A5000727397- Bone Metabolism and Diseases
- Birth, Development, and Health
- Bone health and osteoporosis research
- Phytoestrogen effects and research
- Estrogen and related hormone effects
- Bone health and treatments
- Multiple Myeloma Research and Treatments
- Fatty Acid Research and Health
- Adipose Tissue and Metabolism
- Inflammatory mediators and NSAID effects
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gestational Diabetes Research and Management
- Nutrition, Genetics, and Disease
- Peptidase Inhibition and Analysis
- Telomeres, Telomerase, and Senescence
- Vitamin D Research Studies
- Pregnancy and preeclampsia studies
- Phytochemicals and Antioxidant Activities
- NF-κB Signaling Pathways
- Infant Nutrition and Health
- Cytokine Signaling Pathways and Interactions
- GDF15 and Related Biomarkers
- CAR-T cell therapy research
University of Arkansas for Medical Sciences
2016-2025
Arkansas Children's Nutrition Center
2016-2025
University of Arkansas at Fayetteville
2009-2024
Winthrop Rockefeller Foundation
2024
Arkansas Department of Agriculture
2010-2021
Louisiana State University Health Sciences Center New Orleans
2018
Texas A&M University
2018
California State University, Long Beach
2012
Pediatrics and Genetics
2011
Central Arkansas Veterans Healthcare System
2002-2004
Glucocorticoids depress bone formation by inhibiting osteoblastogenesis and increasing osteoblast apoptosis. However, the role of resorption in initial rapid phase loss characteristic glucocorticoid-induced osteoporosis is unexplained, reason for efficacy bisphosphonates this condition remains unknown. We report that murine osteoclast cultures, glucocorticoids prolonged baseline survival osteoclasts antagonized bisphosphonate-induced caspase activation apoptosis a glucocorticoid...
Glucocorticoids depress bone formation by inhibiting osteoblastogenesis and increasing osteoblast apoptosis. However, the role of resorption in initial rapid phase loss characteristic glucocorticoid-induced osteoporosis is unexplained, reason for efficacy bisphosphonates this condition remains unknown. We report that murine osteoclast cultures, glucocorticoids prolonged baseline survival osteoclasts antagonized bisphosphonate-induced caspase activation apoptosis a glucocorticoid...
It has been found that 4-estren-3alpha,17beta-diol, a synthetic ligand for the estrogen receptor (ER) or androgen (AR), which does not affect classical transcription, reverses bone loss in ovariectomized females orchidectomized males without affecting uterus seminal vesicles, demonstrating genotropic actions of sex steroid receptors are dispensable their bone-protective effects, but indispensable effects on reproductive organs. We have now investigated mechanism action this compound. report...
It has been found that 4-estren-3α,17β-diol, a synthetic ligand for the estrogen receptor (ER) or androgen (AR), which does not affect classical transcription, reverses bone loss in ovariectomized females orchidectomized males without affecting uterus seminal vesicles, demonstrating genotropic actions of sex steroid receptors are dispensable their bone-protective effects, but indispensable effects on reproductive organs. We have now investigated mechanism action this compound. report that,...
Diet and nutritional status are critical factors that influences bone development. In this report we demonstrate a mixture of phenolic acids found in the serum young rats fed blueberries (BB) significantly stimulated osteoblast differentiation, resulting increased mass. Greater formation BB diet-fed animals was associated with increases progenitors differentiation reduced osteoclastogenesis. Blockade p38 phosphorylation eliminated effects on activation Wnt signaling preosteoblasts. Knocking...
Maternal obesity at conception increases the risk of offspring obesity, thus propagating an intergenerational vicious cycle. Male born to obese dams are hyperresponsive high fat-diets, gaining greater body weight, fat mass, and additional metabolic sequelae compared lean controls. In this report, we identify impact maternal before conception, on embryo, intrauterine milieu during periimplantation period. We conducted global transcriptomic profiling in uterus blastocyst, gene/protein...
Background It is well established that excessive consumption of a high fat diet (HFD) results in obesity; however, the consequences obesity on postnatal skeletal development have not been studied. Methodology and Principal Findings Total enteral nutrition (TEN) was used to feed day 27 male rats intragastrically with 45% for four weeks induce obesity. Fat mass increased compared fed TEN diets containing 25% (medium diet, MFD) or chow (low LFD) ad libitum matched body weight gains. Serum...
The mechanisms by which chronic ethanol intake induces bone loss remain unclear. In females, the skeletal response to varies depending on physiologic status (e.g., cycling, pregnancy, or lactation). Ethanol-induced oxidative stress appears be a key event leading toxicity. this study, ethanol-containing liquid diets were fed postlactational female Sprague-Dawley rats intragastrically for 4 weeks beginning at weaning. Ethanol consumption decreased mineral density (BMD) compared with control...
Because sex steroids regulate the life span of bone cells by modulating cytoplasmic kinase activity via a nongenotropic action their classical receptors, we have explored possibility that vitamin D nuclear receptor (VDR) might exhibit similar actions. We report conformationally flexible full VDR agonist, 1α,25(OH)2-vitamin D3 (1α,25(OH)2D3), and 6-s-cis-locked 1α,25(OH)2-lumisterol3 (JN) analog, also acting through but with poor transcriptional activity, protected murine osteoblastic or...
Bone loss occurs following chronic ethanol (EtOH) consumption in males and cycling females part as a result of increased bone resorption. We have demonstrated vivo that estradiol treatment can reverse this effect. Using osteoclast precursors from marrow osteoblast/preosteoclast coculture, we found EtOH-induced receptor activator nuclear factor-κB ligand (RANKL) expression osteoblasts was able to promote osteoclastogenesis. These effects were blocked by pretreatment cells with either...
Epidemiological and animal studies have suggested that chronic alcohol consumption is a major risk factor for osteoporosis. Using bone from cycling female rats infused chronically with ethanol (EtOH) in vivo osteoblastic cells vitro, we found EtOH significantly increased estrogen receptor alpha (ERalpha) beta (ERbeta) mRNA ERalpha protein levels. Treatment 17beta-estradiol (E2) vitro interfered these effects of on cells. agonist propylpyrazoletriol (PPT) ERbeta diarylpropionitrile (DPN)...
Background Appropriate nutrition during early development is essential for maximal bone mass accretion; however, linkage between nutrition, childhood mass, peak in adulthood, and prevention of loss later life has not been studied. Methodology Principal Findings In this report, we show that feeding a high quality diet supplemented with blueberries (BB) to pre-pubertal rats throughout or only postnatal day 20 (PND20) PND34 prevented ovariectomy (OVX)-induced adult life. This protective effect...
Multiple myeloma (MM) growth is supported by an immune-tolerant bone marrow microenvironment. Here, we find that loss of Never in mitosis gene A (NIMA)-related kinase 2 (NEK2) tumor microenvironmental cells associated with MM suppression. The absence NEK2 leads to both fewer tumor-associated macrophages (TAMs) and inhibitory T cells. expression myeloid progenitor promotes the generation functional TAMs when stimulated conditional medium. Clinically, high increased CD8+ effector memory cells,...
Multiple myeloma (MM) is a malignancy of terminally differentiated B-cells that localized primarily in the bone marrow (BM) but also can be present peripheral blood and tissue/organs [...].
<title>Abstract</title> Blueberry metabolite-derived phenolic acids are thought to suppress bone resorption via interactions with the G protein-coupled receptor 109A (GPR109A). Previously, global GPR109A knockout (GPR109A<sup>⁻/⁻</sup>) mice exhibited increased mass and a diminished bone-protective response acids. While is highly expressed in osteoclast precursor macrophages, its role development remains unclear. To address this, we generated myeloid cell-specific...
Epidemiological studies show that maternal obesity during intrauterine and early postnatal life increases the risk of low bone mass fracture later in life. Here, we development is inhibited gestational embryonic day 18.5 (E18.5) embryos from rat dams made obese by feeding a high-fat diet (HFD). Moreover, fetal osteogenic calvarial cells (FOCCs) these have significantly less potential to develop into mature osteoblasts compared AIN-93G diet-fed controls. Profiling transcriptional genes for...
Previous in vitro data suggest that ethanol (EtOH) activates NADPH oxidase (Nox) osteoblasts leading to accumulation of reactive oxygen species (ROS). This might be a mechanism underlying inhibition bone formation and increased resorption observed vivo after EtOH exposure. In rat model which cycling females were infused intragastrically with EtOH-containing liquid diets, significantly decreased stimulated osteoblast-dependent osteoclast differentiation. These effects reversed by exogenous...
Nutritional status during intrauterine and early postnatal life impacts the risk of chronic diseases, presumably via epigenetic mechanisms. However, evidence on impact gestational events regulation embryonic bone cell fate is sparse. We investigated effects maternal obesity fetal osteoblast development in both rodents humans. Female rats were fed control or an obesogenic high-fat diet (HFD) for 12 weeks mated with male diets, respective diets continued pregnancy. Embryonic rat osteogenic...
Bone loss resulting from chronic ethanol (EtOH) abuse is frequently accompanied by altered vitamin D3 homeostasis. In the current study, we examined EtOH effects in a female rat model which control or EtOH-containing diets were infused intragastrically. treatment reduced plasma 1,25-dihydroxycholecalciferol (1,25 (OH)2 D3) coincident with decrease renal CYP27B1 (25(OH)D3 1α-hydroxylase) mRNA and an increase expression of CYP24A1 D3- 24-hydroxylase). induction occurred as result increased...
To investigate the effects of sex hormones on ethanol (EtOH)-induced bone loss, female Sprague-Dawley rats were fed control or EtOH-containing diets (12 g/kg/day) by intragastric infusion. After 3 weeks, receiving EtOH had significant decreases in tibial trabecular and total mineral density, induction receptor activator nuclear factor-κB ligand (RANKL) mRNA expression, enhanced resorption, all which prevented treatment with 17β-estradiol (E<sub>2</sub>). The addition progesterone did not...
Chronic alcohol abuse results in decreased bone mineral density (BMD), which can lead to increased fracture risk. In contrast, low levels of have been associated with BMD epidemiological studies. Alcohol9s toxic skeletal effects suggested involve impaired vitamin D/calcium homeostasis. Therefore, dietary D supplementation may be beneficial reducing loss chronic consumption. Six-week-old female C57BL/6J mice were pair-fed ethanol (EtOH)-containing liquid diets (10 or 36% total calories) for...
Previous studies have demonstrated that weanling rats fed AIN-93G semi-purified diets supplemented with 10% whole blueberry (BB) powder for two weeks beginning on postnatal day 21 (PND21) significantly increased bone formation at PND35. However, the minimal level of dietary BB needed to produce these effects is, as yet, unknown. The current study examined three different levels diet supplementation (1, 3, and 5%) 35 days PND25 quality, osteoclastic resorption in female rats. Peripheral...