A5101418532- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Space Technology and Applications
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Chemokine receptors and signaling
- DNA Repair Mechanisms
- Cell Adhesion Molecules Research
- melanin and skin pigmentation
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Pulsed Power Technology Applications
- Multiple Myeloma Research and Treatments
- Particle Detector Development and Performance
- Mitochondrial Function and Pathology
- Phagocytosis and Immune Regulation
- Calcium signaling and nucleotide metabolism
- Semiconductor materials and devices
- Proteoglycans and glycosaminoglycans research
- Polyamine Metabolism and Applications
- Pluripotent Stem Cells Research
Beijing Institute of Genomics
2017-2024
Chinese Academy of Sciences
2017-2024
University of Chinese Academy of Sciences
2017-2024
Shanghai Center For Bioinformation Technology
2024
The Third Affiliated Hospital of Zhejiang Chinese Medical University
2024
Qingdao Agricultural University
2024
Qingdao Academy of Agricultural Sciences
2024
Shenzhen Second People's Hospital
2024
Shenzhen University
2024
Yue Bei People's Hospital
2024
PRKN/parkin activation through phosphorylation of its ubiquitin and ubiquitin-like domain by PINK1 is critical in mitophagy induction for eliminating the damaged mitochondria. Deubiquitinating enzymes (DUBs) functionally reversing PRKN ubiquitination are controlling magnitude PRKN-mediated process. However, potential DUBs that directly target antagonize pro-mitophagy effect remains to be identified characterized. Here, we demonstrated USP33/VDU1 localized at outer membrane mitochondria...
We present a new ETSOI CMOS integration scheme. The process flow incorporates all benefits from our previous unipolar work. Only single mask level is required to form raised source/drain (RSD) and extensions for both NFET PFET. Another feature of this work the incorporation two strain techniques boost performance, (1) Si:C RSD SiGe PFET, (2) enhanced stress liner effect coupling with faceted RSD. Using boosters we demonstrate PFET drive currents 640 490 ¿A/¿m, respectively, at I <sub...
Abstract There is often interest in dissecting the relative contributions of N-glycans, O-glycans and glycosphingolipids (GSLs) regulating complex biological traits like cell signaling, adhesion, development metastasis. To address this, we developed a CRISPR-Cas9 toolkit to selectively truncate each these commonly expressed glycan-types. Here, O-glycan biosynthesis was truncated by knocking-out Core 1 β3Gal-T Specific Molecular Chaperone ( COSMC ), N-glycans targeting β1,2 GlcNAc-transferase...
Multiple myeloma (MM) growth is supported by an immune-tolerant bone marrow microenvironment. Here, we find that loss of Never in mitosis gene A (NIMA)-related kinase 2 (NEK2) tumor microenvironmental cells associated with MM suppression. The absence NEK2 leads to both fewer tumor-associated macrophages (TAMs) and inhibitory T cells. expression myeloid progenitor promotes the generation functional TAMs when stimulated conditional medium. Clinically, high increased CD8+ effector memory cells,...
Human RecQL4 helicase plays critical roles in the maintenance of genomic stability. Mutations results three clinically related autosomal recessive disorders: Rothmund-Thomson syndrome (RTS), RAPADILINO, and Baller-Gerold syndrome. In addition to several premature aging features, RTS patients are characterized by aneuploidy involving either loss or gain a single chromosome. Chromosome mosaicism isochromosomes chromosomes 2, 7, 8 have been reported RecQL4-deficient patients, but precise role...
Abstract Glycan biosynthesis on cell surface proteins and lipids is orchestrated by different classes of enzymes including the following: i. glycosyltransferases that add saccharides; ii. glycosidases trim glycans; iii. conserved oligomeric golgi complex members regulate intracellular transport; iv. aiding sugar–nucleotides; v. sulfotransferases. This manuscript describes a pooled “glycoGene CRISPR” lentiviral library targets 347 human genes involved in above processes. Approximately 10...
We recently discovered that human neutrophils express immunomodulatory glycoproteins carrying unusual and highly truncated paucimannosidic N-glycans (Man1-3GlcNAc2Fuc0-1), but their biosynthesis remains elusive. Guided by the well-characterized truncation pathway in invertebrates plants which N-acetyl-β-D-hexosaminidase (Hex) isoenzymes catalyze protein (PMP) formation, we here set out to test if homologous Hex α β subunits encoded HEXA HEXB drive a similar neutrophils. To this end,...
Creatine is a naturally occurring derivative of an amino acid commonly utilized in functional foods and pharmaceuticals. Nevertheless, the current industrial synthesis creatine relies on chemical processes, which may hinder its utilization certain applications. Therefore, biological approach was devised that employs whole-cell biocatalysis bacterium Corynebacterium glutamicum, considered safe for use food production, to produce safe-for-consumption creatine. The objective this study identify...
The immune response relies on the integration of cell-intrinsic processes with cell-extrinsic cues. During infection, B cells vacate marrow during emergency granulopoiesis but return upon restoration homeostasis. Here we report a novel glycosylation-mediated crosstalk between and hematopoietic progenitors. Human secrete active ST6GAL1 sialyltransferase that remodels progenitor cell surface glycans to suppress granulopoiesis. In mouse models, from alters sialylation profile bone populations,...
Exome and deep sequencing of cells treated with a panel lentiviral guide RNA demonstrate that both on- off-target editing proceed in time-dependent manner. Thus, methods to temporally control Cas9 activity would be beneficial. To address this need, we describe "self-inactivating CRISPR (SiC)" system consisting single deactivates the Streptococcus pyogenes nuclease doxycycline-dependent This enables defined, temporal any cell type also vivo. Results show SiC may enable reduction editing, less...
Transmembrane protein 52B (TMEM52B), a newly identified tumor-related gene, has been reported to regulate various tumors, yet its role in nasopharyngeal carcinoma (NPC) remains unclear. Transcriptomic analysis of NPC cell lines reveals frequent overexpression TMEM52B, and immunohistochemical results show that TMEM52B is associated with advanced tumor stage, recurrence, decreased survival time. Depleting inhibits the proliferation, migration, invasion, oncogenesis cells vivo. encodes two...
Abstract Ubiquitination-dependent DNA damage response (DDR) signals play a critical role in the cellular choice of repair pathways. Human helicase RecQL4 participates replication and repair, loss is associated with autosomal recessive genetic disorders characterized by genomic instability features. In an earlier study, was isolated as stable complex that contained two ubiquitin ligases N-end rule (UBR1 UBR2). However, it unknown whether or not ubiquitination status for its function. Here, we...
MMS19 localizes to the cytoplasmic and nuclear compartments involved in transcription nucleotide excision repair (NER). However, whether mitochondria, where it plays a role maintaining mitochondrial genome stability, remains unknown. In this study, we provide first evidence that is localized inner membrane of mitochondria participates mtDNA oxidative damage repair. knockdown led dysfunctions including decreased copy number, diminished capacity, elevated levels common deletion after stress....
Abstract Here a compact three orthogonal planes high-energy electron radiography system was proposed. One of the critical technologies, ultra-fast beam bunches split from bunch train are studied. The separated could be transported to target for dynamic diagnostics. key elements transverse deflecting cavity (TDC) and twin septum magnet (TSM). principle TDC TSM briefly introduced. An example test experiment (40 MeV beam) with is designed studied by particle-tracking simulation it confirms this...
The shortage of human hepatocytes continues to be a significant limitation for the widespread application hepatocyte transplantation and bioartificial liver (BAL) support therapy. Recombinant activation gene 2 (Rag2) fumarylacetoacetate hydrolase (Fah)-deficient mice could highly repopulated with hepatocytes. However, Fah/Rag2-deficient can only produce up 1 × 108 per mouse. We hypothesized that 2-10 1010 produced pig, which is an adequate supply BAL In novel approach, we used stably...
Abstract High-energy electron radiography (HEER) has been proposed for time-resolved imaging of materials, high-energy density matter, and inertial confinement fusion. The areal-density resolution, determined by the image intensity information is critical these types diagnostics. Preliminary experimental studies different materials with same thickness target have imaged analyzed. Although there are some discrepancies between theory analysis, results show that distribution can indeed be...
A distributed transversal filter (DTF) is a good candidate for ultrafast pulse shaping in wideband systems like UWB. This paper presents the circuit analysis of DTFs based on transmission line theory. We also show detailed design procedure DTFs. 5-tap prototype DTF designed and fabricated using microwave PCB substrate pHEMT discrete transistors. Simulation initial measurement results demonstrate its capability.
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Abstract Background Transient Receptor Potential Mucolipin 1 (TRPML1) serves as a pivotal reactive oxygen species (ROS) sensor in cells, which is implicated the regulation of autophagy. However, its function melanocyte autophagy under oxidative stress remains elusive. Methods The expression and ion channel TRPML1 were investigated using immunofluorescence calcium imaging primary human melanocytes (MCs). After activating with MLSA1 (TRPML1 agonist), autophagy‐related molecules via western...
ABSTRACT The de‐ubiquitinase USP33 has been shown to possess either tumour‐promoting or inhibitory effect on human cancer cells. However, all these findings are mainly based in vitro cell culture models, and the vivo evidence, which is more plausible digest functional role of carcinogenic process, still lacking. Here, we demonstrate that modulates DNA damage responses including cycle arrest apoptosis induction through associating with p53. It directly interacts p53 mediate its...