensive, high-resolution mass spectrometry imaging (MSI) map APAs in relation morphometry, immunohistochemical profiles, mutational status, clinical outcome. cohort comprised 136 patients with unilateral primary aldosteronism. Matrix-assisted laser desorption/ionization–Fourier transform–ion cyclotron...
Aldosterone-producing adenomas (APAs) are one of the main causes primary aldosteronism and most prevalent surgically correctable form hypertension. cell clusters (APCCs) comprise tight nests zona glomerulosa cells, strongly positive for CYP11B2 (aldosterone synthase) in immunohistochemistry. APCCs have been suggested as possible precursors APAs because they frequently carry driver mutations constitutive aldosterone production, a few adrenal lesions with histopathologic features both...
Diabetic foot is one of the main causes non-traumatic amputation. However, there still lack effective drugs to treat diabetic in clinical practice. Kanglexin (KLX) a new anthraquinone compound with cardiovascular protective effects. Here we report that KLX accelerates wound healing by promoting angiogenesis via FGFR1/ERK signaling. Firstly, KM mice were injected (ip) streptozocin establish type 1 model. The full thickness diameter 5 mm was prepared on back each mice. wounds treated once day...