A5040820485- Plasma Applications and Diagnostics
- Heat shock proteins research
- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Circular RNAs in diseases
- Plasma Diagnostics and Applications
- Endoplasmic Reticulum Stress and Disease
- Nanoplatforms for cancer theranostics
- Cancer Immunotherapy and Biomarkers
- Burn Injury Management and Outcomes
- Hormonal and reproductive studies
- Protein Kinase Regulation and GTPase Signaling
- Bladder and Urothelial Cancer Treatments
- Cancer Cells and Metastasis
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Surface Modification and Superhydrophobicity
- Plasma and Flow Control in Aerodynamics
- Wound Healing and Treatments
University Hospital Bonn
2020-2025
University of Bonn
2020-2022
Klinik und Poliklinik für Urologie, Kinderurologie und Andrologie
2021
University Hospital Münster
2021
Charité - Universitätsmedizin Berlin
2021
Ruhrlandklinik
2021
West German Heart and Vascular Center Essen
2021
Institut für Medizinische Informatik, Biometrie und Epidemiologie
2021
Universitätsmedizin Greifswald
2013-2020
Deutsche Gesellschaft für Urologie
2020
One of the promising possibilities clinical application cold plasma, so-called atmospheric plasma (CAP), is its on malignant cells and cancer tissue using anti-neoplastic effects, primarily through delivery reactive oxygen nitrogen species (ROS, RNS). In this study, we investigated impact CAP cellular proliferation consecutive molecular response mechanisms in established prostate (PC) cell lines. PC showed a significantly reduced growth following treatment as result both an immediate...
Objective So-called cold physical plasmas for biomedical applications generate reactive oxygen and nitrogen species the latter can trigger DNA damage at high concentrations. Therefore, mutagenic risks of a certified atmospheric pressure argon plasma jet (kINPen MED) its predecessor model 09) were assessed. Methods Inner egg membranes fertilized chicken eggs received single treatment with either kINPen 09 (1.5, 2.0, or 2.5 min) MED (3, 4, 5, 10 min). After three days incubation, blood smears...
Apoptosis results in specific and stage-dependent morphological alterations of the cell nucleus, including pyknosis shrinking. The experimental investigation apoptotic processes is still challenging routinely based on assessment molecular events like chromatin fragmentation caspase enzyme activity. Alternatively, establishment a fluorescence microscopy nuclear morphology assay would provide simple robust low-cost method for detection quantification cascades.Model lines LNCaP MDA-MB-231 were...
(1) Background: Cervical intraepithelial neoplasia (CIN) of long-term persistence or associated with individual treatment indications often requires highly invasive treatments. These are risks bleeding, infertility, and pregnancy complications. For low- middle-income countries (LMICs), standard procedures difficult to implement manage. We characterized the application energized gas "noninvasive physical plasma" (NIPP) for tissue devitalization CIN. (2) Methods: report establishment a...
We have recently reported that two typical Gs-coupled receptors, the beta2-adrenergic receptor and for prostaglandin E1, stimulate phospholipase C-epsilon (PLC-epsilon) increase intracellular Ca2+ concentration ([Ca2+]i) in HEK-293 cells N1E-115 neuroblastoma cells, respectively, by a pathway involving Epac1, cAMP-activated Rap-specific guanine nucleotide exchange factor (GEF), GTPase Rap2B. Here we demonstrated these receptors use this to activate H-Ras extracellular signal-regulated...
Antiestrogens are universally used to treat estrogen receptor--positive breast cancer, but relapses occur commonly due the development of drug resistance. The ability antiestrogen induce transforming growth factor beta (TGFbeta) in cancer cells may be relevant emergence resistance, not only at level cell autonomous effects TGFbeta on progression also its host immune system. To evaluate potential role tumor-derived, antiestrogen-induced as an suppressor, we established vitro mixed lymphocyte...
Ovarian cancer (OC) is a gynecologic tumor with poor prognosis. Despite radical cytoreductive surgery and platinum-based adjuvant systemic treatment, OC will relapse in the majority of cases. Thus, cold atmospheric plasma (CAP), highly reactive physical state bearing diverse biological activities being suited for anticancer therapy, may be promising option therapy.OC cell lines were exposed either directly to CAP or culture medium previously CAP. Cell proliferation motility was measured.The...
Cold physical plasmas ignited a technological spark in industry, biotechnology, and medicine. Especially the field of hygiene benefited plasma's exceptional activity against pathogenic microorganisms. Together with plasma‐based surface functionalization, these qualities are highly relevant variety processes health care, such as decontamination or sterilization medical devices, food, packaging materials, waste water, indoor air. In medicine, plasma has proven to show promising antiseptic...
Phosphorylated histone 2AX ( γ H2AX) is a long-standing marker for DNA double-strand breaks (DSBs) from ionizing radiation in the field of radiobiology. This led to perception H2AX being general direct damage with treatment other agents such as low-dose exogenous ROS that unlikely act on cellular directly. Cold physical plasma confers biomedical effects majorly via release reactive oxygen and nitrogen species (ROS). In vitro , increase has often been observed treatment, leading conclusion...
Abstract Non-invasive physical plasma (NIPP) has been used effectively for wound healing in human medicine over two decades. The advantages are that NIPP few side effects, is painless and gentle on the tissue. therapeutic effect mediated by reactive oxygen species (ROS). Based biomedical effects known to date, it can be assumed also treatment non-human mammals. In this prospective, non-randomized monocentric clinical trial, 43 European hedgehogs with cut bite wounds were treated conventional...
Abstract Background Radiation dermatitis (RD) is the most common side effect of breast irradiation, yet only few potent preventative and therapeutic options are available. Following encouraging results from a phase 1 2 trial on topical use non-invasive physical plasma (NIPP), very well-tolerated treatment option to promote tissue regeneration generated ambient air, we now present study protocol for planned 3 trial. Methods In this randomised double-blind placebo-controlled trial, patients...
Cold atmospheric plasma has been shown to inhibit tumor cell growth and induce death. The aim of the study was investigate effects cold treatment on proliferation human osteosarcoma cells characterize underlying cellular mechanisms.Human (U2-OS MNNG/HOS) were treated with seeded in culture plates. Cell proliferation, p53 phospho-p53 protein expression nuclear morphology assessed.The lines exhibited attenuated rates by up 66%. revealed an induction p53, as well expression, 2.3-fold 4.5-fold,...
Nonthermal treatment with cold atmospheric plasma (CAP) is a promising option for local of chronic-inflammatory and precancerous lesions as well various mucosal cancer diseases, besides its primary indication wound healing antiseptics. Atmospheric pressure jets (APPJs) are versatile sources, some which well-characterized medically approved. The characterization APPJs, however, often based on the simple solutions or even studies effluent itself. To better assess in vivo effects CAP treatment,...
Abiraterone provides significant survival advantages in prostate cancer (PC), however, the current understanding of molecular mechanisms abiraterone is still limited. Therefore, impact on androgen receptor (AR)-positive LNCaP and AR-negative PC-3 cells was assessed by cellular analyses. The present study demonstrated, that treatment significantly decreased cell growth, AR expression, activity AR-positive cells. Notably, exhibited comparable reductions proliferation, associated with DNA...