A5100784707- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Mycotoxins in Agriculture and Food
- Fungal Infections and Studies
- Streptococcal Infections and Treatments
- Infectious Diseases and Mycology
- MicroRNA in disease regulation
- Neonatal and Maternal Infections
- Bone Metabolism and Diseases
- Reproductive System and Pregnancy
- Cellular Mechanics and Interactions
- Animal Virus Infections Studies
- Enterobacteriaceae and Cronobacter Research
- Fungal and yeast genetics research
- Inflammation biomarkers and pathways
- Sirtuins and Resveratrol in Medicine
- Microbial Metabolic Engineering and Bioproduction
- Protein Kinase Regulation and GTPase Signaling
- Estrogen and related hormone effects
- Infection Control in Healthcare
- Antimicrobial Resistance in Staphylococcus
- Virus-based gene therapy research
- Ion channel regulation and function
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Anhui Medical University
2024
Hunan Agricultural University
2024
Huazhong Agricultural University
2013-2024
Guangxi University
2024
Guangxi Veterinary Research Institute
2024
Guangxi Center for Disease Prevention and Control
2024
State Key Laboratory of Agricultural Microbiology
2024
Southwest Jiaotong University
2024
Beijing University of Chinese Medicine
2022-2024
Shanghai Jiao Tong University
2024
Mechanical loading, such as caused by exercise, stimulates bone formation osteoblasts and increases strength, but the mechanisms are poorly understood. Osteocytes reside in matrix, sense changes mechanical load, produce signals that alter osteoblasts. We report ion channel Piezo1 is required for gene expression induced fluid shear stress cultured osteocytes stimulation of a small molecule agonist sufficient to replicate effects flow on osteocytes. Conditional deletion notably reduced mass...
The detection of estrogen receptor-α (ERα) in osteoblasts and osteoclasts over 20 years ago suggested that direct effects estrogens on both these cell types are responsible for their beneficial the skeleton, but role ERα osteoblast lineage cells has remained elusive. In addition, activation can only account protective effect cancellous, not cortical, bone compartment represents 80% entire skeleton. Here, we deleted at different stages differentiation murine cells. We found progenitors...
Summary Age‐related bone loss in mice results from a decrease formation and an increase cortical resorption. The former is accounted by the number of postmitotic osteoblasts which synthesize matrix thought to be consequence age‐dependent changes mesenchymal osteoblast progenitors. However, there are no specific markers for these progenitors, conclusions rely on vitro cultures mixed cell populations. Moreover, culprits such remain unknown. Here, we have used Osx1‐Cre;Td RFP osteoprogenitors...
A decline of the levels and activity Sirtuin1 (Sirt1), a NAD+ class III histone deacetylase, with age contributes to development several diseases including type 2 diabetes, neurodegeneration, inflammation, cancer. The anti-aging effects Sirt1 evidently result from deacetylation many transcription factors co-factors members Forkhead box O (FoxO) family β-catenin. Wnt/β-catenin is indispensable for osteoblast generation. FoxOs, on other hand, sequester β-catenin inhibit osteoprogenitor...
Infection with the Streptococcus suis (S. suis) epidemic strain can cause Streptococcal toxic shock-like syndrome (STSLS), which is characterized by a cytokine storm, dysfunction of multiple organs and high incidence mortality despite adequate treatment. Despite some progress concerning contribution inflammatory response to STSLS, precise mechanism underlying STSLS development remains elusive. Here, we use murine model demonstrate that caspase-1 activity critical for development....
ABSTRACT Old age and sex steroid deficiency are the two most critical factors for development of osteoporosis. It remains unknown, however, whether molecular culprits conditions similar or distinct. We show herein that at 19.5 months age—a time by which age-dependent decline cortical cancellous bone mass porosity were fully manifested in C57BL/6J mice—these animals remained functionally estrogen sufficient. Transgenic mice with conditional expression mitochondria-targeted catalase—a potent...
The protein folding capacity of the endoplasmic reticulum (ER) is tightly regulated by a network signaling pathways, known as unfolded response (UPR). UPR sensors monitor ER status to adjust according need. To understand how sensor IRE1 maintains homeostasis, we identified zero-length crosslinks RNA with single nucleotide precision in vivo. We found that specifically subset ER-targeted mRNAs, SRP RNA, ribosomal and transfer RNAs. Crosslink sites cluster discrete region ribosome surface...
Activation of Sirtuin1 (Sirt1), an nicotinamide adenine dinucleotide oxidized-dependent deacetylase, by natural or synthetic compounds like resveratrol, SRT2104, SRT3025 attenuates the loss bone mass caused ovariectomy, aging, unloading in mice. Conversely, Sirt1 deletion osteoclast progenitors increases number and resorption. deacetylates forkhead box protein (Fox) O1, FoxO3, FoxO4, thereby modulates their activity. FoxOs restrain osteoclastogenesis Here, we tested hypothesis that...
Because of recent insights into the pathogenesis age-related bone loss, we investigated whether intermittent parathyroid hormone (PTH) administration antagonizes molecular mechanisms adverse effects aging on bone. Parathyroid produced a greater increase in vertebral trabecular mineral density and volume as well expansion endocortical surface femur 26- when compared to 6 -month-old female C57BL/6 mice. Moreover, PTH increased connectivity vertebrae, toughness both vertebrae femora old, but...
ABSTRACT In men, androgens are critical for the acquisition and maintenance of bone mass in both cortical cancellous compartment. Male mice with targeted deletion androgen receptor (AR) mature osteoblasts or osteocytes have lower mass, but no phenotype. We investigated possibility that effects on compartment result from AR signaling osteoprogenitors cells osteoclast lineage; via estrogen alpha (ERα) either these two cell types upon conversion testosterone to estradiol. To this end, we...
Azole resistance in Aspergillus fumigatus has emerged as a worldwide public health problem. We sought here to demonstrate the occurrence and characteristics of azole A. from different parts China. A total 317 clinical 144 environmental isolates 12 provinces were collected subjected screening for resistance. Antifungal susceptibility, cyp51A gene sequencing, genotyping carried out all suspected azole-resistant subset azole-susceptible isolates. As result, 8 (2.5%) 2 (1.4%) identified...
Abstract Both an increase in osteoclast and a decrease osteoblast numbers contribute to skeletal aging. Markers of cellular senescence, including expression the cyclin inhibitor p16, with aging several bone cell populations. The elimination p16‐expressing cells old mice, using INK‐ATTAC transgene, increases mass indicating that senescent However, identity extent which ablation may prevent remain unknown. Using mice expressing p16‐3MR we examined whether between 12 24 months age could...
Dendrobium officinale Kimura et Migo is a commercially and pharmacologically highly prized species widely used in Western Asian countries. In contrast to the extensive genomic transcriptomic resources generated this medicinal species, detailed metabolomic data are still missing. Herein, using targeted metabolomics approach, we detect 649 diverse metabolites leaf stem samples of D. officinale. The majority these were organic acids, amino acids their derivatives, nucleotides flavones. Though...
Because sex steroids regulate the life span of bone cells by modulating cytoplasmic kinase activity via a nongenotropic action their classical receptors, we have explored possibility that vitamin D nuclear receptor (VDR) might exhibit similar actions. We report conformationally flexible full VDR agonist, 1α,25(OH)2-vitamin D3 (1α,25(OH)2D3), and 6-s-cis-locked 1α,25(OH)2-lumisterol3 (JN) analog, also acting through but with poor transcriptional activity, protected murine osteoblastic or...
Lung epithelial cells constitute the first defense line of host against inhaled Aspergillus fumigatus; however, transcriptional response human alveolar type II was still unclear. Here we used RNA-Seq technology to assess transcriptome profiles A549 following direct interaction with conidia A. fumigatus. The total number identified genes 19118. Compared uninfected cells, 459 were differentially expressed in co-incubated for 8 h, including 302 up-regulated and 157 down-regulated genes. GO KEGG...
In 2018, we conducted a cross-sectional study to investigate azole resistance in environmental Aspergillus fumigatus isolates obtained from different agricultural fields China. Using 63 soil cores, cultured for azole-resistant A. and characterized by their cyp51A gene type, short tandem repeat genotype, mating type. Of 206 isolates, 21 (10.2%) were resistant. Nineteen of had mutations (5 TR34/L98H, 8 TR34/L98H/S297T/F495I, 6 TR46/Y121F/T289A). Eighteen samples acquired strawberry fields,...