A5003285023- Signaling Pathways in Disease
- Cardiac Arrhythmias and Treatments
- Atrial Fibrillation Management and Outcomes
- Cardiac pacing and defibrillation studies
- Cell Adhesion Molecules Research
- Cardiac electrophysiology and arrhythmias
- Cardiac Fibrosis and Remodeling
- Viral Infections and Immunology Research
- Cardiomyopathy and Myosin Studies
- Proteoglycans and glycosaminoglycans research
- Acute Myocardial Infarction Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Spectroscopy and Chemometric Analyses
- Cardiac Structural Anomalies and Repair
- Glycosylation and Glycoproteins Research
- Platelet Disorders and Treatments
- Cardiovascular Function and Risk Factors
- Blood Coagulation and Thrombosis Mechanisms
- Mitochondrial Function and Pathology
- Labor Movements and Unions
- Cardiac Arrest and Resuscitation
- Pancreatic function and diabetes
- Antiplatelet Therapy and Cardiovascular Diseases
University Children's Hospital Tübingen
2017-2025
University of Tübingen
2015-2024
Universitätsklinikum Tübingen
2015-2024
Cleveland Clinic
2015
Martin Luther University Halle-Wittenberg
2014
// Xi Feng 1 , Frank Szulzewsky 2,* Alexan Yerevanian 1,3,* Zhihong Chen David Heinzmann 1,4 Rikke Darling Rasmussen Virginia Alvarez-Garcia Yeonghwan Kim 5 Bingcheng Wang 6 Ilaria Tamagno Hao Zhou 7 Xiaoxia Li Helmut Kettenmann 2 Richard M. Ransohoff 1,8 and Dolores Hambardzumyan Department of Neurosciences at Cleveland Clinic, Cleveland, Ohio, USA Cellular Neurosciences, Max Delbrück Center for Molecular Medicine, Berlin, Germany 3 Case Western Reserve University School 4 Cardiology...
Abstract Background COVID-19 infection may cause severe respiratory distress and is associated with increased morbidity mortality. Impaired cardiac function and/or pre-existing cardiovascular disease be poor prognosis. In the present study, we report a comprehensive characterization in first consecutive collective of patients that was admitted treated at University Hospital Tübingen, Germany. Methods 123 were included. Routine blood sampling, transthoracic echocardiography...
Cyclophilin A (CyPA) is secreted under inflammatory conditions by various cell types. Whereas the important role of intracellular CyPA for platelet function has been reported, effect extracellular on not investigated yet.Inhibition through a novel specific inhibitor MM284 reduced thrombus after ferric chloride-induced injury in vivo. In vitro enhanced formation even CyPA(-/-) platelets. Treatment isolated platelets with recombinant resulted degranulation time- and dose-dependent manner....
Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved tissue reorganization. However, contrast to genomic analysis, the actual biomolecular composition sample has fallen behind, leaving a gap potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable filling this gap. In study we demonstrate spatially...
Focal pulsed field ablation (PFA) is a novel technique for treating cardiac arrhythmias. It has demonstrated positive results in initial studies and good safety profile. In recent studies, PFA was often utilized first-time pulmonary vein isolation (PVI) performed under general anesthesia. our study, we assessed the feasibility, safety, acute procedural efficacy, efficiency of focal deep sedation patients, 80% whom had undergone at least one left atrial previously. We treated 30 patients (71...
Background: Atrial fibrosis is a hallmark of arrhythmogenic structural remodeling in patients with persistent atrial fibrillation (AF) and negatively correlated procedure outcome undergoing ablation. However, noninvasive methods to determine the extent are limited. Here, we used microRNA (miRNA) expression analysis detect markers left low-voltage areas (LVAs) AF catheter Methods: We performed 3-dimensional voltage mapping 102 (average age 62.1±13.1 years, CHA 2 DS -VASc score 2.3±1.6, LA...
Abstract Platelet activation plays a critical role in thrombosis. Inhibition of platelet is cornerstone treatment acute organ ischemia. ACKR3 surface expression independently associated with all-cause mortality CAD patients. In novel genetic mouse strain, we show that megakaryocyte/platelet-specific deletion results enhanced and thrombosis vitro vivo. Further, performed ischemia/reperfusion experiments (transient LAD-ligation tMCAO) mice to assess the impact deficiency platelets on tissue...
Cyclophilins are a group of highly conserved cytosolic enzymes that have peptidylprolyl cis/trans isomerase activity. Cyclophilin A (CyPA) can be secreted in the extracellular space by inflammatory cells and upon cell death. The presence CyPA patients with non-ischemic cardiomyopathy is associated poor clinical prognosis. Here, we investigated inhibition mouse model troponin I-induced autoimmune myocarditis using strictly CyPA-inhibitor MM284. Since A/J mice develop severe inflammation...
Cyclophilin A (CyPA) induces leucocyte recruitment and platelet activation upon release into the extracellular space. Extracellular CyPA therefore plays a critical role in immuno-inflammatory responses tissue injury thrombosis activation. To date, CD147 (EMMPRIN) has been described as primary receptor mediating effects of platelets leucocytes. The for advanced glycation end products (RAGE) shares inflammatory prothrombotic properties also found to have similar ligands CD147. In this study,...
Objective: Recently, we reported that extracellular cyclophilin A (CyPA) is an important agonist for platelets. Whereas soluble CyPA-levels have been associated with cardiovascular risk factors, cell-bound CyPA has not investigated yet. In this study, analyzed the first time platelet-bound in patients symptomatic coronary artery disease (CAD). Methods and results: blood was obtained from 388 consecutive patients: 204 stable CAD 184 acute syndrome (76 unstable angina, 78 non ST-elevation...
Surface receptor-mediated adhesion is a fundamental step in the recruitment of leukocytes and platelets, as well platelet–leukocyte interactions. The surface receptor CD147 crucially involved host defense against self-derived invading targets, thrombosis. In current study, we describe previously unknown interaction with integrin αMβ2 (Mac-1) this context. Using binding assays, were able to show stable Mac-1 vitro. Leukocytes from Mac-1−/− CD147+/− mice showed markedly reduced static CD147-...
Abstract Cyclophilin A (CyPA) is involved in the pathophysiology of several inflammatory and cardiovascular diseases. To our knowledge, there no specific inhibitor targeting extracellular CyPA without affecting other cyclophilins or intracellular functions. In this study, we developed an antibody-based analysed its effects vitro vivo. generate a antibody, mice rats were immunized with peptide containing matrix metalloproteinase inducer binding site various antibody clones selected purified....
Abstract During megakaryopoiesis, megakaryocytes (MKs) undergo cellular morphological changes with strong modification of membrane composition and lipid signaling. Here, we adopt a lipid-centric multiomics approach to create quantitative map the MK lipidome during maturation proplatelet formation. Data reveal that differentiation is driven by an increased fatty acyl import de novo synthesis, resulting in anionic phenotype. Pharmacological perturbation acid phospholipid synthesis blocked...
Cardiac hypertrophy is characterized by remodeling of the myocardium, which involves alterations in ECM (extracellular matrix) and cardiomyocyte structure. These critically contribute to impaired contractility relaxation, ultimately leading heart failure. Emerging evidence implicates that extracellular signaling molecules are involved pathogenesis cardiac remodeling. The immunophilin CyPA (cyclophilin A) has been identified as a potential culprit. In this study, we aimed unravel interplay...
Background/Aims: Fibrotic remodeling of the atria plays a key role in pathogenesis atrial fibrillation (AF). As little is known about contribution circulating monocytes and pathophysiology AF, we investigated profibrotic factors different subsets obtained from patients with undergoing catheter ablation. Methods: A 3D high density voltage mapping was performed sinus rhythm to evaluate extent low-voltage areas (LVAs) 71 persistent AF. Low-voltage defined as signals < 0.5mV during rhythm....
The role of cryoballoon (CB) pulmonary vein isolation (PVI) for patients with persistent atrial fibrillation (AF) is controversial, since long-term success can be poor. We performed left voltage mapping before CB PVI and determined AF-free survival depending on the extent low-voltage areas (LVAs).We consecutively enrolled 60 AF (average age, 60.6 ± 12.9 years; CHA2 DS 2 VASc score, 2.3 1.6; size 46.0 5.2 mm) who were planned PVI. Before ablation, we (Abbott EnSite Precision or Velocity)....
Risk stratification of patients with non-ischemic dilated cardiomyopathy remains a matter debate in the era device implantation.
COVID-19 may lead to severe acute respiratory distress syndrome (ARDS) resulting in increased morbidity and mortality. Heart failure and/or pre-existing cardiovascular disease correlate with poor outcomes thus require special attention from treating physicians. The present study sought investigate a possible impact of impaired myocardial function as well markers on mortality or ARDS need for mechanical ventilation 157 consecutive patients confirmed SARS-CoV-2 infection. All were admitted...
Impairment of renal phosphate elimination in chronic kidney disease (CKD) leads to enhanced plasma and tissue concentration, which turn up-regulates transcription factor NFAT5 serum & glucocorticoid-inducible kinase SGK1. The upregulates ORAI1, a Ca2+-channel accomplishing store-operated Ca2+-entry (SOCE). ORAI1 is stimulated following intracellular store depletion by Ca2+-sensors STIM1 and/or STIM2. In megakaryocytes blood platelets SOCE thus are powerful regulators activity. present study...